Phunpruch, Saranya

Preferred name

Phunpruch, Saranya

Alternative Name

Phunpruch, S.

Main Affiliation

School of Science

saranya.ph@kmitl.ac.th

Now showing 1 - 6 of 6

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Enhanced Hydrogen Production by the Halotolerant Cyanobacterium Aphanothece halophytica Through Bacterial Co-Cultivation
(2026-05-01)
Somsin, Chutikarn
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Chinchusak, Nattanon
;
Incharoensakdi, Aran
;
Phunpruch, Saranya
Hydrogen (H<inf>2</inf>) is a promising clean energy carrier with the potential to partially replace fossil fuels. Biological H<inf>2</inf> production using microorganisms offers an environmentally friendly alternative. The halotolerant cyanobacterium Aphanothece halophytica can produce H<inf>2</inf> under nitrogen-deprived and dark anaerobic conditions. In this study, a co-culture strategy was investigated to enhance H<inf>2</inf> production. Five bacterial strains were screened for their ability to improve H<inf>2</inf> production when co-cultivated with A. halophytica. Among them, Staphylococcus aureus significantly enhanced H<inf>2</inf> production, achieving a maximum rate of 11.11 ± 0.18 µmol H<inf>2</inf> g−1 dry weight h−1. Optimization of the bacterial partner revealed that S. aureus cells harvested at 12 h in the mid-logarithmic phase with an OD<inf>600</inf> of 4.0 were the most effective. An inoculum ratio of A. halophytica to S. aureus of 4:1 further enhanced H<inf>2</inf> production, increased bidirectional hydrogenase activity, and reduced O<inf>2</inf> accumulation. Under optimal conditions (0.945 mmol C-atom L−1 glucose, 0.25 M NaCl, pH 7.4, and 35 °C), the maximum H<inf>2</inf> production rate reached 132.49 ± 4.45 µmol H<inf>2</inf> g−1 dry weight h−1, approximately 5.5-fold higher than that under normal conditions. The co-culture achieved a cumulative H<inf>2</inf> yield of 3248.51 ± 88.11 µmol H<inf>2</inf> g−1 dry weight after 48 h.
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Modified natural seawater as growth medium for the halotolerant cyanobacterium Aphanothece halophytica to increase lipid content for biodiesel production
(2025-02-01)
Thongtha, Sitthichai
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Aryusuk, Kornkanok
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Kittiwongwattana, Chokchai
;
Incharoensakdi, Aran
;
Phunpruch, Saranya
Biodiesel derived from cyanobacterial oils becomes attractive as an efficient renewable energy. The present study aims to optimize growth and lipid production of the halotolerant unicellular cyanobacterium Aphanothece halophytica cultivated in natural seawater. In this study A. halophytica was able to grow in natural seawater when supplemented with low concentration of NaNO<inf>3</inf>, whereas no growth occurred without supplementation. The specific growth rate of 0.230 day-1 and cell concentration of 25.17 x 106 cells mL-1 were achieved in A. halophytica cultivated in natural seawater supplemented with 17.6 mM NaNO<inf>3</inf> and Turk Island salt solution (suitable natural seawater; SNSW) for 14 days. This growth rate was comparable to that of cells grown in normal BG11 plus Turk Island salt solution. The lipid content and fatty acid profiles of A. halophytica varied with changes in NaCl concentrations. The highest lipid content of 50.47 % and lipid productivity of 48.33 mg L-1 day-1 were obtained in cultures supplemented with 1.89 mmol C-atom L-1 glucose and 0.75 M NaCl. The optimal medium pH and cultivation temperature for lipid production was 7.5 and 25-35 °C, respectively. When cultivating A. halophytica in optimized SNSW with various NaCl concentrations, the highest contents of linoleic and linolenic acids, and the lowest contents of palmitic, stearic, and oleic acids were observed with 0.75 M NaCl. In contrast, cultures grown in optimized SNSW with 0.5 M NaCl showed fatty acid methyl ester profiles rich in monounsaturated fatty acids, which are favorable for high-quality biodiesel production.
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Simazine Enhances Dark Fermentative H2 Production by Unicellular Halotolerant Cyanobacterium Aphanothece halophytica
(2022-07-15)
Pansook, Sunisa
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Incharoensakdi, Aran
;
Phunpruch, Saranya
The halotolerant cyanobacterium Aphanothece halophytica is a potential H<inf>2</inf> producer that induces H<inf>2</inf> evolution under nitrogen deprivation. H<inf>2</inf> is mainly produced via the catabolism of stored glycogen under dark anaerobic condition. H<inf>2</inf> evolution is catalyzed by O<inf>2</inf>-sensitive bidirectional hydrogenase. The aim of this study was to improve H<inf>2</inf> production by A. halophytica using various kinds of inhibitors. Among all types of inhibitors, simazine efficiently promoted the highest H<inf>2</inf> production under dark conditions. High simazine concentration and long-term incubation resulted in a decrease in cell and chlorophyll concentrations. The optimal simazine concentration for H<inf>2</inf> production by A. halophytica was 25 µM. Simazine inhibited photosynthetic O<inf>2</inf> evolution but promoted dark respiration, resulting in a decrease in O<inf>2</inf> level. Hence, the bidirectional hydrogenase activity and H<inf>2</inf> production was increased. A. halophytica showed the highest H<inf>2</inf> production rate at 58.88 ± 0.22 µmol H<inf>2</inf> g−1 dry weight h−1 and H<inf>2</inf> accumulation at 356.21 ± 6.04 μmol H<inf>2</inf> g−1 dry weight after treatment with 25 µM simazine under dark anaerobic condition for 2 and 24 h, respectively. This study demonstrates the potential of simazine for the enhancement of dark fermentative H<inf>2</inf> production by A. halophytica.
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Dark fermentative hydrogen production and transcriptional analysis of genes involved in the unicellular halotolerant cyanobacterium Aphanothece halophytica under nitrogen and potassium deprivation
(2023-01-06)
Chinchusak, Nattanon
;
Incharoensakdi, Aran
;
Phunpruch, Saranya
The unicellular halotolerant cyanobacterium Aphanothece halophytica is known as a potential hydrogen (H<inf>2</inf>) producer. This study aimed to investigate the enhancement of H<inf>2</inf> production under nutrient deprivation. The results showed that nitrogen and potassium deprivation induced dark fermentative H<inf>2</inf> production by A. halophytica, while no differences in H<inf>2</inf> production were found under sulfur and phosphorus deprivation. In addition, deprivation of nitrogen and potassium resulted in the highest H<inf>2</inf> production in A. halophytica due to the stimulation of hydrogenase activity. The effect of adaptation time under nitrogen and potassium deprivation on H<inf>2</inf> production was investigated. The results showed that the highest H<inf>2</inf> accumulation of 1,261.96 ± 96.99 µmol H<inf>2</inf> g dry wt−1 and maximum hydrogenase activity of 179.39 ± 8.18 µmol H<inf>2</inf> g dry wt−1 min−1 were obtained from A. halophytica cells adapted in the nitrogen- and potassium-deprived BG11 medium supplemented with Turk Island salt solution (BG11<inf>0</inf>-K) for 48 h. An increase in hydrogenase activity was attributed to the decreased O<inf>2</inf> concentration in the system, due to a reduction of photosynthetic O<inf>2</inf> evolution rate and a promotion of dark respiration rate. Moreover, nitrogen and potassium deprivation stimulated glycogen accumulation and decreased specific activity of pyruvate kinase. Transcriptional analysis of genes involved in H<inf>2</inf> metabolism using RNA-seq confirmed the above results. Several genes involved in glycogen biosynthesis (glgA, glgB, and glgP) were upregulated under both nitrogen and potassium deprivation, but genes regulating enzymes in the glycolytic pathway were downregulated, especially pyk encoding pyruvate kinase. Interestingly, genes involved in the oxidative pentose phosphate pathway (OPP) were upregulated. Thus, OPP became the favored pathway for glycogen catabolism and the generation of reduced nicotinamide adenine dinucleotide phosphate (NADPH), which resulted in an increase in H<inf>2</inf> production under dark anaerobic condition in both nitrogen- and potassium-deprived cells.
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Enhancement of dark fermentative hydrogen production in nitrogen-deprived halotolerant unicellular cyanobacterium Aphanothece halophytica by treatment with reducing agents
(2022-12-01)
Chinchusak, Nattanon
;
Incharoensakdi, Aran
;
Phunpruch, Saranya
To enhance H<inf>2</inf> production by the halotolerant unicellular cyanobacterium Aphanothece halophytica, effect of various kinds of reducing sugar and reducing agent on H<inf>2</inf> production was investigated. The highest H<inf>2</inf> production rate of 55.80 ± 0.50 μmol H<inf>2</inf> g dry weight−1 h−1 was obtained when the cells were incubated in BG11<inf>0</inf> medium containing 0.189 mmol C-atom L−1 glucose under dark anaerobic condition. This rate was 1.5 folds higher than that without glucose. Among ten reducing agents tested, β-mercaptoethanol, dithiothreitol, L-cysteine and sodium sulfide had high potential as an effective reducing agent to increase H<inf>2</inf> production by A. halophytica. Cells treated with 50 mM sodium sulfide showed the highest H<inf>2</inf> accumulation with 4815.59 ± 194.78 μmol H<inf>2</inf> g dry weight−1 after 24 h of dark anaerobic incubation. An increase in H<inf>2</inf> production was ascribed to an increase of hydrogenase activity and a decrease of O<inf>2</inf> production rate. This H<inf>2</inf> production yield was approximately 20 folds higher than that without reducing agent. Furthermore, 50 mM sodium sulfide appeared to be non-toxic to A. halophytica cells, since the IC<inf>50</inf> of sodium sulfide was higher than 100 mM. The reduced ferredoxin at 1.5 μM-1.5 mM, NADH and NADPH at 0.15–1.5 mM could support in vitro [NiFe]-H<inf>2</inf>ase activity, demonstrating the ability of these compounds to provide electrons towards [NiFe]-H<inf>2</inf>ase in A. halophytica.
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Light-Emitting Diode Illumination Enhances Biomass, Pigment, and Lipid Production in Halotolerant Cyanobacterium Aphanothece halophytica
(2025-06-01)
Thongtha, Sitthichai
;
Kittiwongwattana, Chokchai
;
Incharoensakdi, Aran
;
Phunpruch, Saranya
Light characteristics, including spectrum and intensity, significantly impact cyanobacterial biomass production, pigment biosynthesis, and cellular metabolism, influencing the composition of various biochemical compounds. This study aimed to investigate the effects of light-emitting diode (LED) illumination on biomass, pigment, and lipid production in the unicellular halotolerant cyanobacterium Aphanothece halophytica, cultivated in a suitable natural seawater (SNSW) medium. The results revealed that LED light outperformed fluorescent light, with blue LED light, particularly at an intensity of 60 μmol photons m−2 s−1, significantly enhancing growth, pigment synthesis, and lipid accumulation. This resulted in a maximum cell density of 68.96 ± 1.52 × 106 cells mL−1, a specific growth rate of 0.302 ± 0.002 day−1, and a lipid productivity of 56.81 ± 0.75 mg L−1 day−1. White LED light produced lipids suitable for biodiesel, whereas blue, green, and red LEDs promoted the accumulation of polyunsaturated fatty acids (PUFAs), beneficial for food supplements. These findings highlight the potential of LED-based cultivation strategies for optimizing biomass and biochemical compound production in A. halophytica.

Phunpruch, Saranya

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