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Urinary dengue NS1 detection on Au-decorated ZnO nanowire platform
Author(s)
Sitthisuwannakul, Kannika
Sukthai, Ratchanon
Zhu, Zetao
Nagashima, Kazuki
Chattrairat, Kunanon
Phanthanawiboon, Supranee
Klamchuen, Annop
Baba, Yoshinobu
Yasui, Takao
Date Issued
June 15, 2024
Type
Article
Abstract
Biodetection for non-invasive diagnostics of fluids, especially urine, remains a challenge to scientists due to low target concentrations. And biological complexes of the detection target may contain contaminants that also interfere with any assay. Dengue non-structural 1 protein (Dengue NS1) is an important biomarker for dengue hemorrhagic fever and dengue shock syndrome. Here, we developed an Au-decorated nanowire platform and applied it with a sandwich fluorophore-linked immunosorbent well plate assay (FLISA) to detect Dengue NS1 in urine. For the platform, we fabricated zinc oxide (ZnO) nanowires to provide a high surface area and then coated them with gold nanoparticles (ZnO/Au nanowires) to simply modify the Dengue NS1 antibody and enhance the fluorescence intensity. Our platform employs a sandwich FLISA that exhibits high sensitivity, specifically detecting Dengue NS1 with a limit of detection (LOD) of 1.35 pg/mL. This LOD was 4500-fold lower than the LOD of a commercially available kit for Dengue NS1 enzyme-linked immunosorbent assay. We believe that our ZnO/Au nanowire platform has the potential to revolutionize the field of non-invasive diagnostics for dengue.
Citation
Biosensors and Bioelectronics, 254, 2024