Soytong, Kasem

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Soytong, Kasem
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Soytong, K.
Email
kasem.so@kmitl.ac.th

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    In vitro efficacy of Chaetomium brasiliense against Pythium spp. Causing root rot disease of tangerine
    (2018-12-01)
    Udompongsuk, M.
    ;
    Soytong, K.
    ;
    Kanokmedhakul, S.
    ;
    Kanokmedhakul, K.
    Root rot is one of the most serious disease in Tangerine which caused by Pythium spp. This study tested efficacy of Ch. brasiliense to control Pythium sp. by bi - culture, crude extracts and nano particles testing from Ch. brasiliense to control Pythium sp., in vitro. The results from the bi - culture testing, Ch. brasiliense inhibited mycelium growth and sporangia production by 42.50% and 48.41%, respectively. In crude extracts from Ch. brasiliense gave ED <inf>50</inf> values of 30.15, 58.71 and 37.25 ppm for the hexane, EtOAc and MeOH, respectively. The efficacy of nano particles against Pythium sp. with the ED <inf>50</inf> values of 2.69, 3.00 and 3.96 ppm for the hexane, EtOAc and MeOH, respectively.
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    Nano-particles derived from Chaetomium elatum against Phytophthora rot of durian
    (2018-12-01)
    Thongkham, D.
    ;
    Soytong, K.
    ;
    Kanokmedhakul, S.
    ;
    Kanokmedhakul, K.
    Durian are the economically fruit trees in Thailand. The important problem of durian is root rot disease caused by Phytophthora palmivora. This study was used Chaetomium elatum to control the P. palmivora causing root rot disease of durian by dual culture method, crude extract test and nano particles test derived from Ch. elatum. Dual-culture test showed that Ch. elatum gave efficiency to inhibit of spore and colony growth of P. palmivora which were 46.13 and 38.89%, respectively. Testing efficacy of crude extract from Ch. elatum to control P. palmivora found that crude ethyl acetate from Ch. elatum gave significantly highest against pathogen of P. palmivora at the concentration of 1000 ppm which the ED <inf>50</inf> of 175.31 ppm. Nano particles testing, nano particles of crude hexane, ethyl acetate and methanol from Ch. elatum showed the ED <inf>50</inf> values of 3.49, 3.47 and 3.41 ppm.
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    Nano-particles from Chaetomium brasiliense to control Phytophthora palmivora caused root rot disease in durian var Montong
    (2018-12-01)
    Tongon, R.
    ;
    Soytong, K.
    ;
    Kanokmedhakul, S.
    ;
    Kanokmedhakul, K.
    The antagonistic fungus, Chaetomium brasiliense was tested to control Phytophthora palmivora causing rot disease of Durian (Durio zibithenus L.) var Montong by crude extracts and Nano-particles derived from Ch. brasiliense. Crude extracts of antagonistic fungus was tested for antifungal biological activities. The crude extracts from antagonistic fungus with hexane, ethyl acetate and methanol were tested against P. palmivora. Crude ethyl acetate from Ch. brasiliense gave significantly against P. palmivora which the ED <inf>50</inf> values was 17.46 µg/ml. Testing Nano-particles were tested for antifungal activities. The results showed nano - particles from Ch. brasiliense gave effectively significantly inhibition of colony growth and spore production which the ED <inf>50</inf> values were 1.08 µg/ml, and 8.68 µg/ml respectively. Application of Nano - particles to control the P. palmivora causing root rot disease of durian in pot experiment was successfully done. The results showed nano-particles from Ch. brasiliense reduced the root rot disease on durain of 40%. The nano-particles from Ch. brasilense gave significiantly high plant growth which were 79.5 cm when compared to the non-trated control.
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    Nano-particles from Chaetomium lucknowense to inhibit rice blast pathogen causded by Pyricularia oryzae in pot experiment
    (2018-12-01)
    Song, J. J.
    ;
    Soytong, K.
    ;
    Kanokmedhakul, S.
    ;
    Kanokmedhakul, K.
    Nano-particles derived from Chaetomium lucknowense proved to be antagonized Pyricularia oryzae causing rice blast disease var. RD57. Result showed that nano-ECL, nano-MCL and nano-HCL expressed antifungal activities against P. oryzae (rice blast disease) at the ED <inf>50</inf> values were 82, 114, 181ppm, respectively. In pot experiment, the nano-CL gave significantly better to control rice blast than the chemical fungicide (Tricyclazole) in rice var. RD57. Rice blast disease showed that nano-CL gave the highest reduction of 54 %, when compared to the chemical fungicide that the disease decreased 29.26 %. Application of nano-CL gave the highest plant strands of 87.62 cm when compared to Tricyclazole (74.91 cm). Nano-particles from C. lucknowense is being developed to be nano-elicitors for plant immunity.
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    Nano-particles from Cheatomium brasiliense against brown spot of rice
    (2018-12-01)
    Vareeket, R.
    ;
    Soytong, K.
    ;
    Kanokmedhakul, S.
    ;
    Kanokmedhakul, K.
    Chaetomium brasiliense was used in this study to control rice brown spot disease pathogen, Drechslera oryzae. The result of bi-culture test showed that Ch. brasiliense gave the hightest percentage of growth inhibition at 26.38% and had the highest spore inhibition at 23.81%. In crude extracts test, it was found that crude methanol extract obtained from Ch. brasiliense at 1000 ppm showed the highest inhibitory effect on colony growth and spore production. Ch. brasiliense gave a growth inhibition and spore inhibition rates at 83.50 and 99.78% respectively. The effective dose (ED <inf>50</inf> ) on growth and spore inhibition of Ch. brasiliense was 80.54 and 0.35 µg/ml, respectively. It was also found that Nano-particles obtained from crude methanol extract of Ch. brasiliense (nano CBM) at 10 ppm had the best inhibitory effect in terms of growth and spore inhibition. Nano CBM can inhibit the growth at 70.00% and spore production at 79.92%. The ED <inf>50</inf> values for spore inhibition of Ch. brasiliense was 2.86 µg/ml.