Study on optimization for in vitro propagation of Dalbergia oliveri by plant tissue culture

Abstract

The experimental findings demonstrated that the highest proliferation frequency (100%), along with the maximum number of shoots per explant (3.33±0.52 shoots) and an average shoot length of 30.04±1.70 mm, were achieved in the Murashige and Skoog (MS) medium supplemented with 2.0 mg/l of 6-benzylaminopurine (BAP). Subsequently, upon reaching a height of 3 to 4 cm, the in vitro micro shoots were transferred to a ¼ MS medium enriched with 0.75 mg/l of indole-3-acetic acid (IAA). This particular regimen resulted in the highest number of roots per shootlet (3.50±0.58) with an average root length of 34.19±0.79 mm. The acclimatization phase of the plantlets to the soil was successful, demonstrating a commendable survival rate of 80%.