Effi ciency of lactic acid bacteria as the potent degradative microorganism to digest the total mixed ration in vitro

Abstract

The objective of this research was to investigate the effi ciency of lactic acid bacteria on in vitro digestibility of total mixed ration (TMR) containing pineapple plant silage 55%, rice straw 15%, cassava ship 5.6%, soybean meal 12.6%, palm kernel pulp l4.2%, rice bran 5.6%, premixed 0.15% and molasses 2%. Guinea grass (Panicum maximum) was used for isolation and identifi cation of microorganisms from silage. Treatments were used as TMR treated with lactic acid bacteria as follows: Treatment 1 – non treated; Treatment 2 – TMR treated with Lactobacillus pantarum; Treatment 3–TMR treated with Pediococcus pentosaceus; Treatment 4–TMR treated with Pediococcus acidilactici; Treatment 5 – TMR treated with L. pantarum + P. pentosaceus + P. acidilactici. The treated TMR were mixed and put in polyethylene bags and stored at ambient temperature for 7 days before analysis. The in vitro digestibility test used pepsin cellulase in vitro digestibility method. The results showed that there were signifi cant differences (P < 0.01) of the nutritive value of TMR by proximate analysis and fiber analysis which treated lactic acid bacteria. For the digestibility, results showed that the TMR treated with LAB using pepsin cellulase in vitro digestibility which applied LP, PP, PA and LPP could improve the digestibility of TMR, which signifi cantly increased in digestibility of TMR (P < 0.01). The rate of increase and decrease of digestibility and fi ber in TMR treated with LAB showed an increase in dry matter and a decrease in fi ber. Therefore, it was suggested that TMR treated with lactic acid bacteria of TMR may possible to develop as biological animal feed for ruminant.