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Pseudoxanthomonas helianthi sp. Nov., isolated from roots of Jerusalem artichoke (Helianthus tuberosus)
Date Issued
December 1, 2016
Type
Article
Abstract
A bacterium designated as strain roo10T was isolated from roots of Jerusalem artichoke (Helianthus tuberosus). Cells were Gram-stain-negative and non-motile rods. The phylogenetic analysis of the 16S rRNA gene indicated that it represented a member of the genus Pseudoxanthomonas, and its close relatives included Pseudoxanthomonas kalamensis JA40T (97.8% 16S rRNA gene sequence similarity), Pseudoxanthomonas sangjuensis 5GH38-5T (97.7%) and Pseudoxanthomonas daejeonensis TR6-08T (97.1%). Growth of roo10T occurred at pH 7-9. The temperature for growth ranged from 20 to 37 °C. Tolerance to NaCl was observed from 0.005 to 5% (w/v) concentration. Predominant fatty acids were iso-C15:0 (23.5%), iso-C16:0 (18.9%) and anteiso-C15:0 (11.5%). Diphosphatidylglycerol, phosphatidylglycerol, phosphatidylethanolamine and phosphatidyl-N-methylethanolamine were the major polar lipids. The predominant quinone was ubiquinone 8 (Q-8). The DNA G+C content was 65.7 mol% [from melting temperature (Tm)]. Comparison of phenotypic and chemotaxonomic characteristics indicated that roo10T was distinguishable from its close relatives. Additionally, the DNA-DNA relatedness levels between roo10T and P. kalamensis DSM 18571T (22±0.5%), P. sangjuensis 5GH38-5T (21±0.2%) and P. daejeonensis DSM 17801T (3±1%) were lower than 70%. These results indicated that roo10T represented a novel species of the genus Pseudoxanthomonas, for which the name Pseudoxanthomonas helianthi sp. nov. is proposed. The type strain is roo10T (=BCC 70700T=NBRC 110414T).
Citation
International Journal of Systematic and Evolutionary Microbiology, 66(12), 5034-5038, 2016