Chutipaijit, Sutee

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Preferred name
Chutipaijit, Sutee
Alternative Name
Chutipaijit, S.
Main Affiliation
Email
sutee.ch@kmitl.ac.th

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    Item type:Publication,
    Biodiversity and bioactivity of stromatic xylariales collected from Mu Ko Chang National Park, Thailand
    (2020-01-01)
    Sutjaritvorakul, T.
    ;
    Chutipaijit, S.
    Ko Chang Island is a tropical rain forest island of Mu Ko Chang national park, Thailand, which has a wide variety of macro-fungi. The objective of this research was to study the biodiversity of Xylariales and to investigate the bioactive activity of ethyl acetate crude extracts. The fungi were collected from the forest area of the waterfalls all over the island. The crude extracts of selected fungi were tested for antibacterial activity by agar well diffusion method and the cancer cell viability was assessed using the MTT assay. Three genera of Xylariaceae and Hypoxylaceae were found including Xylaria, Daldinia and Hypoxylon. The crude extracts inhibited the growth of Gram positive bacteria more than that of Gram negative bacteria and showed broad spectrum inhibition to all cancer cell lines. The crude extract of Xylaria sp.3 showed the highest potential of antibacterial activity and selectively inhibited gastric carcinoma (Kato III) and T cell leukemia (Jurket) with the percentage survival of 26.12 ± 0.90% and 35.37 ± 0.40% respectively. The molecular identification of Xylaria sp.3 was identified as Xylaria sp.TP5BS101. These results show that these macromycetes are an excellent repertoire of antibacterial activity and exhibit anticancer activity against Kato III and Jurket by inducing DNA fragmentation.
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    Item type:Publication,
    GREEN SYNTHESIS OF ZINC OXIDE NANOPARTICLES FROM LEMON PEEL EXTRACT AND THEIR ANTIBACTERIAL ACTIVITY
    (2024-01-01)
    Wirunchit, Supamas
    ;
    Sakulpeeb, Natchayaporn
    ;
    Chutipaijit, Sutee
    ;
    Nukaew, Jiti
    ;
    Koetniyom, Wantana
    This research project aimed to study the extraction of flavonoids from lemon peels using different solvents, namely deionized water (DI water), isopropanol, ethanol, and methanol. Among these, DI water yielded the highest amount of flavonoids. The extracted compounds were then utilized for the synthesis of zinc oxide nanoparticles (ZnO NPs) through a green chemical method. Various amounts of lemon peel extract (10, 15, 20, 25, and 30 ml) were mixed with Zn(NO3)2 during the synthesis process. The ZnO NPs were characterized using UV-VIS spectrophotometry, X-ray diffractometry (XRD), Fourier transform-infrared spectrometry (FT-IR), and Raman spectrometry. The results revealed successful synthesis of ZnO NPs, except for those produced using DI water extract, which exhibited a different structure (wurtzite). Subsequently, the antibacterial properties of the ZnO NPs were tested against Staphylococcus aureus (S. aureus) and Escherichia coli (E. coli) using the disc diffusion method. The ZnO NPs synthesized from different solvent extracts demonstrated effective inhibition zones against both bacterial strains, indicating their potential as antibacterial agents.