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A new coumarin-based “OFF–ON” fluorescent sensor for H2S detection in HeLa cells
Author(s)
Date Issued
February 5, 2025
Type
Article
Abstract
A new “OFF–ON” coumarin-based fluorescent probe for H2S detection was designed and successfully developed through O-sulfonylation between a dabsyl quencher and 7-hydroxy-4-methylcoumarin as a fluorescent reporter, based on a FRET approach. This H2S responsive probe, utilizing H2S assisted thiolysis of a sulfonate ester as the sensing strategy, demonstrated excellent performance towards H2S with a limit of detection (LoD) of 1.64 µM, along with superb selectivity, good stability and high specificity towards H2S without interference from other biomarkers and analytes. Moreover, dabsyl-7-hydroxy-4-methylcoumarin (dabsylcoumarin) is capable of permeating the cell membrane and effectively visualizing the level of H2S in the living HeLa cells without cytotoxicity.
Citation
Spectrochimica Acta Part A Molecular and Biomolecular Spectroscopy, 326, 2025