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    Item type:Publication,
    Predicting and explaining high dead-on-arrival outcomes in meat-type ducks using deep learning: A path to improved welfare management
    (2025-09-01)
    Jainonthee, Chalita
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    Sanwisate, Phutsadee
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    ; ;
    Pichpol, Duangporn
    Dead-on-arrival (DOA) rates are a critical welfare and economic concern in poultry production, reflecting the cumulative impact of handling, transport, and lairage conditions on bird mortality. Compared to broilers and layers, meat-type ducks have received less attention in DOA research, despite their distinct physiological responses to preslaughter stressors and increasing relevance in commercial poultry production. Although machine learning models have been widely applied for DOA prediction, their limited transparency can hinder practical application in real-world settings. This study analyzed 8220 truckload entries of meat-type ducks recorded between 2022 and 2023, with the objective of developing an explainable deep learning model to predict high DOA outcomes using preslaughter management and environmental data. Deep learning models, owing to their complex architecture, offer superior predictive capacity and can capture nonlinear interactions in high-dimensional datasets. To enhance model interpretability and support practical application, SHapley Additive exPlanations (SHAP) was applied to identify the most influential predictors of DOA classification. The final model demonstrated strong classification performance, with an accuracy of 80.29 %, precision of 79.25 %, recall of 80.29 %, F1-score of 79.66 %, and an AUC-ROC of 76.03 %. Key predictors of high DOA included duck head count, lairage temperature, duck age, and transport duration. Notably, a higher number of ducks per truckload was strongly associated with elevated DOA risk (i.e., truckloads classified in the high DOA group), along with lairage temperatures and duck ages below the respective medians. Additionally, shorter transport durations were linked to increased mortality, highlighting the complex interplay of preslaughter stressors. By leveraging SHAP analysis, this study provided both global and local interpretability, ensuring that model outputs were not only accurate but also explainable. These findings support precision-driven preslaughter interventions, enabling industry stakeholders to optimize handling, transport, and lairage practices to reduce mortality rates and enhance duck welfare.
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    Item type:Publication,
    Field-based and molecular evaluation of anthelmintic resistance in gastrointestinal strongyle nematodes of meat goats in Southern Thailand
    (2025-08-01) ;
    Sansamur, Chalutwan
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    Klong-Klaew, Tunwadee
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    Mektrirat, Raktham
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    Kaewthamasorn, Morakot
    Background and Aim: Gastrointestinal nematodes (GINs) such as Haemonchus contortus and Trichostrongylus colubriformis are major health threats in goats, exacerbated by growing anthelmintic resistance (AR). Despite the widespread use of albendazole and ivermectin in Nakhon Si Thammarat, Southern Thailand, data on AR status in goats remain scarce. This study aimed to evaluate the resistance of GINs to albendazole and ivermectin using fecal egg count reduction (FECR) tests and to determine benzimidazole (BZ) resistance through molecular detection of the F200Y mutation in the β-tubulin isotype 1 gene. Materials and Methods: A total of 192 meat goats from six farms were randomly divided into four groups: untreated control, albendazole-treated, ivermectin-treated, and combination-treated. FECR was assessed on day 14 post-treatment. Larval cultures and semi-nested polymerase chain reaction (PCR) were used to identify nematode genera. BZ resistance was determined through allele-specific PCR on H. contortus and T. colubriformis third-stage larvae, pre- and post-albendazole treatment. Results: FECR revealed confirmed resistance (FECR < 95%) to albendazole (−35.48%–62.5%), ivermectin (−2.41%–51.47%), and their combination (−25%–48.36%) across all farms. Haemonchus and Trichostrongylus were the predominant genera post-treatment. Molecular analysis showed high pre-treatment frequencies of the F200Y resistance allele in H. contortus (75.0%–80.6%) and T. colubriformis (88.6%–100%), which reached 100% post-treatment. Susceptible genotypes were entirely eliminated following albendazole treatment. Conclusion: This is the first comprehensive study confirming widespread AR to albendazole and ivermectin in meat goats in southern Thailand. The fixation of the BZ resistance allele in both nematode species highlights the urgency for revising current deworming practices. Immediate adoption of integrated parasite management strategies, including drug rotation, targeted selective treatment, and exploration of alternative anthelmintics, is critical to mitigate economic losses and protect public health.
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    Item type:Publication,
    Seasonal Infective Dynamics and Risk Factors Associated with Prevalence of Zoonotic Gastrointestinal Parasites from Meat Goats in Southern Thailand
    (2025-07-01) ;
    Sansamur, Chalutwan
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    Klong-Klaew, Tunwadee
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    Kaewthamasorn, Morakot
    ;
    Fungwithaya, Punpichaya
    Gastrointestinal (GI) parasites not only significantly impact goat health and productivity but can also affect human health due to the zoonotic potential of some species. This study investigates the prevalence of internal parasites within the tropical monsoon ecosystem of southern Thailand, focusing on both phenotypic and molecular characteristics of the parasites and identifying associated risk factors in caprine farming systems. A total of 276 meat goats from Nakhon Si Thammarat province were examined, indicating an overall GI parasite prevalence of 88.8% (245/276), with strongyles and Eimeria spp. identified as the dominant parasites. In addition, mixed parasitic infections were observed in 72.2% of cases, whereas single infections comprised 27.8%. Strongyle-positive fecal samples were cultured and genetically sequenced, revealing the presence of Haemonchus contortus, Trichostrongylus colubriformis, and Oesophagostomum asperum. For associated risk factors, gender and grazing with other herds significantly impacted overall GI parasitic infections, while the gender, breed, and packed cell volume (PCV) affected the strongyle infection. A correlation analysis revealed a substantial relationship between strongyle egg per gram (EPG) counts and clinical parameters, indicating that monitoring animals with low body condition scores (BCS) and high Faffa Malan Chart (FAMACHA) scores could be an effective strategy for controlling strongyle infections. These findings highlight the importance of continued research and effective farm management practices to address strongyle infections in meat goats, improving their health and agricultural productivity in tropical regions. Moreover, the detection of four zoonotic parasites (Giardia spp., H. contortus, T. colubriformis, and Fasciola spp.) indicates the necessity for the routine surveillance and monitoring of zoonotic parasites in goats to mitigate potential human health risks.
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    Item type:Publication,
    Data-driven insights into pre-slaughter mortality: Machine learning for predicting high dead on arrival in meat-type ducks
    (2025-01-01)
    Jainonthee, Chalita
    ;
    Sanwisate, Phutsadee
    ;
    ; ;
    Mektrirat, Raktham
    Dead on arrival (DOA) refers to animals, particularly poultry, that die during the pre-slaughter phase. Elevated rates of DOA frequently signify substandard welfare conditions and might stem from multiple causes, resulting in diminished productivity and economic losses. This study included 18,643 truckload entries from 45 farms, encompassing a total of 23,191,809 meat-type ducks sent to a single slaughterhouse in Eastern Thailand between January 2019 and December 2023. The objective of this study was twofold: first, to classify high DOA rates (≥ 0.15%) using several predictors, including season, period of the day, number of ducks per truckload, distance, duration of transportation, age, average body weight, lairage time, and temperature at the lairage area. This classification was performed using machine learning (ML) algorithms such as Least Absolute Shrinkage and Selection Operator (LASSO), Support Vector Machine (SVM), Decision Tree (DT), Random Forests (RF), and Extreme Gradient Boosting (XGBoost). Additionally, several data-sampling techniques, including oversampling, undersampling, Random Over-Sampling Examples (ROSE), and Synthetic Minority Over-sampling Technique (SMOTE), were utilized to address the issue of imbalanced data. Second, to analyze variable importance contributing to the predictive outcomes. The descriptive analysis revealed a mean DOA percentage of 0.14% (range: 0 to 22.46%, SD = 0.49). The results of the high DOA classification indicated that among all models, XGBoost-Up, XGBoost-Down, and RF-Down were the top three models, achieving the highest overall scores in evaluation metrics including Area Under the ROC Curve (AUC), sensitivity, precision, and F1-score. The primary factors contributing to the high predictive performance of the models were the number of ducks per truckload, temperature at the lairage area, and average body weight. Additionally, the duration and distance of transportation, as well as the period of transportation, were secondary factors contributing to the outcome. These factors should be further investigated to minimize losses during the pre-slaughter phase in meat-type ducks. Additionally, considering these factors when managing transportation can help create conditions that reduce duck deaths.
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    Item type:Publication,
    Utilizing liposomal encapsulation approach to address nephrotoxic challenges of colistimethate sodium through a preclinical study
    (2023-01-01)
    Mektrirat, Raktham
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    Paengjun, Noppanut
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    Chongrattanameteekul, Peerawit
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    Umsumarng, Sonthaya
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    Cheunsri, Suppara
    The use of Colistin, a last-resort antimicrobial drug, carries the risk of acute kidney injury. The objective of the study was to assess the effectiveness of colistin-encapsulated liposomes (CL) in reducing nephrotoxicity. Additionally, a liposomal preparation of colistimethate sodium was formulated using the reverse phase evaporation method with a 3:1 ratio of phospholipids to cholesterol. The liposomal properties were evaluated using scanning electron microscopy, photon correlation spectroscopy, and release kinetic assay. The killing kinetics of the formulations on embryonic kidney cells were assessed using in vitro MTT reduction assay. The nephrotoxicity of CL and colistimethate sodium solution (CS) was evaluated in vivo by administering a dose of 20 mg/kg to rats every 12 h for 3 days, with a negative control group receiving a 0.9% saline solution (NSS). The study results revealed that monodisperses of CL showed a smooth surface and distinct boundaries, with an average size of 151.50 ± 0.46 nm and a narrow size distribution of 0.25 ± 0.01. The liposomal particles showed high entrapment efficiency of 96.45% ± 0.41%, with a ζ-potential of −60.80 ± 1.01 mV and a release rate of 50% of colistimethate sodium within the first 480 min. The CL induced nephrocytotoxicity in a concentration- and time-dependent manner. However, CS had notably lower IC<inf>50</inf> values compared to its liposome preparations at 48 and 72 h (p < 0.05). In vivo study results show that serum levels of symmetric dimethylarginine (SDMA) and total white blood cell count (WBC) were significantly lower in the CL group (SDMA = 8.33 ± 1.70 μg/dL; WBC = 7.29 ± 0.99 log<inf>10</inf> cells/mL) compared to the CS group (SDMA = 15.00 ± 1.63 μg/dL; WBC = 9.73 ± 0.51 log<inf>10</inf> cells/mL). Our study findings enhance the understanding of the safety profile of CL and its potential to improve patient outcomes through the use of liposomal colistin medication. Additional clinical studies are necessary to establish the optimal safety regiment in humans.