Identification of SRAP and AFLP molecular markers associated with fruit traits in santol (Sandoricum koetjape)

Abstract

The genetic diversity of santol (Sandoricum koetjape) using sequence-related amplified polymorphism (SRAP) and amplified fragment length polymorphism (AFLP) markers was investigated. Both molecular markers showed associations between fruit quality traits of economic importance, such as fruit weight and sweetness. The SRAP results revealed 128 amplified bands across nine primer pairs, indicating a polymorphism rate of 41.41%. Moreover, utilizing a genetic similarity coefficient of 0.85, santol cultivars were categorized into two major groups. Additionally, the AFLP analysis identified 171 amplified bands from seven primer pairs with a genetic similarity coefficient of 0.79. Consequently, santol cultivars were further classified into three distinct groups based on these findings. When combining the SRAP and AFLP results, a total of 299 amplified bands were analyzed, resulting in the separation of santol cultivars into four groups. These findings demonstrated that using SRAP and AFLP, as well as their combined results, could elucidate the genetic diversity within santol cultivars and may contribute to their classification.