Parinthawong, Nonglak
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Preferred name
Parinthawong, Nonglak
Alternative Name
Parinthawong, N.
Main Affiliation
Email
nonglak.pa@kmitl.ac.th
12 results
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Item type:Publication, Micromonospora endophytica sp. nov., an endophytic actinobacteria of Thai upland rice (Oryza sativa)(2015-11-01); ; ; An actinobacterial strain, DCWR9-8-2 T, was isolated from a leaf of Thai upland rice (Oryza sativa) collected in Chumporn province, Thailand. Strain DCWR9-8-2 T is Gram-stain-positive aerobic bacteria that produce single spores directly on the vegetative hypha. Cell wall peptidoglycan of this strain exhibits meso-diaminopimelic acid and glycine, the reducing sugars of whole-cell hydrolysate are arabinose, glucose, ribose, xylose and small amount of mannose. The phospholipid profiles in the membrane are comprised of phosphatidylethanolamine, diphosphatidylglycerol, phosphatidylglycerol, phosphatidylinositol, phosphatidylinositol mannosides. The major menaquinones are MK-9(H 4) and MK-10(H 6). The diagnostic cellular fatty acids are iso-C 16:0 and iso-C 15:0. The G+C content of the genomic DNA is 72.5 mol%. The result of 16S rRNA sequence analysis of the strain revealed that this strain was closely related to Micromonospora auratinigra TT1-11 T (99.25%). On the other hand, the result of gyrB gene sequence analysis revealed that this strain was closed to M. eburnea JCM 12345 T (96.30%). In addition, a combination of DNA-DNA hybridization results and some phenotypic properties supported that this strain should be judged as a novel species of the genus Micromonospora, for which the name M. endophytica sp. nov. is proposed. The type strain is DCWR9-8-2 T (=BCC 67267 T =NBRC 110008 T). - Some of the metrics are blocked by yourconsent settings
Item type:Publication, Isolation and expression analysis of the Flavanone 3-Hydroxylase genes in Lotus (Nelumbo nucifera gaertn.), Waterlily (Nymphaea sp.) and transient silencing in Waterlily(2017-04-01); - Some of the metrics are blocked by yourconsent settings
Item type:Publication, Tissue culture and transformation of the antisense DFR gene into lotus (Nelumbo nucifera Gaertn.) through particle bombardment(2013-09-24); ; Arunyanart, SumayThe effects of explant and plant growth regulators on callus induction and plant regeneration and particle bombardment-induced transformation were examined in lotus (Nelumbo nucifera Gaertn.) cv. Buntharik. The apical buds from embryos (~3. mm) formed an embryogenic callus when cultured on MS medium supplemented with 40. μM 1-napthaleneactic acid (NAA) and 0.5. μM 1-phenyl-3-(1,2,3-thiadiazol-5-yl)-urea (TDZ) for 8 weeks. All of the embryogenic calluses were transferred to MS medium supplemented with 0, 40, 50 and 60. μM 6-benzyladenine (BA) and the highest number of shoots was achieved in the medium supplemented with 50. μM BA after 8 weeks of culture. The transformation of lotus using a particle bombardment device was examined with the shoot clusters from embryogenic apices. The pCAMBIA2301anti-DFR plasmid contained β. -glucuronidase (GUS) as a reporter gene, neomycin phosphotransferase (NPTII) as a selectable marker, and antisense dihydroflavonol 4-reductase (anti-. DFR) as the gene of interest. The particle bombardment procedure with a helium gas pressure of 1100. psi and a target distance of 6. cm yielded the highest number of blue spots (4.8 spots/shoot cluster). However, stable transformations, as confirmed by polymerase chain reaction and reverse transcription polymerase chain reaction analysis, were only achieved using a helium gas pressure of 1100. psi and a target distance of 9. cm. Our work illustrates that the stable transformation of lotus is possible, and we suggest that this methodology can be used to target and modify specific charactics of this plant. © 2013 Elsevier B.V. - Some of the metrics are blocked by yourconsent settings
Item type:Publication, Mating type and genetic diversity analysis of Pyricularia oryzae collected from Thai rice varieties(2018-12-01) - Some of the metrics are blocked by yourconsent settings
Item type:Publication, Micromonospora oryzae sp. Nov., isolated from roots of upland rice(2015-11-01); ; ; An actinomycete strain, designated CP2R9-1<sup>T</sup>, was isolated from root internal tissues of upland rice (Oryza sativa). Based on a polyphasic approach, strain CP2R9-1<sup>T</sup> was characterized as a member of the genus Micromonospora. meso- Diaminopimelic acid and 3-OH-diaminopimelic acid were present in the cell-wall peptidoglycan. The polar lipids were diphosphatidylglycerol, phosphatidylethanolamine, phosphatidylglycerol, phosphatidylinositol, phosphatidylinositol mannosides, two unidentified phospholipids and four unidentified polar lipids. Predominant menaquinones were MK-9(H<inf>4</inf>), MK-9(H<inf>6</inf>) and MK-10(H<inf>4</inf>). Whole-cell sugars consisted of ribose, xylose, arabinose and glucose. Phylogenetic analysis of the nearly complete 16S rRNA gene sequence suggested that strain CP2R9-1<sup>T</sup> was closely related to Micromonospora haikouensis 232617<sup>T</sup> (99.32% similarity), Micromonospora carbonacea DSM 43168<sup>T</sup> (99.18%) and Micromonospora krabiensis MA-2<sup>T</sup> (99.16%). Strain CP2R9-1<sup>T</sup> was distinct from its closest relatives based on low levels of DNA–DNA relatedness (21.3¡0.1– 41.7¡0.7%) and phenotypic differences. The results presented in this study showed that strain CP2R9-1<sup>T</sup> represents a novel species of the genus Micromonospora, for which the name Micromonospora oryzae sp. nov. is proposed. The type strain is CP2R9-1<sup>T</sup> (5BCC 67266T5NBRC 110007<sup>T</sup>). - Some of the metrics are blocked by yourconsent settings
Item type:Publication, Phytoalexin production of lettuce (Lactuca sativa L.) grown in hydroponics and its in vitro inhibitory effect on plant pathogenic fungi(2017-09-01); Jaenaksorn, TanimnunA series of experiments were conducted to investigate phytoalexin production from five varieties of lettuce in hydroponics using abiotic (2.5, 5% CuSO<inf>4</inf>; 0.5, 1% AgNO<inf>3</inf>) and biotic elicitors (non-pathogenic Pythium sp.) at different plant ages. It showed that phytoalexin was successfully induced in tested lettuce grown in hydroponics after elicitation with abiotic elicitors throughout the trial. Phytoalexin showed yellow fluorescent spot under 365 nm UV light with R<inf>f</inf> 0.45-0.48 and clear inhibition zone where Aspergillus niger failed to develop on TLC plate. For biotic elicitors, no yellow fluorescent spot on TLC plate was observed from tested lettuce varieties however inhibition zone at R<inf>f</inf> 0.9 was detected at 8-9 weeks from red oak, green oak, and red coral. Moreover, crude extract of lettuce elicited with abiotic elicitors possessed in vitro antifungal activity against C. gloeosporioides, C. lunata, F. oxysporum, and P. aphanidermatum probably due to phytoalexin (lettucenin A) in the extract. - Some of the metrics are blocked by yourconsent settings
Item type:Publication, Micromonospora soli sp. nov., isolated from rice rhizosphere soil(2016-03-01); ; ; An actinomycete strain SL3-70<sup>T</sup> was isolated from a rice field and characterised using a polyphasic approach. The morphological and chemotaxonomical characteristics of strain SL3-70<sup>T</sup> indicate that it belongs to the genus Micromonospora. The phylogenetic analysis of the nearly complete 16S rRNA gene sequence revealed that strain SL3-70<sup>T</sup> is a member of the genus Micromonospora, and is closely related to Micromonospora echinaurantica DSM 43904<sup>T</sup> (99.1 % 16S rRNA gene sequence similarity) and Micromonospora kangleipakensis MBRL 34<sup>T</sup> (98.8 %). DNA–DNA relatedness between strain SL3-70<sup>T</sup> and its relatives ranged from 21.2 % ± 0.6 to 38.7 % ± 0.4. The results obtained from our study indicate that strain SL3-70<sup>T</sup> represents a novel species of the genus Micromonospora, for which the name Micromonospora soli sp. nov. is proposed. The type strain is SL3-70<sup>T</sup> (=BCC 67268<sup>T</sup>; =NBRC 110009<sup>T</sup>). - Some of the metrics are blocked by yourconsent settings
Item type:Publication, Transformation of antisense dihydroflavonal 4-reductase (DFR) into sacred lotus 'Buntharik' using Agrobacterium-mediated gene transfer(2014-03-13); ; Arunyanart, S.Transformation of antisense dihydroflavonal 4-reductase (DFR) into 'Buntharik' sacred lotus (Nelumbo nucifera Gaertn.) using Agrobacterium-mediated gene transfer was experimented. The A. tumefaciens strain EHA105 harbored two binary vectors. The pCAMBIA2301anti-DFR plasmid contained neomycin phosphotransferase (NPTII) as a selectable marker gene, the antisense DFR as an inserted gene and the β-glucuronidas gene (GUS) as a reportor gene. The pBI121anti-DFR plasmid contained NPTII gene and the antisense DFR. Twomonths-old calli regenerated from apical buds were used as plant materials. The plant materials were soaked in the Agrobacterium suspension for 10 or 30 min. The calli were co-cultivated for two days in the darkness. Treated calli were selected on solid MS medium containing 50 mg L<sup>-1</sup> kanamycin and 250 mg L<sup>-1</sup> cefotaxime. The calli were transferred to the same medium every two weeks for eight weeks. The survived calli regenerated shoots on MS medium containing a combination of 0.54 μM NAA and 4.44 μM BA. Both plasmids gave the best callus growth, maximum callus size and the survival percentage when soaked for 10 min. GUS bioassay was used to verify the presence of GUS gene in petioles and leaves of transgenic plants. It was found that petioles and leaves of transformants which were transformed with pCAMBIA2301anti-DFR by soaking for 30 min showed the highest percentage of blue spots on explants. Of all 14 PCR positive clones, 5 clones showed GUS gene, 11 clones contained NPTII gene and 6 clones had DFR gene. - Some of the metrics are blocked by yourconsent settings
Item type:Publication, Genetic mapping of leaf blast resistance gene in landrace rice cultivar ‘GS19769’(2015-08-31); - Some of the metrics are blocked by yourconsent settings
Item type:Publication, Identification of blast-resistant varieties from Landrace, improved and wild species of rice(2013-07-18)