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Types A and D Trichothecene Mycotoxins from the Fungus Myrothecium roridum
Author(s)
Lakornwong, Waranya
Kanokmedhakul, Kwanjai
Unartngam, Arm
Tontapha, Sarawut
Amornkitbamrung, Vittaya
Kanokmedhakul, Somdej
Date Issued
July 1, 2019
Type
Article
Abstract
Chromatographic separation of extracts from the fungal biomass of a plant pathogenic fungus, Myrothecium roridum, yielded 8 trichothecene toxins including 6 type D trichothecenes (1 - 6) and 2 type A trichothecenes (7 - 8). 6′,12′-Epoxymyrotoxin A (1) and 7′-hydroxymytoxin B (2) were new macrocyclic trichothecenes, while the other trichothecenes were identified as myrotoxin B (3), myrotoxin D hydrate (4), 2′,3′-epoxymyrothecine A (5), miotoxin A (6), and 2 trichothecenes lacking the macrocyclic lactone system, roridin L-2 (7) and trichoverritone (8). The structures of these mycotoxins were characterized using spectroscopic methods. The absolute configurations of 1 and 2 were determined by NOESY and a comparison of their experimental and calculated ECD spectra. Most of these mycotoxins (1 - 4 and 6) exhibited highly potent antimalarial activity against Plasmodium falciparum. They also showed strong cytotoxicity towards KB and NCI-H187 cell lines (IC 50 0.60-112.28 nM), as well as the Vero cell line (IC 50 1.50-46.51 nM).
Citation
Planta Medica, 85(9-10), 774-780, 2019