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    Mulberroside A Accumulation in Mulberry Plantlets from In Vitro Culture and Hydroponic System
    (2023-12-29)
    Damrongmongcolkul, Natvara
    ;
    Phunpruch, Saranya
    ;
    Lohasupthawee, Pana
    In this study, nodal explants cultured in MS medium supplemented with BAP 4mg/l and NAA 0.5 mg/l induced 3.33 shoots per explant within 4 weeks. The elongated multiple shoots were transferred to rooting media. The best root development was found on MS medium without PGRs within 10 days. The acclimatized plants were transferred to a deep-water hydroponic system with different concentrations of MS nutrient solution without sucrose addition. The results showed that mulberry plants cultivated in 1/8 MS nutrient solution gave the highest growth index. For mulberroside A determination, the results showed that mulberroside A accumulation in roots were higher than in shoots of mulberry plants both for in vitro culture and in the hydroponic system. In the case of in vitro culture, mulberroside A accumulation in roots was 20.61 mg/g DW, and in shoots was 11.98 mg/g DW. In hydroponic system, mulberroside A accumulation in roots was 17.58 mg/g DW, and in shoots was 2.34 mg/g DW. We also found that the higher concentration of MS nutrient solution, the more mulberroside A accumulation in roots of mulberry plants cultivated in the hydroponic system.
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    A new method to increase plant quantity through tissue culture on Vitex negundo L
    (2022-09-01)
    Poeaim, A.
    ;
    Prasertkittikul, N.
    ;
    Wangsor, N.
    ;
    Boonmee, W.
    ;
    Laipasu, P.
    A new method to increase plant quantity through tissue culture on Vitex negundo L. was discovered. The influences of PGRs on shoot regeneration cultured on MS medium with 0.5, 1, 2, or 3 mg/L benzylaminopurine (BAP), meta-Topolin (mT) and Thidiazuron (TDZ) were resulted to be the greatest percentage of shoot development was 100% at 1 mg/L BAP in MS medium. The greatest average number of shoot multiplication was 4.40 shoots at 0.5 mg/L BAP in MS medium after 8 weeks of culture. For roots, the result of MS media fortified with 0.5, 1, 2, and 3 mg/L of indole 3 butyric acid (IBA) and naphthalene acetic acid (NAA) discovered that the excellent percentage of root production was 90% at 3 mg/L IBA. The highest average number of root production was 108.20 roots on 2 mg/L NAA after 8 weeks of culture. The plantlets were eventually transferred into the plastic pots. In this research work, the nodes were demonstrated to be an outstanding regenerating explant resource for Vitex negundo L. An effective regeneration strategy for plantlets derived from the nodes was demonstrated.