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Author: search.filters.author.Kanokmedhakul, S.Subject: search.filters.subject.Nano-particles

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    Nano-particles from Chaetomium brasiliense to control Phytophthora palmivora caused root rot disease in durian var Montong
    (2018-12-01)
    Tongon, R.
    ;
    Soytong, K.
    ;
    Kanokmedhakul, S.
    ;
    Kanokmedhakul, K.
    The antagonistic fungus, Chaetomium brasiliense was tested to control Phytophthora palmivora causing rot disease of Durian (Durio zibithenus L.) var Montong by crude extracts and Nano-particles derived from Ch. brasiliense. Crude extracts of antagonistic fungus was tested for antifungal biological activities. The crude extracts from antagonistic fungus with hexane, ethyl acetate and methanol were tested against P. palmivora. Crude ethyl acetate from Ch. brasiliense gave significantly against P. palmivora which the ED <inf>50</inf> values was 17.46 µg/ml. Testing Nano-particles were tested for antifungal activities. The results showed nano - particles from Ch. brasiliense gave effectively significantly inhibition of colony growth and spore production which the ED <inf>50</inf> values were 1.08 µg/ml, and 8.68 µg/ml respectively. Application of Nano - particles to control the P. palmivora causing root rot disease of durian in pot experiment was successfully done. The results showed nano-particles from Ch. brasiliense reduced the root rot disease on durain of 40%. The nano-particles from Ch. brasilense gave significiantly high plant growth which were 79.5 cm when compared to the non-trated control.
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    Nano-particles from Chaetomium lucknowense to inhibit rice blast pathogen causded by Pyricularia oryzae in pot experiment
    (2018-12-01)
    Song, J. J.
    ;
    Soytong, K.
    ;
    Kanokmedhakul, S.
    ;
    Kanokmedhakul, K.
    Nano-particles derived from Chaetomium lucknowense proved to be antagonized Pyricularia oryzae causing rice blast disease var. RD57. Result showed that nano-ECL, nano-MCL and nano-HCL expressed antifungal activities against P. oryzae (rice blast disease) at the ED <inf>50</inf> values were 82, 114, 181ppm, respectively. In pot experiment, the nano-CL gave significantly better to control rice blast than the chemical fungicide (Tricyclazole) in rice var. RD57. Rice blast disease showed that nano-CL gave the highest reduction of 54 %, when compared to the chemical fungicide that the disease decreased 29.26 %. Application of nano-CL gave the highest plant strands of 87.62 cm when compared to Tricyclazole (74.91 cm). Nano-particles from C. lucknowense is being developed to be nano-elicitors for plant immunity.
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    Application of nano-particles derived from chaetomium elatum che01 to control pyricularia oryzae causing rice blast
    (2018-11-01)
    Song, J. J.
    ;
    Kanokmedhakul, S.
    ;
    Kanokmedhalkul, K.
    ;
    Soytong, K.
    Pyricularia oryzae causing rice blast was isolated and proved for pathogenicity. Chaetomium elatum ChE01 was proved to be antagonized P. oryzae in bi-culture antagonistic test which averaged inhibition of 60.40 % within 15 days. Fungal metabolites from C. elatum ChE01 were extracted and tested to inhibit P. oryzae. Results showed that crude ethyl acetate expressed antifungal activity against P. oryzae which the effective dose <inf>50</inf> (ED <inf>50</inf> ) was 231 ppm., followed by crude methanol and crude hexane which the ED <inf>50</inf> were 460 and 2,122 ppm. respectively. It was shown that nano-CCE gave the highest inhibition P. oryzae which the ED <inf>50</inf> was 8.25 ppm, and followed by nano-CEM and nano-CEH which the ED <inf>50</inf> values were 11.21 and 65.52 ppm, respectively. Further research findings are investigated in pot and field experiments.