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Biosensors for Klebsiella pneumoniae with Molecularly Imprinted Polymer (MIP) Technique
(2022-06-01)
Pintavirooj, Chuchart
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Vongmanee, Naphatsawan
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Sukjee, Wannisa
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Sangma, Chak
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Visitsattapongse, Sarinporn
Nosocomial infection is one of the most important problems that occurs in hospitals, as it directly affects susceptible patients or patients with immune deficiency. Klebsiella pneumoniae (K. pneu-moniae) is the most common cause of nosocomial infections in hospitals. K. pneumoniae can cause various diseases such as pneumonia, urinary tract infections, septicemias, and soft tissue infections, and it has also become highly resistant to antibiotics. The principal routes for the transmission of K. pneumoniae are via the gastrointestinal tract and the hands of hospital personnel via healthcare workers, patients, hospital equipment, and interventional procedures. These bacteria can spread rapidly in the hospital environment and tend to cause nosocomial outbreaks. In this research, we developed a MIP-based electrochemical biosensor to detect K. pneumoniae. Quantitative detection was performed using an electrochemical technique to measure the changes in electrical signals in different concentrations of K. pneumoniae ranging from 10 to 10<sup>5</sup> CFU/mL. Our MIP-based K. pneumoniae sensor was found to achieve a high linear response, with an R<sup>2</sup> value of 0.9919. A sensitivity test was also performed on bacteria with a similar structure to that of K. pneumoniae. The sensitivity results show that the MIP-based K. pneumoniae biosensor with a gold electrode was the most sensitive, with a 7.51 (% relative current/log concentration) when compared with the MIP sensor applied with Pseudomonas aeruginosa and Enterococcus faecalis, where the sensitivity was 2.634 and 2.226, respectively. Our sensor was also able to achieve a limit of detection (LOD) of 0.012 CFU/mL and limit of quantitation (LOQ) of 1.61 CFU/mL.
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Detection of Creatinine Using Molecularly Imprinted Polymers (MIP) Technique
(2022-01-01)
Pitayataratorn, Teerachote
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Sukjee, Wannisa
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Sangma, Chak
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Visitsattapongse, Sarinporn
An electrochemical potentiometric biosensor based on the molecularly imprinted polymer (MIP) technique has been fabricated for creatinine detection. The polymer consists of azobisisobutyronitrile (AIBN) as an initiator together with N, N'(1,2-Dihydroxyethelene) bisacrylamide (DHEBA) as a cross-linker and graphene oxide (GO) was prepared along with several functional monomers combination to compare each combination's effectiveness in the detection of creatinine. An experiment was divided into imprint and non-imprint polymer for imprinting effectiveness evaluation. Creatinine anhydrous were used as template molecules for imprinting the polymer. The analyte was prepared in buffer solution (PBS) at a pH of 7.4 with a concentration range from 0.01 mg/dl to 100 mg/dl. N-hydroxy succinimide (NHS) and D-glucose were used for the specificity test. This study can conclude that polymers consisting of functional monomer methyl methacrylate (MMA) and acrylamide (AAM) with a 1:1 ratio show significant sensitivity to creatinine with the detection limit of 0.1 mg/dl along with remarkable selectivity to creatinine against other negative control compared to other conditions in this study and the sensor has a response linearly ranges from 0.01 to 100 mg/dl.
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IDE Gas Sensor Based Dengue Virus Co-imprinting for Detection of 2,4,6-Trinitrotoluene
(2021-03-10)
Puttasakul, Tasawan
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Sukjee, Wannisa
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Pintavirooj, Chuchart
;
Sangma, Chak
In this work we present a method for explosive vapor detection by molecularly imprinted polymer (MIP) gas sensor. The explosive compound under investigation, 2,4,6-trinitrotoluene (TNT), in combination with dengue virus (DENV) was imprinted on polymer nanocomposites surface. This technique can create more TNT binding sites on the polymer structure. Thus, increasing the efficiency of template recognition. The TNT sensor was fabricated by coating the TNT MIP-composites on the surface of interdigitated electrodes (IDE). TNT detection was carried out by observing the capacitance change after TNT vapor expose. The result show that this technique can be used to detect TNT.
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Detection of 2,4,6-Trinitrotoluene by MIP-composite Based Electrochemical Sensor
(2021-03-10)
Puttasakul, Tasawan
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Tancharoen, Chompoonuch
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Sukjee, Wannisa
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Pintavirooj, Chuchart
;
Sangma, Chak
In Thailand, there are terrorist groups active in some areas where casualties from their operations are civilians. Most of these casualties are from explosive device. The main component of the device is high explosive compound called 2,4,6-trinitrotoluene (TNT). Detection of TNT from objects and places in the suspected or area bombardment will provide useful evidence for the authority. In this work, we fabricate an electrochemical TNT sensor based on molecularly imprinted polymers (MIP) nanocomposites. The sensor aimed at TNT trace detection from solution of solid specimens. The sensor was able to detect TNT in solution with 3 log-scale linearity with 0.4 ppt lower limit of detection. The selectivity of the sensor toward TNT was considerably high compared with the other nitro compound.
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Hydrogel Based-Electrochemical Gas Sensor for Explosive Material Detection
(2019-10-01)
Puttasakul, Tasawan
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Pintavirooj, Chuchart
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Sangma, Chak
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Sukjee, Wannisa
In Thailand, most of the bomb devices produced by the terrorists use trinitrotoluene (TNT) as the main explosive material. Detecting TNT and preventing detonation could save many civilians. Due to its low vapor pressure, TNT requires an extremely high sensitivity method for detection. In this paper, we propose an application of the polyacrylamide gel as an electrochemical gas sensor for explosive material detection. Cyclic voltammetry signals were collected from samples, including TNT, pentaerythritol tetranitrate (PETN), and non-explosive vapors. It was found that a time-derivative calculation of the signal was unique to each chemical, rather than the signal itself, demonstrating utility for the detection of explosive material. This approach for making a gel-based sensor and the data analysis looks promising and requires further validation.
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An influenza A virus agglutination test using antibody-like polymers
(2017-10-13)
Sukjee, Wannisa
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Thitithanyanont, Arunee
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Wiboon-ut, Suwimon
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Lieberzeit, Peter A.
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Paul Gleeson, M.
Antibodies are commonly used in diagnostic routines to identify pathogens. The testing protocols are relatively simple, requiring a certain amount of a specific antibody to detect its corresponding pathogen. Antibody functionality can be mimicked by synthesizing molecularly imprinted polymers (MIPs), i.e. polymers that can selectively recognize a given template structure. Thus, MIPs are sometimes termed ‘plastic antibody (PA)’. In this study, we have synthesized new granular MIPs using influenza A virus templates by precipitation polymerization. The selective binding of influenza A to the MIP particles was assessed and subsequently contrasted with other viruses. The affinities of influenza A virus towards the MIP was estimated based on an agglutination test by measuring the amount of influenza subtypes absorbed onto the MIPs. The MIPs produced using the H1N1 template showed specific reactivity to H1N1 while those produced using H5N1 and H3N2 templates showed cross-reactivity.

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