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    Item type:Publication,
    Impact of botanical essential oils on pupation and survival of Musca domestica L.
    (2026-05-01)
    Moungthipmalai, T.
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    Sriboonjit, N.
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    Khuendee, P.
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    Phunglakchai, J.
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    Thongsaiklaing, M.
    Among the tested botanical extracts, Illicium verum (star anise) essential oil at 10% demonstrated the highest efficacy in disrupting normal pupal development. Only 65.00% of individuals successfully emerged as fully developed adults after a 10-day exposure period. A notable proportion exhibited transitional deformities, particularly malformed pupal-adult intermediates, indicating interference with metamorphic progression. In comparison, cypermethrin, a commonly applied synthetic insecticide at the same concentration, resulted in a lower mortality rate of 39.67% under identical experimental conditions. The superior performance of star anise oil highlighted its potential as a plant-derived alternative for insect control. These findings suggested that the incorporation of such natural products into integrated pest management strategies may reduce dependence on conventional synthetic chemicals.
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    Item type:Publication,
    Plant essential oils, trans-anethole and eugenol, for housefly knockdown and mortality
    (2025-03-01)
    Sittichok, S.
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    Sinthusiri, J.
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    Thongsaiklaing, T.
    The knockdown and mortality of some compounds from natural oils (trans-anethole, eugenol and a combination of trans-anethole and eugenol) against adult houseflies (Musca domestica) compared with cypermethrin was investigated. The knockdown and mortalities were highest at 100% of 1% trans-anethole with KT50= 31 min and LT50 = 31 min. The mortality index of trans-anethole was 82.1 times that of cypermethrin. In contrast, 1% eugenol had knockdown and mortality was 36% and 0.5% trans-anethole + 0.5% eugenol had knockdown and mortality of 68%, with mortality indexes of 1.6 and 3.2 times versus cypermethrin. The trans-anethole high toxicity and low residue made it a superior control for houseflies.
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    Item type:Publication,
    Adulticidal activity of star anise, turmeric, cloves and combinations against houseflies
    (2025-01-01)
    Sinthusiri, J.
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    Puwanard, C.
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    Thongsaiklaing, T.
    The small droplet size could increase the absorbed ingredients and high stability. The star anise nanoemulsion was determined optimum efficacy, with a 1% concentration resulting in the highest knockdown and mortality rate of 100%. Its KT<inf>50</inf> and mortality effective values were 28 min and 36 times, respectively. Furthermore, the adult stage of houseflies is found to be susceptible to star anise nanoemulsion. In contrast, cypermethrin was less toxic to it, with a high KT<inf>50</inf> and resistance to adult houseflies. Consequently, the star anise nanoemulsion could be developed into an efficient and safe environment for controlling housefly populations.
      1
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    Item type:Publication,
    The effectiveness of star anise nanoemulsion and chemical insecticide for controlling of beet armyworm
    (2024-05-01) ;
    Pumnuan, J.
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    Thongsaiklaing, T.
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    Thipmanee, K.
    The effectiveness of star anise (Illicium verum) nanoemulsion and deltamethrin (chemical insecticide) was tested with beet armyworm (Spodoptera exigua) to evaluate their mortality, antifeedant, and growth inhibition effects at proper concentrations. As a result, at the obtained highest effectiveness, star anise nanoemulsion required 0.35% concentration for the maximum effectiveness with 100% mortality, antifeedant and growth inhibition effects against beet armyworm, when compared with the control (Tween20 and water), while deltamethrin exhibited a lower rate. Therefore, star anise nanoemulsion seem to be utilized as a highly effective and environmentally friendly insecticide to control beet armyworm.
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    Item type:Publication,
    Insecticidal efficiency of plant essential oil nanoemulsion formulas against Spodoptera exigua
    (2024-01-01) ;
    Pumnuan, J.
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    Thongsaiklaing, T.
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    Thipmanee, K.
    The findings revealed that the nEO formula of I. verum and C. longa demonstrated optimal effectiveness, with a concentration of 0.35% resulting in the highest mortality rate of 43.3%, 100% antifeedant effect and 85.0% growth inhibition activities in pupae. Additionally, a concentration of 0.25% led to 100% growth inhibition activities in adults. Consequently, the nEO formulas of I. verum and C. longa promise for the future development of botanical insecticides targeting the control of S. exigua.
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    Insecticidal effect of plant essential oil nanoemulsions on controlling Spodoptera exigua
    (2023-03-01) ;
    Pumnaun, J.
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    Thipmanee, K.
    The results showed that Curcuma longa and Illicium verum nEOs performed extremely high mortality and antifeedant activity, as well as affected on growth development of Spodoptera exigua. Essential oil nanoemulsions at 0.35 % concentrations caused 100% mortality, 100% antifeedant activity, and 100% growth inhibition. Curcuma longa and Illicium verum nEOs revealed to be used as botanical insecticide for controlling Spodoptera exigua.
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    Evaluating the acaricidal effect of essential oil nanoemulsion against the cutworm, Spodoptera litura
    (2022-09-01) ;
    Pumnaun, J.
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    Thipmanee, K.
    The cutworm, Spodoptera litura is an important pest of cruciferous vegetables and it causes economic impact worldwide. The efficiency of turmeric and star anise essential oil nanoemulsions (nEOs) against the cutworm, including mortality, antifeedant, and growth inhibition effects was investigated. Essential oil nanoemulsions (nEOs) were prepared by mixing the essential oils with surfactants and co-surfactants. The 2<sup>nd</sup>, stage instar larvae of cutworms were tested by leaf dipping method and were observed after 24 hours. The results showed that the two nEOs, which were turmeric and star anise, performing the mortality, and growth development effect on Spodoptera litura as well as having antifeedant property. Complete mortality rate was shown at 0.35 % concentration of turmeric and star anise nEOs, when 100% antifeedant effect was found at 0.20% concentrations of nEOs, and 100% growth inhibition was observed at 0.30% concentrations of nEOs. It could be concluded that the nEOs from star anise had antifeedant and insecticide properties.
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    Item type:Publication,
    Quality change on holy basil after fumigation with insecticidal essential oil formula from clove and cinnamon
    (2018-04-04)
    Pumnuan, J.
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    Insung, A.
    The essential oil formula from clove (Syzygium aromaticum) and cinnamon (Cinnamomum bejolghota) at the ratio of 1:3 (Cl1Ci3) has been recently considered as an effective alternative fumigant against thrips (Thrips palmi) and aphid (Aphis gossypii), which are the major pests attacking exported holy basil (Ocimum sanctum) in Thailand. This study aimed to investigate the effects of the C1Ci3 formula used as fumigant on physical quality of holy basil compared to methyl bromide application. The percentage of insect mortality and change in L*, a* and b* values, as well as weight loss of holy basil, were determined. All fumigations in this study were conducted using the formula Cl1Ci3 at the concentrations of 0.0, 2.0 and 3.0 mL m<sup>-3</sup> for 2 h with 15-min air circulation in 1 m<sup>3</sup> fumigation chamber and compared to methyl bromide at 20 g m<sup>-3</sup> (recommended concentration). The observations of insect mortality were conducted at 24 h after treatment. The holy basil samples were evaluated prior to the treatments, immediately after the treatments, and after 3 and 6 days at 25±1°C. The methyl bromide fumigation showed 100% mortality of all insects and its efficacy was significantly higher than that of the Cl1Ci3 formula (73.9-96.0%). Leaf burning was observed immediately after methyl bromide fumigations. On the other hand, no significant physiological changes were observed on plants fumigated with the Cl1Ci3 formula, as compared to the control.
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