Enhanced high β-carotene yeast cell production by Rhodotorula paludigena CM33 and in vitro digestibility in aquatic animals

Thumkasem, Namphet; On-mee, Thapanut; Kongsinkaew, Chatchol; Chittapun, Supenya; Pornpukdeewattana, Soisuda; Ketudat-Cairns, Mariena; Thongprajukaew, Karun; Antimanon, Sompot; Charoenrat, Theppanya

doi:10.1038/s41598-024-59809-7

Enhanced high β-carotene yeast cell production by Rhodotorula paludigena CM33 and in vitro digestibility in aquatic animals

Author(s)

Thumkasem, Namphet

On-mee, Thapanut

Kongsinkaew, Chatchol

Chittapun, Supenya

Pornpukdeewattana, Soisuda

Ketudat-Cairns, Mariena

Thongprajukaew, Karun

Antimanon, Sompot

Charoenrat, Theppanya

Date Issued

December 1, 2024

Type

Article

DOI

10.1038/s41598-024-59809-7

Abstract

This study assessed Rhodotorula paludigena CM33's growth and β-carotene production in a 22-L bioreactor for potential use as an aquatic animal feed supplement. Optimizing the feed medium's micronutrient concentration for high-cell-density fed-batch cultivation using glucose as the carbon source yielded biomass of 89.84 g/L and β-carotene concentration of 251.64 mg/L. Notably, using sucrose as the carbon source in feed medium outperforms glucose feeds, resulting in a β-carotene concentration of 285.00 mg/L with a similar biomass of 87.78 g/L. In the fed-batch fermentation using Sucrose Feed Medium, R. paludigena CM33 exhibited high biomass production rates (Qx) of 0.91 g/L.h and remarkable β-carotene production rates (Qp) of 2.97 mg/L.h. In vitro digestibility assays showed that R. paludigena CM33, especially when cultivated using sucrose, enhances protein digestibility affirming its suitability as an aquatic feed supplement. Furthermore, R. paludigena CM33's nutrient-rich profile and probiotic potential make it an attractive option for aquatic nutrition. This research highlights the importance of cost-effective carbon sources in large-scale β-carotene production for aquatic animal nutrition.

Citation

Scientific Reports, 14(1), 2024