Maneeruttanarungroj, Cherdsak
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Maneeruttanarungroj, Cherdsak
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Email
cherdsak.ma@kmitl.ac.th
3 results
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Item type:Publication, INVESTIGATION OF ANTIFUNGAL ACTIVITY FROM SCENEDESMUS SP. AND CHLAMYDOMONAS SP. CRUDE EXTRACTS(2022-01-01); Scenedesmus sp. and Chlamydomonas sp. are green microalgae used for various biotechnology applications such as an alternative biofuel, antioxidant, human supplement, aquaculture feed etc. In a decade, finding a new antibiotic resource from a novel organism is significant for pharmaceutical research. Microalgae is a promising candidate since they consist of high-value biocompounds such as β-carotene, astaxanthin, flavonoids, polysaccharide. In this study, we aimed to preliminarily investigate antifungal activities of Scenedesmus and Chlamydomonas crude extracts against to Candida albicans, a fungal pathogen. We prepared both of intracellular and extracellular (culture media) crude extracts by using ethanol and methanol as a solvent and then tested C albicans inhibition performed by agar-well diffusion assay. From the preliminary results, we found that 50 mg/mL of Scenedesmus and Chlamydomonas ethanolic crude extracts from intracellular and extracellular could inhibit C. albicans. This preliminary finding could be a platform for a further study in some bio-based organic compounds that is significant for pharmaceutical application. - Some of the metrics are blocked by yourconsent settings
Item type:Publication, Effect of pH on Biohydrogen Production in Green Alga Tetraspora sp. CU2551(2017-01-01); The green alga Tetraspora sp. CU2551 has previously been identified as a hydrogen producing organism. With the promising potential in a high H<inf>2</inf> production yield, we further studied the enhancement of the H<inf>2</inf> production capacity by a substrate-level control to the key enzyme, Hydrogenase. This enzyme catalyzes the formation of biohydrogen gas from protons and electrons. Here, we investigated the effect of pH on the production rate which directly affected the flow of proton flux to the hydrogenase enzyme. The result revealed that pH of culture TAP medium was simply controlled by the use of HCl/NaOH solution over the pH range of 6.5 - 9.0 without any effect on the growth of algal cells. The H<inf>2</inf> production was observed in all ranges of our focusing pH with comparable to the control condition (pH 7.0 of TAP medium). In our best condition, an increase of the H<inf>2</inf> yield about 24% was found in cells cultivated in TAP medium adjusted the pH to 6.5 compared to the control condition. Our finding confirms the successful biohydrogen production enhancement by a substrate-level control for the key hydrogenase enzyme. - Some of the metrics are blocked by yourconsent settings
Item type:Publication, INVESTIGATION OF ANTIMICROBIAL CAPACITY AND ANTIOXIDANT EFFICIENCY FROM CHLORELLA SPP. CRUDE EXTRACTS(2023-01-01); Functional food, nutraceutical and pharmaceutical products are currently shared as an important part of the consumer market. Microalga plays a significant role in producing a source of good antioxidants including nutrient supplements such as chlorophyll, beta-carotene, astaxanthin etc. A Eukaryotic green microalga, Chlorella, is a unicellular microalga that itself is rich in a source of chlorophyll, carotenoids, the essential amino acids, bioactive compounds including fatty acids and these bioproducts make Chlorella as a great potential for human food and supplements, animal feed and biofuel feedstock. In this study, we aimed to test antimicrobial activity and antioxidant from four different Chlorella strains (Chlorella sp. KU11, Chlorella sp. V55, Chlorella sp. B2-59 and Chlorella sp. N11/59) for pharmaceutical and nutraceutical application. All four-ethanolic crude extracts of Chlorella were tested against six pathogens such as Streptococcus mutans ATCC25175, Bacillus subtilis ATCC6633, Staphylococcus aureus ATCC25923, Escherichia coli ATCC25922, Pseudomonas aeruginosa ATCC27853, and Candida albicans ATCC10231 using agar-well diffusion and the minimum inhibitory concentrating (MIC) assay. The result showed that 50 mg/mL of Chlorella sp. KU and Chlorella sp. V55 crude extracts could inhibit S. mutans, B. subtilis and S. aureus and 100 mg/mL of both crude extracts were able to inhibit E. coli, P. aeruginosa and C. albicans. From the minimum inhibitory concentrating (MIC) assay, we found that Chlorella sp. KU and Chlorella sp. V55 could inhibit S. mutans, B. subtilis, S. aureus E. coli, P. aeruginosa and C. albicans at 25, 50, 50, 100, 100 and 100 mg/mL, respectively and 50, 6.25, 25, 100, 100 and 100 mg/mL, respectively. The antioxidant activity of the four crude extracts were performed by DPPH radical scavenging assay and we found that the Chlorella sp. N11/59 crude extract showed the highest antioxidant efficiency with 63.73% and IC50 value was 2.8293 mg/mL. This finding suggested that microalgae can be a promising platform for pharmaceutical and nutraceutical application.