Planosporangium thailandense sp. nov., isolated from soil from a Thai hot spring

Abstract

A novel filamentous bacterial strain, HSS8-18 T, was isolated from soil from a hot-spring pond in Krabi province, Thailand. The isolate produced single globose bodies and short, finger-like sporangia directly from the substrate mycelium. The cell wall of the isolate contained meso-diaminopimelic acid, glycine, arabinose and xylose. The characteristic phospholipids were diphosphatidylglycerol, phosphatidylglycerol, phosphatidylinositol, phosphatidylinositol mannosides, phosphatidylethanolamine and hydroxyl phosphatidylethanolamine. The predominant menaquinones were MK-10(H2), MK-9(H4) and MK-10(H4) and the predominant cellular fatty acids were anteiso-C 17: 0, iso-C 16: 0, iso-C 15: 0, iso-C 17: 0 and anteiso-C 15: 0. The G+C content of the genomic DNA was 72.0 mol%. 16S rRNA gene sequence analysis indicated that isolate HSS8-18 T belonged to the genus Planosporangium and shared the highest 16S rRNA gene sequence similarity with Planosporangium flavigriseum YIM 46034T (98.3 %) and P. mesophilum YIM 48875T (98.0 %). However, DNA-DNA hybridization results and some physiological and biochemical properties indicated that strain HSS8-18T could be readily distinguished from its closest phylogenetic relatives. Based on phenotypic and genotypic properties, isolate HSS8-18 T is judged to represent a novel species of the genus Planosporangium, for which the name Planosporangium thailandense sp. nov. is proposed. The type strain is strain HSS8-18 T (= BCC 41917 T = JCM 17129 T). © 2013 IUMS.