Soytong, Kasem

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Effects of nanoparticles loaded with Chaetomium globosum KMITL-n0805 extracts against leaf spot of rice var. Sen pidoa
(2016-12-01)
Tann, Huyly
;
Soytong, Kasem
Nano-CGH, nano-CGE, and nano-CGM from Chaetomium globosum KMITL-N0805 expressed antifungal activity (ED<inf>50</inf> values of 1.21, 1.19, and 1.93ppm/mL, respectively) against Curvularia lunata, the causal agent of leaf spot disease of rice var. Sen Pidoa. It was demonstrated that these nanoparticles caused the disruption and distortion of the pathogen cells, leading to their loss of pathogenicity. Tests in a pot experiment showed that nano-CGH, nano-CGE, and nano-CGM could significantly control leaf spot of rice var. Sen Pidoa. Based on the disease severity index at 60 days after treatment, nano-CGH and nano-CGM resulted in higher disease reduction (61.54%) than nano-CGE (53.83%). From these findings, it can be concluded that nano-CGH, nano-CGE, and nano-CGM have the ability to decrease leaf spot disease of rice var. Sen Pidoa caused by C.lunata. Moreover, the research showed that all three types of nanoparticles significantly increased the height and number of tillers of the rice plant relative to the non-treated control.
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Integrated biological control of mango var. Choke Anan
(2000-01-01)
Noiaium, Sumitra
;
Soytong, Kasem
The project research is aimed to evaluate the biological products for controlling anthracnose of mango var. Choke Anan integrated with the other control measures in the field. Biological products formulated from Chaetomium (CC7+CG10) and Trichoderma (PC01+PC02) in the forms of pellets and suspension were tested in the field to control mango anthracnose caused by Colletotrichum gloeosporioides during 1996-1997. Results showed that the Chaetomium's pellet which broadcasting to rhizosphere soil at the rate of 20 g/plant every 4 months amended with 5 kg of organic compost could significantly reduce the pathogen inoculum and disease incidence of 79.88 and 55.93 percent, respectively. The plants treated with Trichoderma's pellets could also significantly reduce the pathogen inoculum and incidence of anthracnose of 81.26 and 55.53 percent, respectively when compared with the alternative spraying of the chemical fungicides such as Carbendazim, Zinep, Manep and Copper oxychloride which showed that the pathogen inoculum and disease incidence could reduce only 23.83 and 50.16 percent, respectively. Disease levels during flower setting until harvesting fruits were compared in the rainy (off-season fruits) and summer seasons. Results showed that in both seasons of the experiment, the plants treated with Chaetomium's pellets and spraying with the ascospore suspension of Chaetomium cupreum CC7 and C. globlosum CGIO at the rate of 22 × 10 <sup>10</sup> spore/ml in the same plants every month showed the same incidence of anthracnose on the fruits, as those plants treated with Trichoderma's pellet and spraying with spore suspension of Trichoderma harzianum PC01 and T. hamatum PC02 (404 × 10<sup>10</sup>) and those chemical fungicide treated ones. It was observed that the biological treatments gave better yield than the chemical fungicides treatment.
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Evaluation of bio-agent formulations to control Fusarium wilt of tomato
(2010-09-06)
Charoenporn, C.
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Kanokmedhakul, S.
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Lin, F. C.
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Poeaim, S.
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Soytong, K.
The ED<inf>50</inf> value of antagonistic substance from Chaetomium globosum N0802 extracted with hexane was 157 μg/ml. This gave the highest inhibition of conidial production of Fusarium oxysporum f. sp. lycopersici causing tomato wilt var Sida. Crude hexane from Chaetomium lucknowense CLT and crude methanol from Trichoderma harzianum PC01 gave ED<inf>50</inf> values of 188 and 192 μg/ml. It clearly demonstrated that antagonistic substances from all tested fungi could be deformed and this could break the conidial cells. The bio-agent formulations namely N0802, CLT and PC01 gave significantly highest disease reduction of tomato wilt which were 44.68, 36.28 and 41.01%, respectively, followed by prochoraz (21.95%). All tested bio-agent formulations could significantly increase the yield of tomato when compared to prochoraz and inoculated control. It is concluded that C. globosum, C. lucknowense and T. harzianum developed as bio-agent formulations namely N0802, CLT and PC01 and showed their abilities to control tomato wilt. © 2010 Academic Journals.
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Bioactive xanthoquinodins and epipolythiodioxopiperazines from Chaetomium globosum 7s-1, an endophytic fungus isolated from Rhapis cochinchinensis (Lour.) Mart
(2020-02-16)
Tantapakul, Cholpisut
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Promgool, Trinop
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Kanokmedhakul, Kwanjai
;
Soytong, Kasem
;
Song, Jiaojiao
A new xanthoquinodin B9 (1), together with two known xanthoquinodins, xanthoquinodin A1 (2) and xanthoquinodin A3 (3), three epipolythiodioxopiperazines, chetomin (4), chaetocochin C (5) and dethio-tetra(methylthio)chetomin (6), and four other compounds, chrysophanol (7), emodin (8), alatinone (9), and ergosterol (10) were isolated from the endophytic fungus Chaetomium globosum 7s-1, isolated from Rhapis cochinchinensis (Lour.) Mart. All isolated structures were established based on their spectroscopic data analyses. Compounds 1–6 showed antibacterial activity against Gram positive bacteria with MICs ranging from 0.02 pM to 10.81 µM. Compounds 1–6 also exhibited cytotoxicity against KB, MCF-7 and NCI-H187 cancer cell lines (IC<inf>50</inf> 0.04–18.40 µM). However, they were cytotoxic towards a normal cell line (Vero cell) with IC<inf>50</inf> values ranging from 0.04 to 3.86 µM.
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Soytong, Kasem

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