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    Item type:Publication,
    Effects of Ya-nang leaves (Tiliacora triandra) powder on properties and oxidative stability of tilapia emulsion sausage during storage
    (2015-01-01)
    Sriket, P.
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    Effect of Ya-nang leaves (Tiliacora triandra) powder (YLP) (0.5-2.0%) on qualities and lipid oxidation of tilapia emulsion sausages during 21 days of refrigerated storage was investigated. Control sample had lower fiber content than samples added with YLP (p<0.05) at day 0 of storage. YLP treated samples (1.0-2.0%) revealed lower L* value but higher b* and ΔE* values, compared to the control samples (p<0.05). With the addition of YLP, peroxide value (PV) and thiobarbituric acid-reactive substances (TBARS) value in the sausages were retarded effectively, compared to control sample (p<0.05), especially when the YLP at high contents were used. Addition of YLP were also effective in retarding the formation of fishy odor in the samples, compared to control sample (p<0.05). Samples treated with YLP had higher hardness and springiness, compared with control sample during storage (p<0.05). YLP had no detrimental effect on the sensory attributes of sausages. This study pointed out that YLP can be utilized in sausages to enhance quality.
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    Item type:Publication,
    Antioxidant and anticancer activities on HT-29 colon cancer cells of protein isolate extracted from Cordyceps militaris fruiting body using diverse isolation methods
    (2024-11-01)
    Thirabunyanon, Mongkol
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    Mungmueang, Natthapong
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    Daengprok, Wichittra
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    Ngampeerapong, Chitraporn
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    Karnjanapratum, Supatra
    The present research examines different techniques for isolating proteins from Cordyceps militaris fruiting body powder (COR), namely ammonium sulfate precipitation (ASP), organic solvent precipitation (OSP), and isoelectric precipitation (IEP). ASP yielded the highest protein content (78.90 %), effectively concentrating proteins, while OSP and IEP also produced substantial yields (57.41 % and 69.06 %, respectively). Color analysis revealed differences among isolates, with ASP resulting in a darker hue. ASP and IEP showed higher red and yellow components, respectively. Essential amino acid content was significantly higher in ASP isolate compared to COR, indicating effective amino acid concentration. Thermal analysis revealed different decomposition temperatures and enthalpy values among isolates. OSP displayed the highest phenolic content, while IEP exhibited the highest flavonoid content. Antioxidant assays showed ASP to have the highest DPPH-RSA and ABTS-RSA, while IEP showed the highest FRAP value. ASP demonstrated anticancer activity against HT-29 colon cancer cells inducing cell death apoptosis and inhibiting cell migration, with an IC<inf>50</inf> of 360.63 µg/mL, comparable to Paclitaxel. These findings suggest that Cordyceps militaris protein isolate, particularly from ASP, has significant antioxidant and anticancer potential, supporting its potential as a natural alternative to synthetic drugs with fewer side effects.