Application of nano-particles derived from chaetomium elatum che01 to control pyricularia oryzae causing rice blast

Abstract

Pyricularia oryzae causing rice blast was isolated and proved for pathogenicity. Chaetomium elatum ChE01 was proved to be antagonized P. oryzae in bi-culture antagonistic test which averaged inhibition of 60.40 % within 15 days. Fungal metabolites from C. elatum ChE01 were extracted and tested to inhibit P. oryzae. Results showed that crude ethyl acetate expressed antifungal activity against P. oryzae which the effective dose 50 (ED 50 ) was 231 ppm., followed by crude methanol and crude hexane which the ED 50 were 460 and 2,122 ppm. respectively. It was shown that nano-CCE gave the highest inhibition P. oryzae which the ED 50 was 8.25 ppm, and followed by nano-CEM and nano-CEH which the ED 50 values were 11.21 and 65.52 ppm, respectively. Further research findings are investigated in pot and field experiments.