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    studies on the Rapid and simple DNA extraction Method, Antibacterial Activity and enzyme Activity involved in Plant Biomass Conversion by Cookeina sulcipes and C. tricholoma (Cup Fungi)
    (2022-12-01)
    Tangthirasunun, Narumon
    ;
    Poeaim, Supattra
    Cookeina sulcipes and C. tricholoma are a cup fungi (Ascomycota) collected in saraburi, thailand. the fungi have been isolated, cultured and confirmed as respective species. For morphology, both Cookeina sp. are white mycelium and the growth rate on potato dextrose agar (PDA) result present C. sulcipes is faster than C. tricholoma. the molecular characterization from a rapid and simple DNA extraction method that is modified based on thermolysis method, the DNA extraction is finish in thirty minutes and efficiency to continuous with polymerase chain reaction (PCR) amplification to fungi species level identification. the DNA sequence from internal transcribed spacer (its) gene regions by universal primer pairs its5/its4 is effective to confirm Cookeina species level that C. sulcipes has 616 bp and C. tricholoma has 570 bp. including, DNA sequence of large subunit (lsU) gene regions by universal primer pairs lROR/lR5 is generate that C. sulcipes has 912 bp and C. tricholoma has 906 bp. the cultures are screened for antibacterial activity by agar plug diffusion method and found that both isolates have been no activity against test strains (Bacillus subtilis, Escherichia coli, Kocuria rhizophila (Micrococcus luteus), Pseudomonas aeruginosa, Staphylococcus aureus and S. epidermidis). in a preliminary screening test of enzymes involved in plant biomass breakdown by agar plate method, both Cookeina sp. show cellulolytic and hemicellulolytic enzymatic activity, and manganese peroxidase (MnP) productivity. in contrast, only C. sulcipes had additional laccase activity. Neither isolate generate pectinolytic and lignin peroxidase (liP) activities. thus, Cookeina spp. proved the potentiality to break down lignocelluloses.
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    Agaricus flocculosipes sp. nov., a new potentially cultivatable species from the palaeotropics
    (2012-01-01)
    Zhao, Rui Lin
    ;
    Hyde, Kevin D.
    ;
    Desjardin, Dennis E.
    ;
    Raspé, Olivier
    ;
    Soytong, Kasem
    Agaricus flocculosipes is described as new from northern Thailand and Mayotte based on morphology and ITS sequence analysis. The new species is compared with similar taxa, and a comprehensive description, line drawings, and colored photographs are provided. Agaricus flocculosipes is distinguished among the species in section Arvenses by its relatively large sporocarps (110-180 mm in diameter), erect floccose squamules on the surface of the stipe, and relatively small basidiospores (less than 6.5 μm long). Phylogenetic analyses based on ITS sequence data using maximum likelihood, parsimony, and Bayesian analyses all support A. flocculosipes as being a distinct species within section Arvenses. The new taxon is potentially cultivatable as it is presumably heterothallic, exhibits extensive genetic diversity, and occurs in section Arvenses, a lineage that contains other edible cultivatable taxa such as Agaricus arvensis and Agaricus subrufescens. © 2012 The Mycological Society of Japan and Springer.
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    A new species of bird's nest fungi: Characterisation of Cyathus subglobisporus sp. nov. based on morphological and molecular data
    (2008-12-01)
    Zhao, R. L.
    ;
    Desjardin, D. E.
    ;
    Soytong, K.
    ;
    Hyde, K. D.
    Recent collections of bird's nest fungi (i.e. Crucibulum, Cyathus, Mycocalia, Nidula, and Nidularia species) in northern Thailand resulted in the discovery of a new species of Cyathus, herein described as C. subglobisporus. This species is distinct by a combination of ivory-coloured fruiting bodies covered with shaggy hairs, plications on the inner surface of the peridium and subglobose basidiospores. Phylogenetic analyses based on ITS and LSU ribosomal DNA sequences using neighbour-joining, maximum likelihood and weighted maximum parsimony support Cyathus subglobisporus as a distinct species and sister to a clade containing C. annulatus, C. renweii and C. stercoreus in the Striatum group. © 2008 Nationaal Herbarium Nederland Centraalbureau voor Schimmelcultures.
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