Investigation of rice blast resistant genes in Thai elite rice varieties (Oryza sativa L.) for improvement of broad-spectrum blast disease resistance variety

Abstract

The broad-spectrum blast disease resistance rice Huai variety (GS19567) has been reported to contain more than one blast resistance genes which one of them was mapped on chromosome 11. The blast resistance genes was investigated using gene specific primers in Huai and other 40 Thai elite rice varieties. The results obtained from polymerase chain reaction revealed that all tested varieties contained at least one blast resistant gene. The most frequent finding resistant gene was Pib, while Pib was missing in Huai variety. Therefore, in order to enhance broad spectrum blast disease resistance in a sustaining blast resistance variety, the resistance gene Pib is recommened to be used as candidate gene in breeding program.