Rahong, Sakon

Preferred name

Rahong, Sakon

Alternative Name

Rahong, S.

Main Affiliation

Integrated Innovative Technology

sakon.ra@kmitl.ac.th

Now showing 1 - 10 of 38

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Metal Oxide Nanostructures Enhanced Microfluidic Platform for Efficient and Sensitive Immunofluorescence Detection of Dengue Virus
(2023-11-01)
Pormrungruang, Pareesa
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Phanthanawiboon, Supranee
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Jessadaluk, Sukittaya
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Larpthavee, Preeda
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Thaosing, Jiraphon
Rapid and sensitive detection of Dengue virus remains a critical challenge in global public health. This study presents the development and evaluation of a Zinc Oxide nanorod (ZnO NR)-surface-integrated microfluidic platform for the early detection of Dengue virus. Utilizing a seed-assisted hydrothermal synthesis method, high-purity ZnO NRs were synthesized, characterized by their hexagonal wurtzite structure and a high surface-to-volume ratio, offering abundant binding sites for bioconjugation. Further, a comparative analysis demonstrated that the ZnO NR substrate outperformed traditional bare glass substrates in functionalization efficiency with 4G2 monoclonal antibody (mAb). Subsequent optimization of the functionalization process identified 4% (3-Glycidyloxypropyl)trimethoxysilane (GPTMS) as the most effective surface modifier. The integration of this substrate within a herringbone-structured microfluidic platform resulted in a robust device for immunofluorescence detection of DENV-3. The limit of detection (LOD) for DENV-3 was observed to be as low as 3.1 × 10−4 ng/mL, highlighting the remarkable sensitivity of the ZnO NR-integrated microfluidic device. This study emphasizes the potential of ZnO NRs and the developed microfluidic platform for the early detection of DENV-3, with possible expansion to other biological targets, hence paving the way for enhanced public health responses and improved disease management strategies.
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Annealed zno/al2o3 core-shell nanowire as a platform to capture rna in blood plasma
(2021-07-01)
Takahashi, Hiromi
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Yasui, Takao
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Klamchuen, Annop
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Khemasiri, Narathon
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Wuthikhun, Tuksadon
RNA analytical platforms gained extensive attention recently for RNA-based molecular analysis. However, the major challenge for analyzing RNAs is their low concentration in blood plasma samples, hindering the use of RNAs for diagnostics. Platforms that can enrich RNAs are essential to enhance molecular detection. Here, we developed the annealed ZnO/Al<inf>2</inf>O<inf>3</inf> core-shell nanowire device as a platform to capture RNAs. We showed that the annealed ZnO/Al<inf>2</inf>O<inf>3</inf> core-shell nanowire could capture RNAs with high efficiency compared to that of other circulating nucleic acids, including genomic DNA (gDNA) and cell-free DNA (cfDNA). Moreover, the nanowire was considered to be biocompatible with blood plasma samples due to the crystalline structure of the Al<inf>2</inf>O<inf>3</inf> shell which serves as a protective layer to prevent nanowire degradation. Our developed device has the potential to be a platform for RNA-based extraction and detection.
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Modulation spectroscopy study of inorganic-organic hybrid quantum well-like nanostructures
(2007-08-28)
Nukeaw, J.
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Rahong, S.
The optical transition energy in zinc selenide (ZnSe)/tris(8- hydroxyquinoline) aluminum (Alq3) /N,N'-Bis(3-methyphenyl)-N,N -diphenylbenzidine inorganic-organic hybrid quantum well-like nanostructures (hybrid QW-like nanostructure) with well thickness of Aq3 from 10 to 50 nm were investigated by modulation spectroscopy as electroreflectance (ER) measurements. ER features due to optical transition energy were observed in the hybrid QW of well thickness. The transition energies were determined by fitting the ER spectra to the theoretical line-shape expression. The subband transition energy decreased with increasing well thickness might be due to the increase of well results in the reduction of quantum confinement energy. © 2007 IEEE.
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Tailoring ZnO Nanostructures through Precursor Concentration and Hydrothermal Duration: A Pathway to Efficient Solar Water Splitting
(2026-11-10)
Borklom, Phanlapa
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Khemasiri, Narathon
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Jessadaluk, Sukittaya
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Rattanawarinchai, Prapakorn
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Kayunkid, Navaphun
This work investigated the formation of ZnO nanostructures on ITO substrates prepared by self-seeding hydrothermal synthesis for photoelectrochemical ( PEC) water splitting applications. The hydrothermal parameters, precursor concentration and hydrothermal time, were varied to explore their influences on ZnO crystallinity, morphology, and PEC performance. The combinations of X-ray diffraction and field emission scanning electron microscopy revealed highly oriented ZnO nanostructures with diverse morphologies, including small granules, nanorods, dense films, and hexagonal platelets. Topographic profiling of the morphological parameters revealed complex relationships between synthesis conditions and nanostructure characteristics, highlighting the importance of considering aggregation phenomena in substrate-based growth. This aggregation led to deviations from conventional crystal growth theory predictions, particularly for grain density and diameter evolution. PEC performance evaluation identified ZnO nanorods as the optimal morphology, exhibiting a photocurrent density of 0.182 mA/cm² at 0 V vs. Ag/AgCl. Further enhancement was achieved by decorating ZnO nanorods with CdS nanoparticles, resulting in a six-fold increase in photocurrent density (1.2 mA/cm²). This improvement is attributed to expanded light absorption and improved charge separation at the CdS/ ZnO interface. Our findings demonstrate the potential of rationally designed ZnO-based nanostructures in the advancement of solar-driven water splitting technologies and provide valuable insights for optimizing PEC systems through precise control of hydrothermal synthesis parameters, consideration of substrate-induced aggregation, and strategies for photoelectrochemical (PEC) water splitting applications.
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Growth window and metal-insulator transition behavior of VO2 thin films deposited by pulsed laser deposition for thermal switch applications
(2026-05-01)
Jessadaluk, Sukittaya
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Rahong, Sakon
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Kayunkid, Navaphun
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Khemasiri, Narathon
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Rangkasikorn, Adirek
Vanadium dioxide (VO<inf>2</inf>) is a strongly correlated transition metal oxide that exhibits a sharp and reversible metal-insulator transition (MIT) near room temperature, making it a promising material for thermal switching and adaptive electronic applications. In this study, VO<inf>2</inf> thin films were deposited on single-crystalline Si, thermally grown SiO<inf>2</inf>, and fused quartz substrates by pulsed laser deposition, and the influence of substrate temperature and oxygen partial pressure on phase formation, structural properties, and MIT behavior was systematically investigated. By optimizing deposition conditions within a narrow oxygen pressure window, phase-pure monoclinic VO<inf>2</inf>(M) thin films with high crystalline quality were achieved while suppressing the formation of over-oxidized vanadium oxide phases. Structural and chemical analyses using X-ray diffraction, Raman spectroscopy, and X-ray photoelectron spectroscopy confirmed the stabilization of the V4+ oxidation state and uniform film stoichiometry. Temperature-dependent electrical measurements revealed a pronounced and reproducible MIT characterized by an abrupt change in resistance and a clear thermal hysteresis. In-situ temperature-dependent X-ray diffraction further demonstrated a direct correlation between the monoclinic-rutile structural transformation and the electronic transition. Importantly, the MIT behavior was consistently observed across all investigated substrates, indicating robust film growth and substrate tolerance. These results provide insight into the structure-property relationships governing VO<inf>2</inf> thin films and highlight their potential for integration into thermal switch and thermally adaptive device architectures.
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Modification of the optical properties of polydimethylsiloxane (PDMS) for photonic crystal biosensor application
(2010-05-05)
Rahong, Sakon
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Saekow, Bunpot
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Porntheerapat, Suppanit
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Nukeaw, Jiti
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Hruanun, Chandech
The sensitivity of a photonic crystal optical biosensor device can be enhanced through the difference in effective refractive index of dielectric materials. In this work, we report the optical modification of refractive index of PDMS from 1.41 to above, by incorporating the additive materials to enhance the sensitivity of photonic crystal optical biosensor devices. The refractive index of mixed-PDMS materials are measured by ellipsometry technique. The surface and cross section morphologies are characterized by Field-Emission Scanning Electron Microscope (FESEM) and Atomic Force Microscope (AFM). The grating structure of 800 nm period PDMS with low refractive index was fabricated by Nano-Imprinting Lithography (NIL) then the mixed-PDMS with high refractive index was spun on the periodic PDMS as photonic crystal device. The sensitivity of devices is characterized and demonstrated by reflection spectra intensity of fluids on surface of the devices. ©2010 IEEE.
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Engineering Nanowire-Mediated Cell Lysis for Microbial Cell Identification
(2019-02-26)
Yasui, Takao
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Yanagida, Takeshi
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Shimada, Taisuke
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Otsuka, Kohei
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Takeuchi, Masaki
Researchers have demonstrated great promise for inorganic nanowire use in analyzing cells or intracellular components. Although a stealth effect of nanowires toward cell surfaces allows preservation of the living intact cells when analyzing cells, as a completely opposite approach, the applicability to analyze intracellular components through disrupting cells is also central to understanding cellular information. However, the reported lysis strategy is insufficient for microbial cell lysis due to the cell robustness and wrong approach taken so far (i.e., nanowire penetration into a cell membrane). Here we propose a nanowire-mediated lysis method for microbial cells by introducing the rupture approach initiated by cell membrane stretching; in other words, the nanowires do not penetrate the membrane, but rather they break the membrane between the nanowires. Entangling cells with the bacteria-compatible and flexible nanowires and membrane stretching of the entangled cells, induced by the shear force, play important roles for the nanowire-mediated lysis to Gram-positive and Gram-negative bacteria and yeast cells. Additionally, the nanowire-mediated lysis is readily compatible with the loop-mediated isothermal amplification (LAMP) method because the lysis is triggered by simply introducing the microbial cells. We show that an integration of the nanowire-mediated lysis with LAMP provides a means for a simple, rapid, one-step identification assay (just introducing a premixed solution into a device), resulting in visual chromatic identification of microbial cells. This approach allows researchers to develop a microfluidic analytical platform not only for microbial cell identification including drug- and heat-resistance cells but also for on-site detection without any contamination.
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Phase evolution in annealed Ni-doped WO3 nanorod films prepared via a glancing angle deposition technique for enhanced photoelectrochemical performance
(2022-05-15)
Wattanawikkam, Chakkaphan
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Bootchanont, Atipong
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Porjai, Porramain
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Jetjamnong, Chanthawut
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Kowong, Rattanachai
Ni-doped WO<inf>3</inf> nanorod films were fabricated via a reactive magnetron cosputtering with a glancing angle deposition technique. The crystal structure and surface morphology were observed using grazing incident X-ray diffraction and field emission Scanning Electron Microscopy, respectively. The chemical compositions and oxidation state of each element were investigated by X-ray photoemission spectroscopy. The local structure and phase evolution were investigated via X-ray absorption spectroscopy. The local structure of Ni atoms in WO<inf>3</inf> nanorod films is characterized as a NiWO<inf>4</inf> nanocluster in the WO<inf>3</inf> matrix, which is supported by calculated spectra. The phase information obtained after annealing demonstrates that short-length order in amorphous transitions to crystallinity. The phase information of the local structures were acquired and discussed as well as the effect on the annealing process. The coupling of amorphous WO<inf>3</inf>, crystalline WO<inf>3</inf>, and amorphous NiWO<inf>4</inf> exhibits high photoelectrochemical activity of the sample annealed at 400 °C, which was observed with a large current density (2.35 μA/cm2) at 1.20 V vs. Ag/AgCl under visible light irradiation, which is greater than that of the unmodified WO<inf>3</inf> photoelectrode (1.38 μA/cm2).
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Systematic investigations on morphological properties of aluminum-doped zinc oxide transparent electrode prepared from pulsed laser deposition and its electrochromic application
(2023-03-01)
Khemasiri, Narathon
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Klamchuen, Annop
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Jessadaluk, Sukittaya
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Rattanawarinchai, Prapakorn
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Borklom, Punlapa
Transparent electrode (TE) is considered as one of the fundamental components in the optoelectronics. The surface of TE layer plays a crucial role in the charge-transport characteristics. Herein, a systematic investigation on the morphological properties of the laser-ablated aluminum-doped zinc oxide (AZO) films as a TE material prepared from various laser fluences is demonstrated. It is revealed that the electrical properties of AZO films are strongly associated with their surface properties rather than that of the bulk film. As the laser fluence increased, the concentration of Al-dopant in AZO films is decreased which directly impacts on the electrical properties. Such vanishment is originated from the bombardment of the incident particles/ions with excessive kinetic energy on film's surface during the deposition. Moreover, the optimized AZO film with low resistivity (1.13 × 10−3 Ω cm) and high optical transmittance (over 90%) achieved from controlling the laser fluence at 0.7 J/cm2 is employed as TE layer in the electrochromic device. Our results highlight that the surface properties of TE layer are very critical for electronic performance.
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Microheater-integrated zinc oxide nanowire microfluidic device for hybridization-based detection of target single-stranded DNA
(2021-06-18)
Takahashi, Hiromi
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Yasui, Takao
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Kashida, Hiromu
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Makino, Koki
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Shinjo, Keiko
Detection of cell-free DNA (cfDNA) has an impact on DNA analysis in liquid biopsies. However, current strategies to detect cfDNA have limitations that should be overcome, such as having low sensitivity and requiring much time and a specialized instrument. Thus, non-invasive and rapid detection tools are needed for disease prevention and early-stage treatment. Here we developed a device having a microheater integrated with zinc oxide nanowires (microheater-ZnO-NWs) to detect target single-stranded DNAs (ssDNAs) based on DNA probe hybridization. We confirmed experimentally that our device realized in-situ annealed DNA probes by which we subsequently detected target ssDNAs. We envision that this device can be utilized for fundamental studies related to nanobiodevice-based DNA detection.

Rahong, Sakon

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