Ruangsomboon, Suneerat
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Ruangsomboon, Suneerat
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suneerat.ru@kmitl.ac.th
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Item type:Publication, Enhancing biomass, hydrocarbon and biodiesel properties of green microalga Botryococcus braunii KMITL through gamma and UV radiation exposure(2024-12-01) ;Jongput, Buppha ;Chiwpreecha, Pattanasak; This study investigated the effects of gamma (<sup>137</sup>Cs, 0–250 Gy) and UV (UV-C, 0–12 h) radiation on growth and biodiesel properties of Botryococcus braunii KMITL. For gamma radiation, maximum biomass (1.37 ± 0.02 g L<sup>−1</sup>) was achieved with 50 Gy, while a dose of 200 Gy resulted in the highest hydrocarbon content (51.84 ± 0.20%) and yield (0.66 ± 0.01 g L<sup>−1</sup>). For UV radiation, a 9 h exposure produced the highest biomass (2.45 ± 0.05 g L<sup>−1</sup>), hydrocarbon content (55.01 ± 1.22%), and yield (1.35 ± 0.04 g L<sup>−1</sup>). Algae exposed to gamma radiation within the range of 0–150 Gy exhibited C16:0 as the dominant fatty acid methyl ester (FAME), similar to those exposed to UV radiation, while algae exposed to 200–250 Gy displayed C18:1n9t as the dominant FAME. High levels of gamma and UV radiation were observed to lengthen fatty acid chains and increase unsaturated fatty acids. The cetane values of biodiesel from algae exposed to gamma and UV radiation ranged from 64.55 ± 0.14–66.47 ± 0.20 and 59.43 ± 0.04–65.27 ± 0.22, respectively, all meeting standard criteria. Both gamma and UV radiation also improved the saponification value and cold flow properties of the biodiesel. These findings suggest that controlled levels of gamma and UV radiation effectively enhance hydrocarbon yields with significant implications for biofuel production. - Some of the metrics are blocked by yourconsent settings
Item type:Publication, Characterization and physicochemical properties of a novel microencapsulated bio-calcium from Asian sea bass bones(2025-12-01) ;Phengleng, Salinee; ; ; Calcium is the most abundant mineral in the human body, yet intake remains insufficient in many populations. Fishbone-derived bio-calcium from Asian sea bass (Lates calcarifer), containing approximately 37.5 % calcium (dry weight), offers a cost-effective source. However, its primary form, hydroxyapatite, has low solubility due to high crystallinity, limiting its application in food fortification. This study aimed to enhance the physicochemical properties of bio-calcium (B) powders by encapsulating them with maltodextrin (M), gum arabic (G), and their combination (MG) at 5 %, 10 %, and 15 % (w/v) using spray drying. A 1:4 (w/w) ratio of B to wall materials was applied at 180 °C (inlet) and 60 °C (outlet) temperatures. Powder yields ranged from 25.2 % (15 % BG) to 30.3 % (15 % BM), with no significant differences (p > 0.05) among treatments. Encapsulated powders had higher lightness (L*) than B. The highest calcium content and encapsulation efficiency were observed in 5 % BG, while BM showed the lowest. Moisture content and water activity remained below 10 % and 0.6 %, respectively. BG had the highest hygroscopicity, while wall concentration had no significant (p > 0.05) impact. Encapsulation improved water solubility index (75.4–86.5 %), especially in BM. Particle sizes ranged from 0.92 µm (10 % BMG) to 2.89 µm (15 % BM), while zeta potentials ranged from -8.71 mV (15 % BM) to -20.90 mV (15 % BMG). Encapsulated powders were more spherical and smoother than B, while BG particles showed aggregation, whereas BMG showed mixed morphologies. These findings suggest that encapsulation enhanced the physicochemical properties of bio-calcium, supporting its potential application in calcium-fortified foods and dietary supplements. - Some of the metrics are blocked by yourconsent settings
Item type:Publication, Cultivation of Wolffia globosa and its application in functional food development(2025-12-01); ; ;Jongput, Buppha; This study assessed the biomass productivity, biochemical composition, and functional food potential of Wolffia globosa cultivated in four nutrient media: AB hydroponic solution, Chlorella medium, 16-16-16 fertilizer, and 16-16-16 supplemented with a vitamin B complex and FeSO<inf>4</inf> (16-16-16-B). Cultivation was conducted for eight weeks, with biomass harvested weekly. Among the treatments, the Chlorella medium produced the greatest biomass yield (133.82–236.00 g FW tank⁻<sup>1</sup>) and daily productivity (23.90–42.14 g FW m⁻<sup>2</sup> day⁻<sup>1</sup>), significantly surpassing the other media (p < 0.05), followed by 16-16-16-B. Medium replenishment at four weeks initially enhanced growth but was later accompanied by a decline in biomass. Biochemical analysis indicated that the Chlorella medium yielded the highest protein content (46.10 ± 0.93% DW), while the AB medium supported the greatest chlorophyll-a (4.08 ± 0.00% DW) and carbohydrate levels (37.21 ± 1.12% DW); all differences among treatments were statistically significant (p < 0.05). Considering cost-effectiveness and nutritional value, the 16-16-16-B medium was optimal for cultivating W. globosa for food applications. Dried biomass from this medium was incorporated into fresh pasta and fried sweet potato balls, significantly enhancing chlorophyll-a, carotenoids, protein, fiber, and calcium contents. Sensory evaluation using a 9-point hedonic scale showed strong consumer acceptance even at higher inclusion levels. These findings highlight the role of nutrient-enriched media, particularly 16-16-16-B, in enhancing biomass yield and nutritional quality of W. globosa, affirming its potential as a functional food ingredient. Beyond nutritional enhancement, W. globosa-based foods exhibit immunomodulatory and anticancer potential through the antioxidant activity of pigments and other bioactive compounds. - Some of the metrics are blocked by yourconsent settings
Item type:Publication, Effect of CO2 Concentration on Growth, CO2 Fixation, and Biochemical Composition of the Microalga Nannochloropsis oculata(2022-03-01); Chonudomkul, DuenrutThe green marine microalga Nannochloropsis oculata is rich in biochemicals and well known as a suitable source for dietary supplements and biodiesel feedstock. This study investigated the effects of CO<inf>2</inf> supplementation on N. oculata in terms of its biomass, biochemical composition, CO<inf>2</inf> fixation, and biodiesel qualities. Microalgae were cultivated in f/2 medium supplied with CO<inf>2</inf> at 0.04-6%. The culture supplemented with 6% CO<inf>2</inf> produced the highest biomass (1.64±0.04 g L<sup>−1</sup>) and contents of carotenoids (0.93±0.00 µg g<sup>−1</sup>), lipids (47.68± 2.80%), eicosapentaenoic acid (EPA; 28.45 mg g<sup>−1</sup> biomass), and docosahexaenoic acid (DHA; 22.43 mg g<sup>−1</sup> biomass); it also demonstrated the highest CO<inf>2</inf> fixation rate (74.62±2.55 kg m<sup>−3</sup>y<sup>−1</sup>) and provided an algal oil with desirable biodiesel qualities, specifically high cetane (52.27-53.50) and low iodine (75.91-82.15 g I<inf>2</inf> 100 g<sup>−1</sup>) values meeting the biodiesel specifications established by standards in the US (ASTM D6751) and Europe (EN 14214). In sum, CO<inf>2</inf> supplementation during N. oculata cultivation enhanced its production of high-value biomolecules, making this algal species a good source for production of dietary supplements and biodiesel. - Some of the metrics are blocked by yourconsent settings
Item type:Publication, Outdoor open pond batch production of green microalga Botryococcus braunii for high hydrocarbon production: enhanced production with salinity(2020-12-01); ;Dimak, Jantra ;Jongput, Buppha ;Wiwatanaratanabutr, ItsanunKanyawongha, PornthiwaThe aim of this work was to enhance the biodiesel quality and hydrocarbon content of green microalga B. braunii strain KMITL 2 cultivated outdoor under several salinity conditions in a batch production. The enhancement would be such that the microalgal biodiesel qualities met or exceeded the current standard so that it would be a good raw material for biodiesel production. The microalga production was in 300 L open oval ponds, among various salinity levels tested (0–20 ppt), 5 ppt was the best for hydrocarbon production, yielding 54.2 ± 0.9% hydrocarbon content and 5.1 ± 0.4 g L<sup>−1</sup> biomass. As the microalga production was scaled up by cultivation in 3,675 L open raceway pond under the 5 ppt condition, the microalga yielded a bit higher hydrocarbon content (58.8 ± 2.9%) but much lower biomass (2.5 ± 0.5 g L<sup>−1</sup>). The production in both oval and raceway ponds yielded a nearly identical biodiesel property (61.06–67.42 cetane number) which exceeded the value specified in international standards. Therefore, it was concluded that B. braunii strain KMITL 2 can be batch cultivated in an open pond at optimum salinity to yield sufficient hydrocarbon and biomass for biodiesel production.
