Nano-particles from Cheatomium brasiliense against brown spot of rice

Abstract

Chaetomium brasiliense was used in this study to control rice brown spot disease pathogen, Drechslera oryzae. The result of bi-culture test showed that Ch. brasiliense gave the hightest percentage of growth inhibition at 26.38% and had the highest spore inhibition at 23.81%. In crude extracts test, it was found that crude methanol extract obtained from Ch. brasiliense at 1000 ppm showed the highest inhibitory effect on colony growth and spore production. Ch. brasiliense gave a growth inhibition and spore inhibition rates at 83.50 and 99.78% respectively. The effective dose (ED 50 ) on growth and spore inhibition of Ch. brasiliense was 80.54 and 0.35 µg/ml, respectively. It was also found that Nano-particles obtained from crude methanol extract of Ch. brasiliense (nano CBM) at 10 ppm had the best inhibitory effect in terms of growth and spore inhibition. Nano CBM can inhibit the growth at 70.00% and spore production at 79.92%. The ED 50 values for spore inhibition of Ch. brasiliense was 2.86 µg/ml.