Micromonospora oryzae sp. Nov., isolated from roots of upland rice

An actinomycete strain, designated CP2R9-1^T, was isolated from root internal tissues of upland rice (Oryza sativa). Based on a polyphasic approach, strain CP2R9-1^T was characterized as a member of the genus Micromonospora. meso- Diaminopimelic acid and 3-OH-diaminopimelic acid were present in the cell-wall peptidoglycan. The polar lipids were diphosphatidylglycerol, phosphatidylethanolamine, phosphatidylglycerol, phosphatidylinositol, phosphatidylinositol mannosides, two unidentified phospholipids and four unidentified polar lipids. Predominant menaquinones were MK-9(H4), MK-9(H6) and MK-10(H4). Whole-cell sugars consisted of ribose, xylose, arabinose and glucose. Phylogenetic analysis of the nearly complete 16S rRNA gene sequence suggested that strain CP2R9-1^T was closely related to Micromonospora haikouensis 232617^T (99.32% similarity), Micromonospora carbonacea DSM 43168^T (99.18%) and Micromonospora krabiensis MA-2^T (99.16%). Strain CP2R9-1^T was distinct from its closest relatives based on low levels of DNA–DNA relatedness (21.3¡0.1– 41.7¡0.7%) and phenotypic differences. The results presented in this study showed that strain CP2R9-1^T represents a novel species of the genus Micromonospora, for which the name Micromonospora oryzae sp. nov. is proposed. The type strain is CP2R9-1^T (5BCC 67266T5NBRC 110007^T).

Citation

International Journal of Systematic and Evolutionary Microbiology, 65(11), 3818-3823, 2015