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    Item type:Publication,
    Nano-particles from Chaetomium lucknowense to inhibit rice blast pathogen causded by Pyricularia oryzae in pot experiment
    (2018-12-01) ; ;
    Kanokmedhakul, S.
    ;
    Kanokmedhakul, K.
    Nano-particles derived from Chaetomium lucknowense proved to be antagonized Pyricularia oryzae causing rice blast disease var. RD57. Result showed that nano-ECL, nano-MCL and nano-HCL expressed antifungal activities against P. oryzae (rice blast disease) at the ED <inf>50</inf> values were 82, 114, 181ppm, respectively. In pot experiment, the nano-CL gave significantly better to control rice blast than the chemical fungicide (Tricyclazole) in rice var. RD57. Rice blast disease showed that nano-CL gave the highest reduction of 54 %, when compared to the chemical fungicide that the disease decreased 29.26 %. Application of nano-CL gave the highest plant strands of 87.62 cm when compared to Tricyclazole (74.91 cm). Nano-particles from C. lucknowense is being developed to be nano-elicitors for plant immunity.
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    Item type:Publication,
    Natural product of nano-particles constructed from chaetomium spp. To control rice blast disease caused by magnaporthe oryzae
    (2020-01-01) ; ;
    Kanokmedhakul, Somdej
    ;
    Kanokmedhkul, Kwanjai
    ;
    Nanoparticles containing active compounds derived from Chaetomium cochliodes (CTh05) was tested to control rice blast disease caused by Magnaporthe oryzae isolate PO1. The causal agent of rice blast on leaves of rice var. RD57 was isolated. M. oryzae and C. cochliodes were identified morphologically and confirmed by molecular phylogenetic. M. oryzae was demonstrated to be pathogenic, causing blast of rice var. RD57. Biculture tests demonstrated that C. cochliodes could suppress the growth of M. oryzae. The crude metabolites of C. cochliodes (CCoH, CCoE and CCoM) at the concentrations of 10-1000 ppm expressed antifungal activity against the M. oryzae, resulting in inhibited spore production in 12 days with effective dose (ED<inf>50</inf>) values of 85, 144 and 374 ppm, respectively. The nanoparticles sizes of nano-CCoH, nano-CCoE and nano-CCoM were ranged between 567-611, `422-566 and 415-472 nm, respectively. Nanoparticles derived from C. cochliodes (nano-CCoH, nano-CCoE and nano-CCoM) at concentrations of 3-15 ppm significantly inhibited M. oryzae in 12 days with ED<inf>50</inf> values of 9, 16 and 33 ppm, respectively. In vivo experiments revealed a reduction of 38% in blast disease after application of nanoparticles constructed from crude extract mixtures from C. cochliodes at 10 ppm for 30 days. Tricyclazole resulted in reduction of blast disease by 29%. Rice blast disease was decreased in 30 days after applying nano-CCoM at the concentration of 7 ppm (60% disease reduction), followed by nanoCCoE and nanoCCoH with disease reduction of 58 and 50% respectively, and tricyclazole resulted in a 56% reduction in rice blast.