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    Top-spray fluidization coating of paddy rice with zinc oxide nanoparticles to reduce infection from Aspergillus sp
    (2020-10-01) ;
    Junka, Nittaya
    The aim of this study was to reduce infection of paddy rice from Aspergillus sp. by coating with zinc oxide nanoparticles (ZnO NPs) solution in a top-spray fluidization coater, while maintaining the important qualities. The nozzle height and the atomized pressure affected the final moisture content of the coated paddy rice. When the final moisture content decreased lower than 13.0% (d.b.), this resulted in a drastic decrease in the husking percentage. Analysis of the crystallinity structure using X-ray diffractometry could indicate the amount of ZnO NPs coated to the paddy rice surface under appropriate coating conditions. The atomization of the ZnO NPs solution through a nozzle at 35 cm height, a pressure of 3.5 bar, and a concentration of 7.7 g/kg were the optimal conditions for complete inhibition of fungal growth while maintaining the important qualities of paddy rice. Practical applications: Fungi can infect paddy rice at any stage from the planting process through growth, harvest, and storage, and cause low quality produce. This research studied the application of a top-spray fluidization technique and the benefits of coating paddy rice with ZnO NPs to inhibit Aspergillus sp. during storage. The application of a top-spray fluidization technique can maintain the final moisture content of paddy rice without exceeding the equilibrium, rendering the paddy rice suitable for packing for transport, or storage directly after the coating procedure. The data obtained from this research can determine the most appropriate coating conditions and this coating system can be employed in rice community enterprises, cooperatives, or factories.
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    Antifungal copper nanocomposite-rubber compound for tree wound dressings
    (2024-12-15)
    Noppradit, Benjaporn
    ;
    Uthaipan, Nattapon
    ;
    Klinnawee, Lompong
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    ;
    Phengdaam, Apichat
    This study combines copper nanoparticles (CuNPs) with a rubber compound to develop a tree wound dressing that reduces tree mortality from infections, utilizing broad-spectrum antifungal and adhesive properties of composite material. Phytotoxicities of CuNPs with an average size of 6 nm were significantly lower than those of Cu ions, evaluated by the germination index using lettuce seeds and membrane integrity index using stems of rubber seedling. After that, the synthesized CuNPs were then loaded into a natural rubber (NR) latex compound at concentrations ranging from 0 to 90 mg/L to produce a CuNP composite adhesive. The shear strength, representing adhesion on wood, was evaluated using lap shear testing, revealing a 50 % reduction in maximum force at the highest CuNP loading. This loss of shear strength was attributed to interference by copper ions in the vulcanization of the NR latex compound, as indicated by FT-IR spectroscopy. After that, the CuNP composite adhesive was formed into a film to test antifungal properties against Colletotrichum spp. Based on the in vitro plate inhibition zone assay, 10 mg/L of CuNPs inhibited the growth of Colletotrichum spp. The size of inhibited zone was significantly increased by 150 and 300 % when concentrations of CuNPs were 70 and 90 mg/L, respectively. This research provides the first evidence of the low phytotoxicity of CuNPs compared to copper ions at equivalent concentrations and demonstrates the efficacy of CuNP composites with NR compounds as tree wound dressings.
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    In vitro fungicidal effect of chitosan with different molecular weights on fungicide-resistant Phytophthora fruit rot on durian from the export market
    (2018-08-30)
    Fruit rots of durian were collected from the export market in Chumphon province, Thailand. The causal organism, Phytophthora spp. was isolated on PAR(PH)-V8 selective medium by tissue transplanting technique. Resistance to dimethomorph, fosetyl-Al, metalaxyl and mancozeb was detected from the mycelial growth on V8 agar amended with fungicide. Multiple fungicide resistant (Mu-F<sup>HR</sup>) and sensitive (Mu-F<sup>S</sup>) isolates are used to study the fungicidal effect of chitosan with different molecular weights. The low, medium and high molecular weight chitosan at 0 (control), 500, 1,000 and 2,000 ppm were evaluated for the development of Mu-FHR and Mu-FS isolates on mycelial growth by poisoned food technique and on sporangium production and zoospores release by culture disc technique. The results indicated that the fungicidal effect of three types of chitosan strengthened as the concentration of chitosan increased for mycelial and sporangium inhibition. However, all chitosan treatments were not significant in zoospores release.
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    Sensitivity tests of dimethomorph, ethaboxam and etridiazole on Phytophthora palmivora causing stem rot and leaf blight of durian in eastern Thailand
    (2023-01-01)
    Tongsri, Veeranee
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    Nianwichai, Patcharin
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    Sichai, Kamonwan
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    Songkumarn, Pattavipha
    ;
    Suttiviriya, Pavinee
    Importance of the work: Phytophthora palmivora is a major pathogen on durians and has been found to be resistant to metalaxyl. Objectives: To investigate the sensitivity of P. palmivora to alternative fungicides and evaluate its fungicide efficacy for controlling durian diseases. Materials & Methods: The isolates of P. palmivora were evaluated for their fungicide sensitivity to dimethomorph, ethaboxam and etridiazole on the culture medium against mycelial growth, sporangium production and germination. The representative isolates were determined for fungicide sensitivity on durian leaf tissue. The fungicides with low values for the concentration that inhibited the mycelial growth of the isolate by 50% (EC<inf>50</inf>) were used for durian disease control. Results: The mycelial growth of all P. palmivora isolates was sensitive to dimethomorph, ethaboxam and etridiazole, with mean (± SD) EC<inf>50</inf> values of 0.233 ± 0.116 parts per million (ppm), 0.007 ± 0.002 ppm and 2.945 ± 1.464 ppm, respectively. Sporangium production and germination were also sensitive to all fungicides. The mean EC<inf>50</inf> values on durian leaf tissue were 0.696 ppm for dimethomorph, 0.089 ppm for ethaboxam and 0.766 ppm for etridiazole. Dimethomorph (100 ppm) and ethaboxam (50 ppm) completely reduced disease development on detached leaves; however, etridiazole (50 ppm) showed maximum control of the disease by 55.6%. Only ethaboxam (208 ppm) extremely suppressed the disease in a seedling experiment (92.3%), while dimethomorph (500 ppm) produced much less disease control (27%). Etridiazole (240 ppm) could not control the disease in seedling conditions. Main finding: Ethaboxam had the potential to control durian disease caused by P. palmivora in the eastern growing areas of Thailand. Repeat fungicide application should be discussed regarding dimethomorph and etridiazole.
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    Isolates of Colletotrichum truncatum with Resistance to Multiple Fungicides from Soybean in Northern Thailand
    (2023-09-01)
    Poti, Teeranai
    ;
    Thitla, Tanapol
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    Imaiam, Naphatsawan
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    Arunothayanan, Hatthaya
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    Doungsa-Ard, Chanintorn
    In Thailand, four systemic fungicides-carbendazim (Car), azoxystrobin (Azo), difenoconazole (Dif), and penthiopyrad (Pen)-are commonly used to control soybean anthracnose caused by Colletotrichum truncatum; however, the pathogen has developed resistance. From 2019 to 2020, fungicide resistance in C. truncatum from fields in Chiang Rai and Chiang Mai was monitored. In tests of 85 C. truncatum isolates for resistance to multiple fungicides, 15.3% were CarRAzoR, 34.1% were triple resistant (CarRAzoRDifR or CarRAzoRPenR), and 50.6% were CarRAzoRDifRPenR. Surprisingly, all isolates tested had lost their sensitivity to one or more of the fungicides tested. The carbendazim-resistant isolates carried a point mutation in the b-tubulin gene at codon 198 (E198A) or 200 (F200Y), and all azoxystrobin-resistant isolates had a mutation in the cytochrome b gene at codon 143 (G143A) or 129 (F129L). Moreover, a novel mutation at codon 208 (S208Y) in the gene encoding succinate dehydrogenase subunit B was detected in all of the isolates highly resistant to penthiopyrad. No mutation linked with difenoconazole resistance was detected in the genes encoding cytochrome P450 sterol 14a-demethylase. To the best of our knowledge, this is the first report of C. truncatum isolates resistant to multiple fungicides and serves as a warning to take measures to prevent the occurrence and distribution of these multiple-fungicide-resistant populations in soybean fields.
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    Cross-Resistance to Benzimidazole Group and Mancozeb Fungicides in Colletotrichum spp. Causing Anthracnose Disease
    (2022-10-01) ;
    Somnuek, Suriyasit
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    Udompongsuk, Mongkutkarn
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    Prasom, Panisa
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    Jaenaksorn, Tanimnun
    Anthracnose is considered to be one of the most serious diseases for major crops cultivated in Thailand. Although chemical fungicides and newer chemicals have been recommended and used for disease management, it is still difficult to control. Thus, the monitoring of fungicide resistance development is required and very important for the success of disease control strategies. In this study, a total of 34 isolates of Colletotrichum spp. were successfully collected from anthracnose diseased plants in central Thailand. Of these isolates, 16 were collected from mango, and 2 from crinum lily or orchid and were morphologically identified as C. gloeosporioides, while the other 16 isolates from chili were morphologically identified as C. capsica. Then, all isolates were evaluated in vitro against 3 fungicides to determine their sensitivity. The results showed that 25 isolates were cross-resistant to both benomyl and carbendazim, as the 50% effective concentration (EC<inf>50</inf>) of each fungicide was >100 mg L<sup>-1</sup>. All isolates were resistant to mancozeb. Nine out of 16 isolates from mango anthracnose were confirmed as C. gloeosporioides by the sequence of internal transcribed spacer (ITS) and 5. 8S regions of rDNA. Eight cross-resistant isolates were not controlled by the treatment with 100 mg L<sup>-1</sup> of benomyl or carbendazim on ‘Nam Dok Mai’ mango fruits, but sensitive isolate was better controlled at 79.33% and 85.11%, respectively. Our results indicated that cross-resistant strains are present in the populations of anthracnose fungi in central Thailand.
      1
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    EFFECT OF ZINC OXIDE NANOPARTICLES AGAINST PHYTOPHTHORA SPP. CAUSING DURIAN DISEASES IN VITRO
    (2024-01-01) ; ;
    Pantakan, Sansanee
    The efficiency of zinc oxide nanoparticles (ZnO-NPs) in inhibiting Phytophthora spp. growth in both durian fruits and stems is demonstrated. The size of ZnO-NPs used was in range of 25-50 nm in diameter. Diseased durian sample were collected from a durian orchard located in southern of Thailand. The pathogen of diseased durian was isolated for subsequent morphological identification. Three distinct strains of Phytophthora spp. (designated as PHY41, PHY45, PHY49) were successfully isolated and were found in colonies with rings and stellate pattern, ovoid, limoniform sporangium with semi-papillate or papillate, and formation chlamydospores. All isolates were exhibiting 100% pathogenicity on durian, as indicated by the disease incidence (DI). The concentration of ZnO-NPs played a crucial role in suppressing the growth of Phytophthora spp. in all isolates. Higher concentration of ZnO-NPs resulted in increased inhibition of growth. ZnO-NPs at the concentration of 2000 µg/mL effectively suppressed the mycelial growth of all fungi isolates at 57.76-69.84%. Additionally, ZnO-NPs at the concentration of 500 µg/mL completely inhibited the sporulation only in the PHY49 strains. Furthermore, ZnO-NPs concentration ranging from 1000-2000 µg/mL resulted in completed inhibition in sporulation in all Phytophthora spp. isolates. The result demonstrated that ZnO-NPs at all concentrations effectively delayed the germination of Phytophthora spp.
      2
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    Synergistic effect of salicylhydroxamic acid in quinone outside inhibitor fungicides and its sensitivity on fruit rot pathogens
    The synergistic effect of treatment with both salicylhydroxamic acid (SHAM) and treatment with azoxystrobin (AZ), trifloxystrobin (TF), and pyraclostrobin (PR) which are present in Ool (quinone outside inhibitor) on fruit rot pathogens was investigated. Twenty-five fungal pathogen isolates were collected from chili, mango, and durian fruit rot. Ten isolates of Phytophthora palmivora were obtained from durian fruits and 15 Colletotrichum spp. were obtained from chili and mango fruits. The efficiency of SHAM interaction with AZ, TF, and PR at various concentrations to mycelial growth was statistically defined by a synergy factor (SF). A value of a SF above 1 represented the synergism of SHAM and fungicide in QoI to inhibit fungi respiration. The fungicide sensitivity was evaluated with the classification of the levels of sensitivity based on 50% effective concentrations (EC<inf>50</inf>). All tested isolates were susceptible to QoI fungicides at EC<inf>50</inf> lower than 10 mg L<sup>-1</sup>. Only one isolate of Colletotrichum sp. from chili (CC_P003) was intermediately resistant to AZ at 16.52 mg L<sup>-1</sup> EC<inf>50</inf>.
      1
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    In vitro assessment of crude extract from Gomphrena weed (Gomphrena celosioides Mart.) for control of plant pathogenic fungi causing chili diseases
    (2023-03-01)
    Apithanasakulngeon, P.
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    Somnuek, S.
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    ;
    Jaenaksorn, T.
    The phytochemical screening of both the flowers and other aerial parts of the Gomphrena weed extract (Gomphrena celosioides Mart.) presented alkaloids, flavonoids, saponins, phenolics/tannins, and terpenoids. The extracts from both parts at 80, 160, and 240 mg/ml were significantly inhibited the mycelial growth of C. gloeosporioides and Fusarium sp. compared to inoculated control by paper disc diffusion. The extract from both parts of the Gomphrena weed at the highest concentration (160 mg/ml) significantly showed the greatest inhibitory effect on mycelial growth of Sclerotium sp. (100 percent), followed by C. gloeosporioides (82.5 percent), Cercospora sp. (41.16 percent), and Fusarium sp. (24.41 percent) when tested using the poisoned food technique. The spore germination test revealed both parts of the weed extract yielded concentrations which were completely inhibited (100 percent) on the spore germination of C. capsici, C. gloeosporioides, and Fusarium sp.
      2
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    Low-Cost Activated Carbon for Methylene Blue Adsorption Using Inert Gas-Free Carbonization and Microwave Activation
    (2025-01-01) ; ;
    Choola-Aied, Orasa
    -This study investigated the preparation of activated carbon from spent coffee grounds for methylene blue adsorption using inert gas-free carbonization and microwave activation. Spent coffee grounds were obtained from a local coffee shop in Pathio, Chumphon, Thailand. The coffee grounds were pretreated with 1M H<inf>2</inf>SO<inf>4</inf> before undergoing carbonization at 600 ºC for 3 h in a limited-oxygen atmosphere. The obtained carbon materials were chemically activated with zinc chloride (ZnCl<inf>2</inf>) and physically activated using a microwave-assisted method at 300 W for 180 seconds. The adsorption of methylene blue was evaluated using a batch method. Methylene blue adsorption contact time, effect of pH, and initial concentration were investigated. The maximum adsorption capacity of the activated carbon for methylene blue removal was found to be 29.24 mg/g. The prepared activated carbon exhibited a BET surface area of 70.08 m<sup>2</sup>/g and reached equilibrium in 120 min at the optimum pH of 9. The adsorption isotherm was consistent with the Freundlich model, while the adsorption kinetics followed a pseudo-second-order model. The findings indicate that activated carbon is a cost-effective and efficient adsorbent for methylene blue removal from aqueous solutions.
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