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Oil-in-water nanoemulsion-based ethyl lauroyl arginate nanoparticles exhibit potent antibacterial and antibiofilm activity against oral pathogens
(2026-04-23)
Wongkaewkhiaw, Saharut
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Pairojana, Tanita
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Chansiri, Sirirat
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Tsao, Stephany
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Panmekiate, Soontra
Background. Dental biofilm is a key etiological factor in oral diseases such as dental caries and periodontal disease, and has also been linked to systemic health complications. In this study, ethyl lauroyl arginate nanoparticles (ELANPs) were formulated using an oil-in-water nanoemulsion system to investigate their antibiofilm capacity and cellular cytotoxicity in vitro. Methods. The morphology of ELANPs was examined using transmission electron microscopy (TEM). Particle size, polydispersity index (PDI), and zeta potential were evaluated to assess nanoparticle stability over time. Biofilm matrix reduction was analyzed using confocal laser scanning microscopy (CLSM), and cytotoxicity was assessed using the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay. Results. ELANPs displayed a spherical morphology with an average diameter of 84.3 ± 2.6 nm. The zeta potential was 46.7 ± 4.6 mV, and the PDI was 0.18 ± 0.01, indicating good colloidal uniformity and stable nanosuspensions. Slight changes in physicochemical properties were observed at days 14 and 30, and overall stability was maintained. ELANPs significantly reduced the biofilm matrix across all tested oral pathogens while maintaining low cytotoxicity toward normal human gingival fibroblasts. Conclusion. The findings indicate that ELANPs are physicochemically stable, capable of inhibiting oral biofilm formation, and exhibit minimal cytotoxicity, supporting their potential as a safe and effective oral healthcare agent.
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Nanoparticles Derived from Active Metabolites of Chaetomium cupreum CC3003 against Phytophthora Rot of Durian
(2022-01-01)
Tongon, Rujira
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Soytong, Kasem
Phytophthora rot of durian (Durio zibetinus L.) is a serious disease wherever the crop has been planted and the disease control customarily uses chemical fungicides reported to be resistant by pathogen. Alternative non-chemical control strategies are being investigated to produce safe food. The main objective of this research was to test the activity of metabolites from Chaetomium cupreum CC3003 in the form of crude materials and nanoparticles to control and induce immunity to Phytophthora palmivora causing rot of durian var. Monthong. The results showed that P. palmivora proved to be pathogenic to durian var. Monthong. C. cupreum CC3003 acted as an antagonist and P. palmivora was confirmed as the pathogen by morphological and molecular genetic identification. Effective doses (ED50) of CC-E, CC-H and CC-M crude metabolites for spore inhibition were 60, 97 and 140 mg.kg-1, respectively. The research findings found that the diameters of nano CC-E, nano CC-H and nano CC-M were 534, 499 and 537 nm, respectively. The nano CC-E, nano CC-H and nano CC-M demonstrated antifungal activity against P. palmivora with ED50 of 11, 13 and 16 mg.kg-1, respectively. The nanoparticles at low concentrations were more effective than crude metabolites at high concentrations. Nano-CC-E used to treat seedlings of durian resulted in the production of scopoletin which served as an immunity agent or elicitor against rot disease of durian. It is concluded that active metabolites derived from C. cupreum significantly inhibited P. palmivora and induced immunity through phytoalexin production
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Electrochemical mechanisms of activated carbon, α-MnO2 and composited activated carbon-α-MnO2 films in supercapacitor applications
(2021-12-30)
Tagsin, Patin
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Suksangrat, Pitphichaya
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Klangtakai, Pawinee
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Srepusharawoot, Pornjuk
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Ruttanapun, Chesta
Pure α-MnO<inf>2</inf> and activated carbon-MnO<inf>2</inf> (AC-MnO<inf>2</inf>) films coated on Ni foam by electrophoretic deposition were applied as a supercapacitor electrode. The specific capacitance of AC-MnO<inf>2</inf> films (155.03 F g−1) surpasses those of the pure AC (110.62 F g−1) and pure MnO<inf>2</inf> film in the 1 M NaOH electrolyte. EDX and XPS detect an increase in the Na content and the reduction of Mn4+ to Mn3+ on the discharged MnO<inf>2</inf> electrode (at 0.0 V), whereas a decrease in the Na content and the oxidation of Mn3+ to Mn4+ were obtained on the charged MnO<inf>2</inf> electrode (at 0.45 V). Computational simulation of the Na inserted α-MnO<inf>2</inf> structure displays the connection of Na to O atoms and the increasing electron density on Mn atoms. EDX of the charged AC-MnO<inf>2</inf> (at −1.0 V) film detects a rise in the Na and a fall in the O contents, but the discharged AC-MnO<inf>2</inf> film (at 0.0 V) shows a decrease in Na and increase in O contents. The AC-MnO<inf>2</inf> film could retain 82.29% of the initial specific capacity after 10,000 cycles. Four series-supercapacitor coin cell assembled from the AC-MnO<inf>2</inf> anode and MnO<inf>2</inf> cathode delivers a power density of 2.79 kW kg−1 and an energy density of 168.8 Wh kg−1.
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Natural product of nano-particles constructed from chaetomium spp. To control rice blast disease caused by magnaporthe oryzae
(2020-01-01)
Song, Jiao Jiao
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Soytong, Kasem
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Kanokmedhakul, Somdej
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Kanokmedhkul, Kwanjai
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Poeaim, Supattra
Nanoparticles containing active compounds derived from Chaetomium cochliodes (CTh05) was tested to control rice blast disease caused by Magnaporthe oryzae isolate PO1. The causal agent of rice blast on leaves of rice var. RD57 was isolated. M. oryzae and C. cochliodes were identified morphologically and confirmed by molecular phylogenetic. M. oryzae was demonstrated to be pathogenic, causing blast of rice var. RD57. Biculture tests demonstrated that C. cochliodes could suppress the growth of M. oryzae. The crude metabolites of C. cochliodes (CCoH, CCoE and CCoM) at the concentrations of 10-1000 ppm expressed antifungal activity against the M. oryzae, resulting in inhibited spore production in 12 days with effective dose (ED<inf>50</inf>) values of 85, 144 and 374 ppm, respectively. The nanoparticles sizes of nano-CCoH, nano-CCoE and nano-CCoM were ranged between 567-611, `422-566 and 415-472 nm, respectively. Nanoparticles derived from C. cochliodes (nano-CCoH, nano-CCoE and nano-CCoM) at concentrations of 3-15 ppm significantly inhibited M. oryzae in 12 days with ED<inf>50</inf> values of 9, 16 and 33 ppm, respectively. In vivo experiments revealed a reduction of 38% in blast disease after application of nanoparticles constructed from crude extract mixtures from C. cochliodes at 10 ppm for 30 days. Tricyclazole resulted in reduction of blast disease by 29%. Rice blast disease was decreased in 30 days after applying nano-CCoM at the concentration of 7 ppm (60% disease reduction), followed by nanoCCoE and nanoCCoH with disease reduction of 58 and 50% respectively, and tricyclazole resulted in a 56% reduction in rice blast.

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