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Paenibacillus lemnae sp. nov., an endophytic bacterium of duckweed (Lemna aequinoctialis)
Date Issued
January 1, 2015
Type
Article
Abstract
A Gram-stain-variable, rod-shaped and endospore-forming bacterium, designated strain L7-75, was isolated from duckweed (Lemna aequinoctialis). Cells were motile with a monopolar flagellum. Phylogenetic analysis of the 16S rRNA gene sequence indicated that strain L7-75T belonged to the genusPaenibacillus, and the closest phylogenetically related species werePaenibacillus uliginis N3/975T (98.5 % 16S rRNA gene sequence similarity),Paenibacillus purispatii ES_M17T (98.5 %), Paenibacillus lactis MB 1871T (98.2 %), Paenibacillus campinasensis 324T (97.7 %), Paenibacillus glucanolyticusS93T (97.7 %) and Paenibacillus lautus ATCC 43898T (97.4 %). Growth of strain L7-75T was observed at pH 7–10 and at 20–40 °C, and NaCl concentrations up to 5 % (w/v) were tolerated. Major cellular fatty acids included anteiso-C15 : 0, C16 : 0 and anteiso-C17 : 0 that were present at 36.0 %, 14.2 % and 10.0 % of the total cellular fatty acid profile, respectively. The major polar lipids were diphosphatidylglycerol, phosphatidylglycerol, phosphatidylethanolamine and phosphatidyl-N-methylethanolamine. MK-7 was the predominant menaquinone. The diamino acid found in the cell-wall peptidoglycan wasmeso-diaminopimelic acid. The DNA G+C content was 49.1 mol% (Tm). DNA–DNA relatedness values between strain L7-75T and its closest relatives ranged from 4.4 to 47.8 %. These results indicate that strain L7-75T represents a novel species of the genus Paenibacillus, for which the name Paenibacillus lemnaesp. nov. is proposed. The type strain is L7-75T ( = BCC 67838T = NBRC 109972T).
Citation
International Journal of Systematic and Evolutionary Microbiology, 65(1), 107-112, 2015