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An Automated Colony Counter for Serial-Dilution Culture Method

Author(s)
Vongmanee, Naphatsawan
Bunmak, Aonjira
Visitsattapongse, Sarinporn
Date Issued
May 16, 2018
Type
Conference Paper
DOI
10.1145/3232059.3232074
Abstract
Serial-dilution method is used for estimating the concentration of unknown sample by counting the number of bacterial colonies cultured and then backtracking the measured counts to the unknown concentration. This process is time-consuming, error prone and cause eye fatigue. This paper proposes an automatic colony counter with captured image using webcam camera and uses Raspberry Pi for processing with capturing, thresholding, circular Hough transform, watershed segmentation and displaying results number of colonies on culture media.
Citation
ACM International Conference Proceeding Series, 42-45, 2018
Subjects

Bacterial colony

Circular hough transf...

Serial-dilution

Watershed segmentatio...

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