Evaluation of rapid detection system for Escherichia coli in water samples

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dc.contributor.authorPipitsombat, Chanikarn
dc.contributor.authorPhasuk, Kanchana
dc.contributor.authorSuwan, Supakorn
dc.contributor.authorPiyawattanametha, Wibool
dc.date.accessioned2026-08-06T10:16:14Z
dc.date.available2026-08-06T10:16:14Z
dc.date.issued2017-02-21
dc.description.abstractWe demonstrated a fast detection platform for Escherichia coli (E. coli) in water. The system is based on a fluorescence detection technique by determining enzyme β-D-glucuronidase (GUD) activity and relating that activity to E. coli concentration. We found the portable E. coli detection platform is able to detect both the fluorescence 4-MU signal from the reaction of GUD assay from 0.01-1 μg/ml and to enumerate the number of E. coli in the range of 10<sup>5</sup>-10<sup>7</sup> CFU/ml. The required incubation time is in between 20-240 min. In addition, we can estimate the maximum of incubation time for detecting minimum of E. coli 1 CFU/ml from the equation to be 576 mins. Our preliminary study demonstrates that the system can be used to enumerate the number of E. coli in water on-site and requires less detection time than traditional E. coli methods.
dc.identifier.citationBmeicon 2016 9th Biomedical Engineering International Conference, 2017
dc.identifier.doi10.1109/BMEiCON.2016.7859595
dc.identifier.other2-s2.0-85015891412
dc.identifier.urihttps://dspace.kmitl.ac.th/handle/123456789/7526
dc.sourceBmeicon 2016 9th Biomedical Engineering International Conference
dc.subjectb-D-glucuronidase (GUD)
dc.subjectdrinking water
dc.subjectEscherichia coli
dc.subjectfluorescence
dc.subjectportable detector
dc.titleEvaluation of rapid detection system for Escherichia coli in water samples
dc.typeConference Paper

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