Wongsariya, Karn
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Preferred name
Wongsariya, Karn
Alternative Name
Wongsariya, K.
Main Affiliation
Email
karn.wo@kmitl.ac.th
7 results
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Item type:Publication, New Chitosan-Grafted Thymol Coated on Gold Nanoparticles for Control of Cariogenic Bacteria in the Oral Cavity(2022-08-02); ; Chitosan-grafted thymol (CST) coated on gold nanoparticles has been synthesized and characterized for the design of antimicrobial materials. CST was synthesized via adapting the Mannich reaction, and it acted as the capping agent for the synthesis of gold nanoparticles (AuNPs). The grafting of thymol onto the side chain of chitosan has provided a degree of substitution value (%DS<inf>NMR</inf>) of 10.0%, calculated by nuclear magnetic resonance spectroscopy. UV-visible spectrometry and elemental analysis were used to confirm the successful synthesis of CST through adapting the Mannich reaction. The appropriate concentration of CST for AuNP synthesis was found to be 0.020%w/v. A red-wine colloidal AuNP solution of 2.41-3.30 nM particle size exhibits a strong surface plasmon resonance at 502 nm, which shows negative charges at pH = 9 of -36.37 mV. This result evidenced that the AuNPs showed electrostatic repulsion and CST played a role as a capping agent to provide a good dispersion and stability state. CST coated on the AuNP surface was successfully utilized for the control of cariogenic bacteria in the oral cavity. The results obtained from this study show that the tuning of the capping agent used in the synthesis step strongly influences the latter antimicrobial activity of the nanoparticles against Streptococcus mutans ATCC 25175 and Streptococcus sobrinus ATCC 33402 activity, with an inhibition zone of 15.90 and 14.25 mm, respectively. The average minimum inhibitory concentration values against S. mutans ATCC 25175 and S. sobrinus ATCC 33402 were found to be 25 and 100 mg/L, respectively, whereas the minimum bactericidal concentration values were 100 and 200 mg/L, respectively. - Some of the metrics are blocked by yourconsent settings
Item type:Publication, Characteristics and upregulation of antioxidant enzymes of kitchen mint and oolong tea kombucha beverages(2021-01-01); Kombucha is a healthy traditional beverage which is made by fermenting products with a symbiotic culture of acetic acid bacteria and yeasts. In present study, leaves of kitchen mint (Mentha cordifolia Opiz. Ex Fresen) and leaves of oolong tea (Camellia sinensis) were fermented in kombucha formula. After fermentation, titratable acidity contents and ethanol of kitchen mint, oolong tea, and mixtures of oolong tea and kitchen mint kombucha samples gradually increased with a period of fermentation time. At day 14 of fermentation, phenolic compounds and flavonoids were increased in all kombucha samples. The numbers of acetic acid bacteria and yeast in kombucha had gradually raised during 7–14 days of fermentation. DPPH and ABTS scavenging activities of these kombucha increased over a period of fermentation time and shown the highest antioxidant capacity on day 14 of fermentation. In addition, all kombucha samples exhibited the antioxidant effects by attenuating H<inf>2</inf>O<inf>2</inf>-induced ROS production, increasing mRNA expression of catalase, glutathione reductase (GRe), and Mn-SOD, and inducing GRe enzymatic activity in HEK-293 cells. Kombucha beverage can be used as the healthy beverages for attenuation of oxidative stress in many diseases. - Some of the metrics are blocked by yourconsent settings
Item type:Publication, Proximate composition of the edible part of purple passion fruit and santol and in vitro prebiotic activity of crude polysaccharide extracts(2021-06-01); Kanchanadumkerng, P.This study aimed to evaluate the functional and prebiotic properties of the edible portions of certain tropical fruits in Thailand, passion fruit and santol. The proximate composition of passion fruit and santol were analysed which total carbohydrate was the major component in both samples. Total dietary fibre of passion fruit and santol was 44.81 and 26.82% (w/w), respectively, with the majority of insoluble dietary fibre. Soluble dietary fibre of both fruits was extracted by hot water and water-extractable polysaccharide was yielded at 16.47% and 25.24% (w/w) for passion fruit and santol, respectively. The functional properties of fruit fibre were informed, with the satisfactory oil holding capacity of santol polysaccharide. The effect of both fruit polysaccharides on proliferation number at 24 hrs was not different from inulin. Prebiotic activity score of fruit polysaccharide was calculated from the growth of Lactobacillus acidophilus and Bifidobacterium longum compared with the growth of the enteric pathogen, Escherichia coli corresponding to-0.25 and-0.23 for passion fruit and 0.10 and-0.01 for santol, respectively. In conclusion, both passion fruit and santol polysaccharide showed a distinct effect on the supportive growth of probiotic bacteria which may be potential candidate ingredient incorporated in probiotic food. From this evidence, the development of fruit-based synbiotics from passion fruit and santol migh be affordable. - Some of the metrics are blocked by yourconsent settings
Item type:Publication, Lactobacillus plantarum JCM 1149 Growth Enhancement by using Chlorella sp. KLSc61-pretreated Cells(2025-06-01); ; Dry microalgal biomass was previously tested as prebiotic to enhance the growth of probiotic bacteria. However, the drying process could be ineffective for scaling up probiotic production. This study aimed to investigate the use of fresh, pretreated microalgal biomass to promote the growth of Lactobacillus plantarum JCM 1149. Chlorella sp. KLSc61 cells were pretreated by three different methods: physical treatment with microwave radiation at power levels of 300, 500, and 700 W; chemical treatment with 0.1 M citric acid and 0.5 M sodium hydroxide; and biological treatment with cellulase enzyme. The 2.5% pretreated Chlorella cells were then added to L. plantarum JCM 1149 culture, and the growth was observed at 37 °C for 24 h of incubation. The results showed that, during the log phase (6-10 h), Chlorella cells pretreated with microwave radiation at 700 W were the most effective in promoting L. plantarum JCM 1149 growth, which was 1.4- and 1.5-fold of L. plantarum JCM 1149 without adding Chlorella and with untreated cells, respectively. Extension of the pretreatment time by microwave radiation at 700 W from up to 2 min increased the growth of L. plantarum JCM 1149 up to 1.8-fold of pretreatment time by microwave radiation at 700 W 1 min, compared to the control groups. Additionally, increasing the amount of Chlorella biomass up to 5% (w/v) extended the log phase of L. plantarum JCM 1149 and increased cell accumulation during the stationary phase. Unlike dry microalgal biomass, the simplicity of fresh, pretreated Chlorella biomass shown in this study may facilitate large-scale, commercial production of L. plantarum strains as probiotics. - Some of the metrics are blocked by yourconsent settings
Item type:Publication, Effects of natural sugar on acidogenic potential, biofilm biomass, and antiseptic resistance of oral streptococci(2021-01-01) - Some of the metrics are blocked by yourconsent settings
Item type:Publication, Genome Characterization for the Antimicrobial Potential of Streptomyces samsunensis SA31, a Rhizospheric Actinomycete of Cymbopogon citratus (DC) Stapf.(2025-01-01); ; - Some of the metrics are blocked by yourconsent settings
Item type:Publication, Anti-oral streptococci and anti-biofilm properties of Etlingera pavieana essential oil and its bioactive compounds proposed for an alternative herbal mouthwash(2024-05-30);