Now showing 1 - 10 of 43
  • Some of the metrics are blocked by your 
    Item type:Publication,
    Store-operated calcium entry facilitates LPS-induced superoxide anion-dependent macrophage extracellular traps
    (2025-07-09)
    Nguyen, Thang Ngoc
    ;
    Lin, Tzu Chien
    ;
    Chimphlee, Waratchaya
    ;
    Siew, Kon Xuen
    ;
    Vongmanee, Naphatsawan
    Macrophage extracellular traps (METs) represent a recently discovered complex defence mechanism that is distinct from phagocytosis and involves the release of DNA and antibacterial proteins. They play an important role in pathogen removal, and calcium ions (Ca 2+) have also been reported to be involved. In the present study, we identified METotic cells using digitonin as an alternative to Triton X-100, coupled with immunofluorescence staining using lamin antibodies. The limited permeability of digitonin ensures exclusive intranuclear antibody labelling of MET cells, therefore providing a straightforward and intuitive differentiation method. We found that under lipopolysaccharide stimulation, macrophages undergo store-operated Ca 2+ entry (SOCE) to facilitate Ca 2+ influx. Elevation of cytoplasmic Ca 2+ levels by SOCE promotes the generation of superoxide anions by NADPH oxidase (NOX), ultimately leading to METosis. In summary, our study strengthens the role of Ca 2+ in NOX-dependent METosis, which differs from previous studies focusing on Ca 2+ in the NOX-independent pathway. Our research reveals that Ca 2+ -mediated regulation of NOX plays a crucial role in METosis, especially in SOCE, and provides novel ideas for future research.
  • Some of the metrics are blocked by your 
    Item type:Publication,
    Analysis of Deep Learning-Based Phase Retrieval Algorithm Performance for Quantitative Phase Imaging Microscopy
    (2022-05-01) ;
    Thadson, Kitsada
    ;
    Pechprasarn, Suejit
    ;
    Thongpance, Nuntachai
    Quantitative phase imaging has been of interest to the science and engineering community and has been applied in multiple research fields and applications. Recently, the data-driven approach of artificial intelligence has been utilized in several optical applications, including phase retrieval. However, phase images recovered from artificial intelligence are questionable in their correctness and reliability. Here, we propose a theoretical framework to analyze and quantify the performance of a deep learning-based phase retrieval algorithm for quantitative phase imaging microscopy by comparing recovered phase images to their theoretical phase profile in terms of their correctness. This study has employed both lossless and lossy samples, including uniform plasmonic gold sensors and dielectric layer samples; the plasmonic samples are lossy, whereas the dielectric layers are lossless. The uniform samples enable us to quantify the theoretical phase since they are established and well understood. In addition, a context aggregation network has been employed to demonstrate the phase image regression. Several imaging planes have been simulated serving as input and the label for network training, including a back focal plane image, an image at the image plane, and images when the microscope sample is axially defocused. The back focal plane image plays an essential role in phase retrieval for the plasmonic samples, whereas the dielectric layer requires both image plane and back focal plane information to retrieve the phase profile correctly. Here, we demonstrate that phase images recovered using deep learning can be robust and reliable depending on the sample and the input to the deep learning.
  • Some of the metrics are blocked by your 
    Item type:Publication,
    Biosensors for Bacillus sphaericus detection to diagnostic Pseudotumor of the lung
    (2023-01-01)
    Vongmanee, Naphatsawan
    ;
    In the present for diagnostic Pseudotumor in the lungs, X-ray image can help for diagnostic to the patient but could not approval the patient has got infection with bacteria type of Bacillus sphaericus. Diagnosis way of Pseudotumor in the lung by cause for this type of bacteria is very difficult because lesions are shown as pulmonary nodules or masses in which the border can be characteristically well distinguished on X-ray cannot approve infection with Bacillus sphaericus. For diagnostic need to confirm with result from laboratory and have a long time for get the result. So in this paper we will study about biosensors with molecular imprinting polymer for Bacillus sphaericus to examine the diagnosis and treatment of this disease for patient
  • Some of the metrics are blocked by your 
    Item type:Publication,
    Automated segmentation of infarct lesions in t1‐weighted mri scans using variational mode decomposition and deep learning
    Automated segmentation methods are critical for early detection, prompt actions, and immediate treatments in reducing disability and death risks of brain infarction. This paper aims to develop a fully automated method to segment the infarct lesions from T1‐weighted brain scans. As a key novelty, the proposed method combines variational mode decomposition and deep learning-based segmentation to take advantages of both methods and provide better results. There are three main technical contributions in this paper. First, variational mode decomposition is applied as a pre-processing to discriminate the infarct lesions from unwanted non‐infarct tissues. Second, overlapped patches strategy is proposed to reduce the workload of the deep‐learning‐based segmentation task. Finally, a three‐dimensional U‐Net model is developed to perform patch‐wise segmentation of infarct lesions. A total of 239 brain scans from a public dataset are utilized to develop and evaluate the proposed method. Empirical results reveal that the proposed automated segmentation can provide promising performances with an average dice similarity coefficient (DSC) of 0.6684, intersection over union (IoU) of 0.5022, and average symmetric surface distance (ASSD) of 0.3932, respectively.
  • Some of the metrics are blocked by your 
    Item type:Publication,
    Automated Bacterial Colony Counting on Agar Plate
    (2023-01-01)
    Bunkum, Manao
    ;
    In several fields, such as microbiology research, medical diagnostics, and food safety evaluation, bacterial colony counting is extremely important. However, the method of manual counting is time-consuming, labor-intensive, and prone to human error. This research approached these problems by using MATLAB's image processing feature to automatically count the number of bacterial colonies on agar plates. This technique effectively detects bacterial colonies from photos of agar plates by using image analysis algorithms. The images of agar plates were captured while controlling the lighting and adjusting the size to achieve the highest possible image quality. This study encompassed 10 bacterial species, achieving an accuracy of approximately 80%. This level of precision underscores the reliability and effectiveness of our automated system.
  • Some of the metrics are blocked by your 
    Item type:Publication,
    Optical-based foot plantar pressure measurement with application in human postural balance, gait and recognition analysis
    In this research, we purposed the design of real-time low-cost marker-free optical-based plantar-pressure measurement with application in human postural control, gait and recognition analysis. The system consists of a series of digital cameras capture installed underneath the acrylic-top platform. Light of LED strip mounted along the side of the acrylic plate deflected by the pressure exerted between the glossy white paper installed in the top of acrylic and the acrylic plate to the digital cameras provides the color-coded plantar pressure image of the subject standing on the platform. The system can provide both static features and dynamic features. Our hybrid system consists of a series of USB cameras aligned under the acrylic-plate walking platform. The mosaic image processing is used to concatenate the captured image to increase the sensing area. Captured the image data in video mode, various dynamic parameters can be derived for further dynamic analysis. The image capturing in one specific frame can be used for static analysis. Application in human-postural measurement, gait analysis, and person identification indicate that our system is an all-in-one system for plantar pressure measurement.
  • Some of the metrics are blocked by your 
    Item type:Publication,
    Cry4Aa and Cry4Ba Mosquito-Active Toxins Utilize Different Domains in Binding to a Particular Culex ALP Isoform: A Functional Toxin Receptor Implicating Differential Actions on Target Larvae
    (2022-10-01)
    Dechkla, Manussawee
    ;
    Charoenjotivadhanakul, Sathapat
    ;
    Imtong, Chompounoot
    ;
    ;
    Li, Hui Chun
    The three-domain Cry4Aa toxin produced from Bacillus thuringiensis subsp. israelensis was previously shown to be much more toxic to Culex mosquito larvae than its closely related toxin—Cry4Ba. The interaction of these two individual toxins with target receptors on susceptible larval midgut cells is likely to be the critical determinant in their differential toxicity. Here, two full-length membrane-bound alkaline phosphatase (mALP) isoforms from Culex quinquefasciatus larvae, Cq-mALP1263and Cq-mALP1264, predicted to be GPI-linked was cloned and functionally expressed in Spodoptera frugiperda (Sf9) cells as 57- and 61-kDa membrane-bound proteins, respectively. Bioinformatics analysis disclosed that both Cq-mALP isoforms share significant sequence similarity to Aedes aegypti-mALP—a Cry4Ba toxin receptor. In cytotoxicity assays, Sf9 cells expressing Cq-mALP1264, but not Cq-mALP1263, showed remarkably greater susceptibility to Cry4Aa than Cry4Ba, while immunolocalization studies revealed that both toxins were capable of binding to each Cq-mALP expressed on the cell membrane surface. Molecular docking of the Cq-mALP1264-modeled structure with individual Cry4 toxins revealed that Cry4Aa could bind to Cq-mALP1264 primarily through particular residues on three surface-exposed loops in the receptor-binding domain—DII, including Thr<sup>512</sup>, Tyr<sup>513</sup> and Lys<sup>514</sup> in the β10-β11loop. Dissimilarly, Cry4Ba appeared to utilize only certain residues in its C-terminal domain—DIII to interact with such a Culex counterpart receptor. Ala-substitutions of selected β10-β11loop residues (T512A, Y513A and K514A) revealed that only the K514A mutant displayed a drastic decrease in biotoxicity against C. quinquefasciatus larvae. Further substitution of Lys<sup>514</sup> with Asp (K514D) revealed a further decrease in larval toxicity. Furthermore, in silico calculation of the binding affinity change (ΔΔG<inf>bind</inf>) in Cry4Aa-Cq-mALP1264 interactions upon these single-substitutions revealed that the K514D mutation displayed the largest ΔΔG<inf>bind</inf> value as compared to three other mutations, signifying an adverse impact of a negative charge at this critical receptor-binding position. Altogether, our present study has disclosed that these two related-Cry4 mosquito-active toxins conceivably exploited different domains in functional binding to the same Culex membrane-bound ALP isoform—Cq-mALP1264 for mediating differential toxicity against Culex target larvae.
  • Some of the metrics are blocked by your 
    Item type:Publication,
    Deep learning-based single-shot phase retrieval algorithm for surface plasmon resonance microscope based refractive index sensing application
    (2021-12-01)
    Thadson, Kitsada
    ;
    ;
    Pechprasarn, Suejit
    A deep learning algorithm for single-shot phase retrieval under a conventional microscope is proposed and investigated. The algorithm has been developed using the context aggregation network architecture; it requires a single input grayscale image to predict an output phase profile through deep learning-based pattern recognition. Surface plasmon resonance imaging has been employed as an example to demonstrate the capability of the deep learning-based method. The phase profiles of the surface plasmon resonance phenomena have been very well established and cover ranges of phase transitions from 0 to 2π rad. We demonstrate that deep learning can be developed and trained using simulated data. Experimental validation and a theoretical framework to characterize and quantify the performance of the deep learning-based phase retrieval method are reported. The proposed deep learning-based phase retrieval performance was verified through the shot noise model and Monte Carlo simulations. Refractive index sensing performance comparing the proposed deep learning algorithm and conventional surface plasmon resonance measurements are also discussed. Although the proposed phase retrieval-based algorithm cannot achieve a typical detection limit of 10<sup>–7</sup> to 10<sup>–8</sup> RIU for phase measurement in surface plasmon interferometer, the proposed artificial-intelligence-based approach can provide at least three times lower detection limit of 4.67 × 10<sup>–6</sup> RIU compared to conventional intensity measurement methods of 1.73 × 10<sup>–5</sup> RIU for the optical energy of 2500 pJ with no need for sophisticated optical interferometer instrumentation.
  • Some of the metrics are blocked by your 
    Item type:Publication,
    Bone mineral density screening system using CMOS-sensor X-ray detector
    (2021-11-01)
    Maneerat, Areerat
    ;
    ;
    This research concerns a design and construction of a bone mineral density (BMD) and bone mineral content (BMC) measurement system based on dual energy x-ray absorptiometry (DEXA). An indirect x-ray detector is designed by optical coupling CMOS sensor with image on the intensifying screen. A dedicated microcontroller x-ray apparatus is used as an x-ray source to cap-ture two energy level x-ray of middle phalanges bone of middle finger. The captured image is processed based on modified Beer-Lambert law to compute bone mineral density. Bone mineral content is also computed by determining the area of the phalanges bone using active contour. The designed bone mineral density (BMD) and bone mineral content (BMC) measurement system is low-cost and hence can be distributed at district hospital for screening purposes of Osteoporosis of the elderly. Compared with BMD measured from commercial model, BMD measurement of our system ac-quires linear relation with R<sup>2</sup> equals 0.969. The mean square error between the normalized BMD value and that of the commercial model is 0.0000981.
  • Some of the metrics are blocked by your 
    Item type:Publication,
    Feature-Based Classification of Mild Cognitive Impairment and Alzheimer’s Disease Based on Optical Coherence Tomographic Angiographic Image
    (2024-08-01) ;
    Maneerat, Areerat
    ;
    Trinavarat, Adisak
    ;
    Rattanabannakit, Chatchawan
    ;
    Morkphrom, Ekkaphop
    Alzheimer’s disease is a type of neurodegenerative disorder that is characterized by the progressive degeneration of brain cells, leading to cognitive decline and memory loss. It is the most common cause of dementia and affects millions of people worldwide. While there is currently no cure for Alzheimer’s disease, early detection and treatment can help to slow the progression of symptoms and improve quality of life. This research presents a diagnostic tool for classifying mild cognitive impairment and Alzheimer’s diseases using feature-based machine learning applied to optical coherence tomographic angiography images (OCT-A). Several features are extracted from the OCT-A image, including vessel density in five sectors, the area of the foveal avascular zone, retinal thickness, and novel features based on the histogram of the range-filtered OCT-A image. To ensure effectiveness for a diverse population, a large local database for our study was collected. The promising results of our study, with the best accuracy of 92.17,% will provide an efficient diagnostic tool for early detection of Alzheimer’s disease.