Srirussamee, Kasama

Preferred name

Srirussamee, Kasama

Main Affiliation

School of Engineering

kasama.sr@kmitl.ac.th

Now showing 1 - 10 of 13

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Electroactive 3D printed scaffolds based on percolated composites of polycaprolactone with thermally reduced graphene oxide for antibacterial and tissue engineering applications
(2020-03-01)
Angulo-Pineda, Carolina
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Srirussamee, Kasama
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Palma, Patricia
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Fuenzalida, Victor M.
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Cartmell, Sarah H.
Applying electrical stimulation (ES) could affect different cellular mechanisms, thereby producing a bactericidal effect and an increase in human cell viability. Despite its relevance, this bioelectric effect has been barely reported in percolated conductive biopolymers. In this context, electroactive polycaprolactone (PCL) scaffolds with conductive Thermally Reduced Graphene Oxide (TrGO) nanoparticles were obtained by a 3D printing method. Under direct current (DC) along the percolated scaffolds, a strong antibacterial effect was observed, which completely eradicated S. aureus on the surface of scaffolds. Notably, the same ES regime also produced a fourfold increase in the viability of human mesenchymal stem cells attached to the 3D conductive PCL/TrGO scaffold compared with the pure PCL scaffold. These results have widened the design of novel electroactive composite polymers that could both eliminate the bacteria adhered to the scaffold and increase human cell viability, which have great potential in tissue engineering applications.
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Microstructure and mechanical properties of Ti-Zr alloys fabricated by two-step spark plasma sintering from TiH2 and ZrH2 powders
(2023-01-01)
Banjongaxsorn, Kunpot
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Khantachawana, Anak
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Watanabe, Chihiro
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Srirussamee, Kasama
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Kondoh, Katsuyoshi
Ti is well-known for its high strength-to-weight ratio and biocompatibility. It can be alloyed with Zr to improve mechanical properties. Currently, Ti-Zr alloys can be fabricated by powder metallurgy using Ti and Zr powders, although high oxidation reactivity and cost could still be an issue. In this study, premixed TiH<inf>2</inf> and ZrH<inf>2</inf> powders were used to prepare Ti-Zr binary alloys with different Zr contents of 0–30 mass% ZrH<inf>2</inf> via dehydrogenation and sintering by spark plasma sintering (SPS) process. α-Ti and δ-TiH<inf>2</inf> phases coexisted in the sintered Ti-Zr alloys, and a characteristic lamellar microstructure was formed. The tensile strength of the Ti-Zr alloys increased with increasing Zr content due to the solid solution effect, grain refinement and the appearance of δ-TiH<inf>2</inf> phases, although the elongation was reduced. This study shows that the fabricated Ti-Zr alloys possess controllable mechanical properties, which can be beneficial for biomedical and other engineering applications.
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Changes in the extracellular microenvironment and osteogenic responses of mesenchymal stem/stromal cells induced by in vitro direct electrical stimulation
(2021-01-01)
Srirussamee, Kasama
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Xue, Ruikang
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Mobini, Sahba
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Cassidy, Nigel J.
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Cartmell, Sarah H.
Electrical stimulation (ES) has potential to be an effective tool for bone injury treatment in clinics. However, the therapeutic mechanism associated with ES is still being discussed. This study aims to investigate the initial mechanism of action by characterising the physical and chemical changes in the extracellular environment during ES and correlate them with the responses of mesenchymal stem/stromal cells (MSCs). Computational modelling was used to estimate the electrical potentials relative to the cathode and the current density across the cell monolayer. We showed expression of phosphorylated ERK1/2, c-FOS, c-JUN, and SPP1 mRNAs, as well as the increased metabolic activities of MSCs at different time points. Moreover, the average of 2.5 μM of H<inf>2</inf>O<inf>2</inf> and 34 μg/L of dissolved Pt were measured from the electrically stimulated media (ES media), which also corresponded with the increases in SPP1 mRNA expression and cell metabolic activities. The addition of sodium pyruvate to the ES media as an antioxidant did not alter the SPP1 mRNA expression, but eliminated an increase in cell metabolic activities induced by ES media treatment. These findings suggest that H<inf>2</inf>O<inf>2</inf> was influencing cell metabolic activity, whereas SPP1 mRNA expression was regulated by other faradic by-products. This study reveals how different electrical stimulation regime alters cellular regenerative responses and the roles of faradic by-products, that might be used as a physical tool to guide and control cell behaviour.
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Characterization of oxide films on wrought Co–Cr–Mo–xSi alloys exposed to high-temperature oxidation
(2021-10-01)
Tunthawiroon, Phacharaphon
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Kitiwan, Mettaya
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Srirussamee, Kasama
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Li, Yunping
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Yamanaka, Kenta
Co-based alloys are currently being used in a wide range of high temperature applications owing to their high resistance to oxidation and corrosion. However, their oxidation-induced degradation could still occur during the long-term exposure to high temperature. Thus, the continuous development of oxidation-resistant Co-based alloys is of crucial importance. In this research, the influence of Si addition on the oxidation behavior of Co–Cr–Mo–xSi alloys under the isothermal oxidation treatment at 700 °C in air was investigated. The Si concentration (x) was varied from 0.1 to 5.0 wt.%. Surface morphologies and chemical compositions of the oxide films formed were analyzed by using SEM-EDS and XPS. The chemical compositions obtained from the surface analysis revealed that Si has played a role in the stabilization of Cr oxides on the surface of Co–Cr–Mo–xSi alloys. With increasing Si concentration, Co-oxide formation on the alloy surface was suppressed by the presence of Cr-oxide due to the selective oxidation of Cr atoms. Furthermore, SiO<inf>2</inf> was both found along the grain boundaries and interfaces between the outmost oxide layer and matrix. It was also found that the oxide thickness was reduced with increasing Si concentration. This was a result of the formation of stable Cr-oxide and SiO<inf>2</inf> sub-layer that became a barrier inhibiting the inward and outward diffusion of O and Cr.
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Assessment of the utility of chitosan nanoparticles and microfibers in drug delivery applications of sulfamethoxazole and ciprofloxacin
(2025-05-07)
Soontorntepwarakul, Nussara
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Boonyarattanakalin, Kanokthip
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Fukasem, Poowadon
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Somkhuan, Sitthinan
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Srirussamee, Kasama
The objective of this research was to explore the suitability of chitosan (CS) in the form of nanoparticles (NP) and microfibres (MF) for oral delivery applications related to low solubility antibiotic drugs sulfamethoxazole and ciprofloxacin. The ionic gelation method in conjunction with freeze-drying was used to produce crosslinked chitosan material. The dynamic light scattering (DLS) technique was used to characterize the particle size and polydispersity index (PDI). Surface morphology was analyzed using scanning electron microscopy (SEM). The antibiotic drugs ciprofloxacin (CPF) and sulfamethoxazole (SMO) were loaded onto the chitosan nanoparticle and microfibre materials. The degree of loading and the release kinetics were investigated using high-performance liquid chromatography (HPLC) and a UV-visible spectrophotometer, respectively. CS itself has mucoadhesive properties and combined with the more rapid release of drug compared to the pure API, or traditional tablet formulations, CS nanoparticles and microfibres have the potential to improve the delivery properties of CPF and also SMO to a lesser extent. This difference can be rationalized on account of their differing physicochemical properties.
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Comparative study of vacuum arc-remelting and spark plasma sintering processes on microstructure and corrosion behavior of Cp-Ti for biomedical implant applications
(2025-11-01)
Kunbuala, Neeraphat
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Srirussamee, Kasama
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Phamornnak, Chinnawich
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Tunthawiroon, Phacharaphon
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Hankoy, Montree
Titanium (Ti) and its alloys are widely used for biomedical applications due to their excellent mechanical properties and biocompatibility. However, the selection of an appropriate manufacturing process is critical to ensuring the optimal performance of Ti-based implants. This study investigates the effects of two fabrication methods –vacuum arc remelting (VAR) and spark plasma sintering (SPS) – on the microstructure and corrosion behavior of commercially pure titanium (Cp-Ti). VAR-Ti ingots were fabricated using arc-melting with multiple remelting cycles, whereas SPS-Ti specimens were sintered from Ti powders under pressure and pulsed current in a high-vacuum environment. Both specimens were subsequently heat-treated at 800 °C and furnace cooled. Microstructural characterization revealed coarser grains and porosity in VAR-Ti, while SPS-Ti showed refined, uniform α-phase structures. Electrochemical tests, including OCP, polarization, EIS, and ICP-MS, indicated slightly enhanced corrosion resistance in SPS-Ti, attributed to its defect-free microstructure. XPS analysis confirmed TiO<inf>2</inf> surface formation on both samples. Additionally, both materials exhibited high ductility and excellent biocompatibility, with cell viability exceeding ISO 10993-5 thresholds. These findings highlight the advantage of SPS in producing defect-minimized Cp-Ti with improved corrosion behavior for biomedical applications.
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Thermomechanical performance of the offset crankshaft heat engine driven by tinicu shape memory alloys
(2021-01-01)
Srirussamee, Kasama
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Khantachawana, Anak
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Hok, Bunheng
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Phukaoluan, Aphinan
Geothermal hot springs are among the alternative clean energy sources to the fossil fuels for mitigating the current global warming crisis. However, the accessible geothermal water at the surface was mostly at low temperature, which impairs the practicality of harvesting these energy. Shape memory alloys (SMAs), which deform through the increased temperature, were adapted into the rotating mechanism as the actuators with the aims to convert the low-temperature heat into the mechanical work. This study utilized the helical spring-shaped TiNiCu SMAs as the actuators for the offset crankshaft heat engine. Performance of this engine was evaluated using rope brake dynamometer, by which the rotational speed, torque, and power were measured at the water temperature from 55-85°C. The results show that the engine performance increased with increasing water temperature and was dependent on the crankshaft arrangement. The offset angle of 30° was found to be optimal in this study with maximum torque of more than 5.8 N∙m and maximum power of 3.9 W at 15.7 rpm when operating at water temperature of 85°C. This study shows that the heat engine driven by TiNiCu SMAs could harvest low-temperature energy from the geothermal hot springs with the maximum observable efficiency of around 1.4%.
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Co-stimulation with piezoelectric PVDF films and low intensity pulsed ultrasound enhances osteogenic differentiation
(2025-08-01)
Tandon, Biranche
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Aguilar Cosme, Jose R.
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Xue, Ruikang
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Srirussamee, Kasama
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Aguilar-Tadeo, Julio
Bone tissue engineering has emerged as a promising approach to address the challenges of bone fracture repair and regeneration. The application of external stimuli (mechanical and electrical) can drive specific cellular responses and osteogenic differentiation, leading to the development of more effective treatments. Piezoelectric materials modulate cellular proliferation and osteogenic differentiation under both static (without mechanical stimulation) and dynamic (with mechanical stimulation) conditions, activating distinct gene expression pathways. In this work, we investigate the combinatorial effect of poly (vinylidene fluoride) (PVDF) poled and non-poled films, and low-intensity pulsed ultrasound (LIPUS) on early-stage osteogenic differentiation of mouse pre-osteoblasts. Static culture with PVDF poled films enhanced Runx2 and Col1α1 expression without impacting alkaline phosphatase (ALP) activity. Inhibition of ERK phosphorylation using U0126 in PVDF poled films resulted in a ~ 6–8-fold increase in ALP activity, suggesting the involvement of an alternative pathway in osteogenic differentiation. Dynamic culture with LIPUS generated an electric potential of approximately 500 mV across PVDF films and an electrical field of 0–10 mV mm<sup>−1</sup>. Co-stimulation led to a ~3-fold increase of ALP activity on stimulated PVDF compared to unstimulated films. This study underscores the potential of piezoelectric materials as non-invasive electrical stimulators to enhance the efficacy of ultrasound-based therapies for bone fracture repair.
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Antibacterial characterization of ciprofloxacin-doped electrospun of low molecular weight polyethylene oxide (PEO) and sodium alginate (NaAlg) nanofibers
(2026-02-23)
Niyomchon, Phuphinee
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Treebupachatsakul, Treesukon
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Srirussamee, Kasama
Producing nanofibers using the electrospinning technique is a developed method that is widely used and of significant interest nowadays. This technique can be applied using various types of polymers. This research aimed to investigate the antibacterial PEO-NaAlg nanofiber fabrication. The fiber fabrication was examined under various viscosities of electrospinning solution. The electrospun nanofiber fabrication focuses on blending polyethylene oxide (PEO) with a molecular weight of 200-300 kDa, mixed with sodium alginate (NaAlg) of three different viscosities: 150 cP, 300 cP, and 730 cP to study how the viscosity of the solution affects the morphology of electrospun nanofibers. The PEO-NaAlg electrospun nanofiber was enhanced for water insolubility by crosslinking with calcium chloride (CaCl₂). The additional antibacterial property of the nanofiber by loading an antibacterial agent potentially against the growth of bacteria, was investigated. Antibacterial drug, ciprofloxacin at varying amounts of 0.05%w/v, 0.20%w/v, and up to 0.25%w/v was loaded to PEO-NaAlg solution and conducted electrospinning. The effectiveness of the antibacterial electrospun nanofiber was evaluated by testing its ability to inhibit the growth of Escherichia coli (E. coli) and Staphylococcus aureus (S. aureus). The inhibition area before and after crosslinking was observed. The results showed that the acquired nanofibber formation required 7%w/v of 200 kDa to 300 kDa of PEO, and blending 1%w/v NaAlg of 150 cP can certainly retain fiber morphology after crosslinking. Moreover, nanofibers loaded with ciprofloxacin effectively inhibit the growth of E. coli.
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Influence of mouthwash rinsing on the mechanical properties of polymeric ligature ties used for dental applications
(2021-07-02)
Phukaoluan, Aphinan
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Khantachawana, Anak
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Dechkunakorn, Surachai
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Anuwongnukroh, Niwat
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Tunthawiroon, Phacharaphon
Mouthwashes are used during dental treatments to mitigate the complications caused by poor oral hygiene. However, these solutions also affect the properties of dental appliances, including those used in orthodontics. This point has been investigated in this study focusing on the changes in mechanical properties of polymeric orthodontic ligature ties. Commercial ties from four brands were characterized in terms of their maximum forces and displacement, delivery forces, molecular structures, and microscopic morphology. These properties were compared against the ties, which were rinsed with commercial mouthwashes from three manufacturers. The results showed that mouthwash rinsing significantly reduced the maximum bearable forces of ligature ties by up to 73.1%, whereas the reduction in their maximum displacement was up to 74.5% across all tested brands. Significant changes in microscopic morphology of ligature ties were observed after mouthwash rinsing, but not their molecular structure. Furthermore, mouthwash rinsing also reduced the delivery forces from ligature ties by between 20.9 and 32.9% at their first deformation cycle. It can be concluded from this study that mouthwashes have significant impact on the mechanical properties of polymeric orthodontic ligature ties and could also potentially affect the overall efficacy of orthodontic and other dental treatments.

Srirussamee, Kasama

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