Kramchote, Somsak
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Kramchote, Somsak
Alternative Name
Kramchote, S.
Main Affiliation
Email
somsak.kr@kmitl.ac.th
15 results
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Item type:Publication, Field Performance and ecological safety of botanical nanoemulsions against sweet potato weevil in Tropical smallholder systems(2026-06-01) ;Pumnuan, Jarongsak ;Lakyat, Anuwat ;Name, Janejira ;Deemak, JantraPhutphat, SudjaiThis study investigated the effectiveness of two nanoemulsion-based plant hexane extract formulations, S4L0 and S3L1, for managing sweet potato weevils (SPW) under field conditions. The formulations, derived from star anise and long pepper hexane extracts, were tested alongside a synthetic insecticide (imidacloprid) and an untreated control using a randomized complete block design (RCBD) on farmer plots in Sa Kaeo Province, Thailand. Key determination parameters included SPW infestation levels, crop yield, pesticide residue levels, diversity of non-target soil arthropods, and soil physicochemical properties. Field applications of S4L0 and S3L1 reduced SPW infestations to 3.6 ± 4.3 and 11.1 ± 5.0 insects per plant, respectively, significantly lower than in untreated plots (33.9 ± 11.8 insects/plant). No detectable imidacloprid residues were found in tubers treated with S4L0 or S3L1, whereas imidacloprid-treated tubers retained residues of 0.335 and 0.144 ppm at 5 and 7 days after application. Sweet potato yields ranged from 310 to 440 g/plant, with no significant differences among treatments. The untreated plots supported greater diversity and abundance of non-target soil arthropods, while the imidacloprid-treated plots showed the lowest diversity. Soil properties, including bulk density, pH, electrical conductivity, and organic matter content, remained stable across treatments. Overall, S4L0 and S3L1 demonstrated effective pest suppression and minimal environmental impact, highlighting their potential as sustainable alternatives to synthetic insecticides for integrated pest management in sweet potato production. - Some of the metrics are blocked by yourconsent settings
Item type:Publication, Optimization of Feed Formulation, Feeding Rate, and Plant-Based Supplements for Efficient Rearing of the Superworm Zophobas morio (Fabricius) Under Tropical Conditions(2026-02-01) ;Pumnuan, Jarongsak ;Prachom, NoratatInsects are increasingly recognized as sustainable protein sources due to their high feed conversion efficiency and low environmental impact. Among them, the superworm, Zophobas morio (Fabricius) (Coleoptera: Tenebrionidae), has strong potential for large-scale production; however, optimized feeding strategies under tropical conditions remain limited. This study aimed (1) to determine the optimal feed formulations and feeding rate using wheat bran supplemented with the KMITL Protein Innovation source (a protein feed ingredient developed by the School of Agricultural Technology, King Mongkut’s Institute of Technology Ladkrabang, KMITL), and (2) evaluate the influence of plant-based supplementary foods on larval performance. In Phase I, larvae were reared on 13 formulations with three protein levels (CP00, CP21, and CP24) and five feeding rates (A–E). Diets CP21–21 and CP24–21 (21 and 24% CP; wheat bran/protein = 2:1) resulted in the highest survival (83.4–84.1%) and the lowest feed conversion ratios (FCR = 2.29–2.34). Moderate feeding rates (C–D; 925–1110 g feed per tray for 50 days) produced the greatest larval weights (700–760 mg), whereas ad libitum feeding provided no additional benefit. In Phase II, larvae reared on CP21–21 with a restricted rate of 1100 g per tray and supplemented with ten plant-derived foods achieved comparable final weights (716–760 mg), but survival varied significantly among treatments. Mulberry leaf yielded the highest survival (95.3%), followed by banana, watermelon rind, winter melon, and jicama (>90%). Pumpkin and jicama accelerated pupation and adult emergence, showing a female-biased sex ratio among emerged adults (59.2–65.5%), suggesting enhanced developmental rates. These results establish a practical framework for cost-effective and sustainable Z. morio production under tropical conditions, contributing to circular bioeconomy strategies and supporting insect-protein innovation. - Some of the metrics are blocked by yourconsent settings
Item type:Publication, Combining ability analysis in anthracnose-resistant introgression inbreds and developing improved resistant lines of pepper (Capsicum annuum L.)(2025-01-01) ;Saengarun, Patcharapan; ;Inprasert, Hathaipat ;Kumsee, NattikaTsai, Wen ShiChili peppers (C. annuum L.) are a commercial spice crop in the tropics. In Thailand, chili pepper is a cash crop for farmers and has cultural significance in Thai cuisine. Anthracnose disease caused by Colletotrichum spp. is responsible for a significant reduction in the marketable fruit yield of chili peppers. In this study, we tested the combining abilities of anthracnose-resistant chili pepper lines possessing resistance introgression genome segments from C. chinense (PBC932) and C. baccatum (PBC80). Six parental inbreds developed from two resistant donors PBC80 and PBC932 were crossed in a haft-diallel fashion to develop 15 cross combinations during 2018. In 2019, seeds of 21 genotypes comprising six parents and 15 F<inf>1</inf> s were planted in Randomized Complete Block Design (RCBD) and data on plant characteristics, fruit yield and yield components were recorded. Detached fruits were screened against anthracnose causing aggressive species (C. acutatum). ANT4 showed the lowest disease lesion size and lowest GCA value at green and red fruit stages, containing resistance from PBC80. Additionally, the general combining ability (GCA) and specific combining ability (SCA) were significantly different at the green fruit stage, with the SCA component greater than GCA. Based on GCA and SCA estimates, ANT4 was selected and crossed to three highly susceptible cultivars (Yodsonkeam 80, Num, and Jindanil 80). The crosses were advanced and 10 F<inf>5</inf> lines were developed through marker-assisted selection (MAS) for resistance genes. The MAS was more useful for selecting resistance at the green fruit stage. We also found that MAS was more effective in F<inf>2</inf> progenies compared to F<inf>5</inf> progenies. Finally, we selected 10 new pure lines and evaluated them at the farmer's field. AF1, AF6, and AF7 were highly resistant to anthracnose, a more desirable good fruit type and high yield. - Some of the metrics are blocked by yourconsent settings
Item type:Publication, Improving Pepper Inbreds for Resistance to Pepper Yellow Leaf Curl Thailand Virus (PepYLCTHV) through Challenged Inoculations(2024-09-01) ;Kumsee, Nattika; ; ;Tsai, Wen ShiTechawongstien, SuchilaChili peppers (Capsicum annuum L.) are an economically important crop worldwide. Pepper yellow leaf curl Thailand virus (PepYLCTHV), a Begomovirus causing yellow leaf mosaic disease of chili pepper, has been reported to incur 95% economic loss under epiphytotic conditions. Thirty-one chili genotypes were screened for resistance to PepYLCTHV disease through inoculation using 10–15 viruliferous whiteflies per plant. We purified two resistant lines (PEP6 and PEP12) through four generations of selfing and selection. At 28 days after inoculations, two chili genotypes (PEP6 and PEP12) had low disease severity and percentage of disease incidence (DI) compared to four susceptible checks, viz., Yodsonkeam80, Homsupan, Huareau12, and Pong Charian, which had a disease severity score of 5 with 100% DI. Thirty initial plants of PEP6 showed an average disease severity of 3.64 with 69.33% DI, and PEP12 showed an average disease severity of 3.83 with 77.67% DI. From these populations, we selected nine highly resistant plant of PEP6 and seven plants of PEP12 having a disease severity of 0 through pure-line selection for four selfing generations. The ratio of resistance (R) to susceptibility (S) consequently decreased. In PEP6, the ratio decreased from 1R:2S to 1R:1S, while in PEP12 the ratio decreased from 1R:3S to 1R:1S. These lines have potential for release as resistant lines for improving chili pepper resistance to PepYLCTHV and for developing makers associated with the resistant trait. - Some of the metrics are blocked by yourconsent settings
Item type:Publication, Using a Computer Vision System for Monitoring the Exterior Characteristics of Damaged Apples(2025-10-01) ;Al-Riyami, Zamzam ;Al-Dairi, Mai ;Pathare, Pankaj B.Mechanical damage like bruises produced during postharvest handling can lower market value, affect nutritional value, and pose food safety risks. The study evaluated bruises on apples using image processing. This research focuses on using computer vision for apple fruit damage detection. The fruits were subjected to three levels of impact using three ball weights (66, 98, and 110 g) dropped from 50 cm height and stored at 22 °C. The overall impact energies generated were 0.323 J (low), 0.480 J (medium), and 0.539 J (high). The bruise area and susceptibility of the damage, surface area of the fruit, and color were measured manually (colorimeter) and by image processing. The study found that the bruise area was significantly affected by impact force, where 110 g (0.539 J) damaged apples showed a bruise area of 4.24 cm<sup>2</sup> after 21 days of storage at 22 °C. The images showed a significant change in the RGB values (Red, Green, Blue) over 21 days of storage when impacted at 0.539 J. The study showed that the greater the impact energy effect, the higher the weight loss under constant conditions of storage. After 21 days of storage, the 110 g mechanically damaged apples recorded the highest percentage of weight loss (6.362%). The study found a significant decrease in the surface area of 110 g bruised apples, with a smaller decrease in surface area for 66 g bruised fruit. The use of computer vision to detect bruise damage and other quality attributes of Granny Smith apples can be highly recommended to detect their losses. - Some of the metrics are blocked by yourconsent settings
Item type:Publication, In Vitro Termiticidal Activity of Medicinal Plant Essential Oils Against Microcerotermes crassus(2025-12-01) ;Chantarapitak, Chaiamon ;Pumnuan, Jarongsak ;Chanpitak, ChaiwatTermites are destructive structural pests causing significant economic losses worldwide. Although synthetic termiticides are effective, their extensive use raises environmental and health concerns, highlighting the need for safer, sustainable alternatives. This study evaluated the contact toxicity and repellency in a closed system of essential oils (EOs) from clove (Syzygium aromaticum), cinnamon (Cinnamomum zeylanicum), and star anise (Illicium verum) against Microcerotermes crassus workers. Chemical constituents (eugenol and anethole) were characterized by GC–MS/MS, and their activities were compared with those commercial termiticides (fipronil and cypermethrin). Clove and cinnamon oils exhibited strong toxicity, with LC<inf>50</inf> values decreasing from 208.6 to 70.6 µL/L and from 362.6 to 130.5 µL/L, respectively, over 24 h. Both achieved LT<inf>50</inf> values below 10 h at 250 µL/L and over 80% repellency at 100–500 µL/L within 3–12 h. Eugenol, the major constituent, showed comparable activity. In contrast, star anise oil, dominated by anethole, displayed weaker repellency but measurable termiticidal effects with a slower onset. These findings indicate that eugenol-rich oils may provide rapid-contact activity. In contrast, anethole-rich oils may offer slower but measurable termiticidal effects, suggesting distinct potential roles in future formulation development. The findings provide a foundation for developing nanoemulsion-based botanical termiticides with improved stability and field performance. - Some of the metrics are blocked by yourconsent settings
Item type:Publication, Long-term insect surveillance and facility-level risk assessment to support integrated pest management in a fruit juice manufacturing facility(2026-06-01) ;Chantarapitak, Chaiamon ;Pumnuan, Jarongsak ;Chanpitak, ChaiwatLong-term insect surveillance is a critical component of industrial pest management in food manufacturing facilities. This study assessed the effectiveness of an Integrated Pest Management (IPM) program implemented in a fruit juice factory in Pathum Thani, Thailand, from January 2023 to June 2025. Weekly monitoring using 28 insect light traps and sticky boards was conducted across the facility. Capture data were aggregated on a monthly basis. To reduce the confounding influence of seasonal variation, temporal trends were assessed using same-period half-year comparisons. Diptera, particularly fruit flies, was predominant (85.7%), followed by Hemiptera (including former Homoptera) (7.9%), Hymenoptera (1.7%), and Coleoptera (1.3%). Other insects occurred at very low frequencies, collectively accounting for < 1% of the total captures. Temporal analysis using same-period half-year comparisons showed reductions of −19.1%, −28.0%, and −12.6%, followed by stabilization in 2025, with a distinct peak in November 2023 suppressed by corrective measures such as fogging, light management, and door control. The response followed a stepwise IPM framework with intensified actions only when required. Spatial analysis showed higher captures in Building 2 and the staff canteen than in Building 1. Risk classification showed most traps remained within acceptable thresholds, while persistent Level 3 risks were limited to Traps 10, 18, and 19 in Building 2. These results indicate a progressive reduction in insect captures across successive monitoring intervals under the implemented IPM program, followed by stabilization at comparatively low levels. The study provides a long-term dataset and a practical framework for facility-level insect risk assessment, supporting evidence-based pest management in industrial settings. - Some of the metrics are blocked by yourconsent settings
Item type:Publication, Nanoemulsion-based plant essential oil formulations: in vitro evaluation of pesticidal activity against ectoparasites in poultry(2024-01-01) ;Lakyat, Anuwat ;Pumnuan, Jarongsak ;Doungnapa, Thanaporn ;Phutphat, SudjaiEctoparasite infestations significantly impact the health and productivity of poultry. Chemical applications, although common for pest control, lead to pesticide residues and parasite resistance in poultry. Nanoemulsion-based plant essential oil formulations (NEOFs) provide a promising alternative for controlling poultry ectoparasites. This study aimed to assess the efficacy of NEOFs from clove, cinnamon, and turmeric essential oils (EOs) against ectoparasites, Menopon gallinae and Megninia ginglymura, under laboratory conditions. The toxicity and repellent properties of the NEOFs were examined, with the major chemical compounds of the EOs analyzed using chromatography mass spectrometer. Results identified eugenol as the dominant component in clove and cinnamon EOs (84.60 and 75.19%, respectively), while turmerone (68.46%) was the major compound in turmeric EO. NEOFs with clove:cinnamon:turmeric ratios of 4:0:0, 2:2:0, and 2:0:2 had particle size of 20.76 nm, 20.66 nm, and 89.56 nm, respectively, while those based on eugenol and turmerone standards had sizes <21.0 nm. In addition, NEOFs at 0.3% concentration with ratios of 4:0:0 and 2:2:0 achieved full control of both ectoparasites. These formulas demonstrated exceptional potency in exterminating ectoparasites, with LC<inf>50</inf> and LC<inf>90</inf> at <0.160 and <0.250%, respectively, 6 h after treatments. Furthermore, both NEOFs showed higher repellence responses in M. gallinae compared to M. ginglymura. The toxicities of these NEOFs were comparably effective against both parasites, showing no significant difference compared with chemical insecticide treatment. Therefore, further research will explore the practicality of using clove and cinnamon-derived NEOFs under farm conditions. - Some of the metrics are blocked by yourconsent settings
Item type:Publication, Effect of Trichoderma harzianum against Fusarium oxysporum in resistant and susceptible tomato cultivars(2025-01-01); ;Mueangkhong, Manthana ;Kawicha, Praphat ;Thanyasiriwat, ThanwanitTechawongstien, SuchilaFusarium wilt disease presents a substantial challenge to tomato production, especially in an open field environment. The peroxidase (POD) activity and total phenolic compounds (TPCs) play a crucial role in measuring the antioxidant capacity of plants. Understanding the variations in the POD and TPC levels during disease-induced stress becomes important for effectively managing Fusarium wilt and enhancing tomato production. This study investigates the impacts of Trichoderma harzianum inoculation through the root drip method on five tomato cultivars. It compares these cultivars to their non-treated counterparts when they are subjected to infection by Fusarium oxysporum f. sp. lycopersici (Fol). The results showed that the level of resistance to Fol is based on the specific tomato cultivar. Notably, 'MT26' exhibited the lowest disease severity index (DSI), indicating a strong response, whereas 'CLN3682F' showed notable susceptibility. Regarding the POD and TPC activity, its exhibition differed in compatibility with the response of each tomato cultivar to Fusarium wilt disease. The resistant cultivars increased the POD activity after the Trichoderma induction before the Fol inoculation, and this activity was further boosted when exposed to disease conditions. Consequently, enhancing the POD and TPC levels during the initial stages could potentially serve as a systemic defence mechanism of tomatoes against the Fusarium wilt disease. - Some of the metrics are blocked by yourconsent settings
Item type:Publication, Delaying quality changes in ‘Namdokmai Sithong’ mango fruit during low-temperature storage using trisodium phosphate(2024-06-01) ;Ganjana, R.; This study explored the efficacy of trisodium phosphate (TSP) in maintaining the postharvest quality of ‘Namdokmai Sithong’ mangoes under low-temperature storage conditions. Employing a completely randomized design, mangoes were treated with TSP at 0 (control), 0.25, 0.50, and 0.75 g L<sup>-1</sup> and stored at 6±2°C with 85±5% RH for 25 days. Quality assessments, conducted every five days, focused on firmness, peel color, total soluble solids (TSS), hydrogen peroxide (H2O2), titratable acidity (TA), and phenolic content. Results showed that while TSP did not influence firmness, it significantly delayed peel color changes and affected TSS, H2O2, TA, and phenolic levels. The 0.50 and 0.75 g L<sup>-1</sup> concentrations were most effective, with no marked difference between them, suggesting a recommendation of 0.50 g L<sup>-1</sup> TSP for optimal cost-efficiency in preserving mango quality.
