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Thermal degradation kinetics and purification of C-phycocyanin from thermophilic and mesophilic cyanobacteria
Author(s)
Chittapun, Supenya
Suwanmanee, Kattiya
Kongsinkaew, Chatchol
Chisti, Yusuf
Charoenrat, Theppanya
Date Issued
February 1, 2025
Type
Article
Abstract
The natural blue colorant C-phycocyanin (C-PC) has many potential applications but its poor heat stability limits its commercial use. This study compares the production and thermal stability of C-PC from two cyanobacteria: the thermophilic Thermosynechococcus sp. TUBT-T01 and the mesophilic Synechococcus cedrorum TISTR8589. Thermosynechococcus sp. produced nearly 1.9-fold more C-PC than S. cedrorum. Batch adsorption using a chromatographic cationic ion exchange resin (Streamline Direct HST1) was used to effectively purify the C-PC. The equilibrium adsorption capacity (Qeq) of the resin for C-PC was the highest at pH 5. At this pH, the Qeq for the thermophilic C-PC was 5.5 ± 0.1 mg mL⁻¹, whereas for the mesophilic C-PC it was 1.5 ± 0.2 mg mL⁻¹. Purification increased the concentration of the thermophilic C-PC by 5.9-fold, and that of mesophilic C-PC by 4.2-fold. The purity ratios of the final products from the two cyanobacteria were similar at ∼2.2. At 60 °C and pH 7, the C-PC of Thermosynechococcus sp. had ∼12-times longer half-life than the mesophilic C-PC; however, the productivity of the thermophilic C-PC was comparatively low because of a low biomass productivity of Thermosynechococcus sp.
Citation
Journal of Biotechnology, 398, 76-86, 2025