Tangthirasunun, Narumon
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Preferred name
Tangthirasunun, Narumon
Alternative Name
Tangthirasunun, N.
Main Affiliation
Email
narumon.ta@kmitl.ac.th
9 results
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Item type:Publication, Application of organic soybean tempeh in purple sweet potato raisin cookie(2022-05-01); Organic soybean tempeh or tempeh kedelai is made by fermentation with Rhizopus oligosporus was used to produce cookies, as a plant-based protein snack. Four samples with various recipes - purple sweet potato cookie, purple sweet potato raisin cookie, tempeh purple sweet potato cookie and tempeh purple sweet potato raisin cookie – were prepared to determine the physicochemical and sensory features. Chemical analysis found that the tempeh purple sweet potato raisin cookie has the highest moisture (18%) and total proteins (29%). However, the purple sweet potato cookies showed the highest ashes content (2%) and tempeh purple sweet potato cookie showed the highest total lipids (29%). The tempeh purple sweet potato raisin cookie was hardest (61 N). The tempeh purple sweet potato raisin cookie had the highest scores for food acceptability of all qualities in appearance, texture, aroma, colour, taste and general acceptability using a 9-point hedonic scale ranging from slightly liked to very much liked. Therefore, the tempeh purple sweet potato raisin cookie can be accepted as a new snack, that includes a good level of protein and thus a healthy food alternative. Furthermore, if all steps of the production are carefully controlled by USDA or other certifying bodies, thus this product may be labelled as organic product. - Some of the metrics are blocked by yourconsent settings
Item type:Publication, studies on the Rapid and simple DNA extraction Method, Antibacterial Activity and enzyme Activity involved in Plant Biomass Conversion by Cookeina sulcipes and C. tricholoma (Cup Fungi)(2022-12-01); Cookeina sulcipes and C. tricholoma are a cup fungi (Ascomycota) collected in saraburi, thailand. the fungi have been isolated, cultured and confirmed as respective species. For morphology, both Cookeina sp. are white mycelium and the growth rate on potato dextrose agar (PDA) result present C. sulcipes is faster than C. tricholoma. the molecular characterization from a rapid and simple DNA extraction method that is modified based on thermolysis method, the DNA extraction is finish in thirty minutes and efficiency to continuous with polymerase chain reaction (PCR) amplification to fungi species level identification. the DNA sequence from internal transcribed spacer (its) gene regions by universal primer pairs its5/its4 is effective to confirm Cookeina species level that C. sulcipes has 616 bp and C. tricholoma has 570 bp. including, DNA sequence of large subunit (lsU) gene regions by universal primer pairs lROR/lR5 is generate that C. sulcipes has 912 bp and C. tricholoma has 906 bp. the cultures are screened for antibacterial activity by agar plug diffusion method and found that both isolates have been no activity against test strains (Bacillus subtilis, Escherichia coli, Kocuria rhizophila (Micrococcus luteus), Pseudomonas aeruginosa, Staphylococcus aureus and S. epidermidis). in a preliminary screening test of enzymes involved in plant biomass breakdown by agar plate method, both Cookeina sp. show cellulolytic and hemicellulolytic enzymatic activity, and manganese peroxidase (MnP) productivity. in contrast, only C. sulcipes had additional laccase activity. Neither isolate generate pectinolytic and lignin peroxidase (liP) activities. thus, Cookeina spp. proved the potentiality to break down lignocelluloses. - Some of the metrics are blocked by yourconsent settings
Item type:Publication, Evaluation of genetic diversity by molecular markers in Indian gooseberry (Phyllanthus emblica)(2025-01-01); - Some of the metrics are blocked by yourconsent settings
Item type:Publication, Genetic characterization of cassava (Manihot esculenta Crantz) cultivars using ISSR molecular marker(2024-05-01); - Some of the metrics are blocked by yourconsent settings
Item type:Publication, Isolation, screening wood rot fungi from the tropical forest of Thailand and their lignocellulolytic enzyme production under solid-state fermentation using agricultural waste as substrate(2025-01-01); The present study aimed to discover good lignocellulolytic enzyme (LCE) producers from Thailand’s tropical forest and then examine their multiple LCE production (including carboxymethyl cellulase (CMCase), xylanase, and laccase) using agricultural wastes as substrate. The total collection was 50 fungi, mainly from the Polyporales, Agaricales, and Xylariales orders. During primary screening by qualitative method and secondary screening by quantitative method, two potential fungi were proposed for multiple LCE production, including Auricularia auricula-judae 088 and Pseudolagarobasidium acaciicola TDW-48. Under solid-state fermentation (SSF) using agricultural wastes as substrates, P. acaciicola TDW-48 performed as a good producer that highly secreted simultaneous CMCase, xylanase, and laccase. In the next stage, the simplex lattice mixture design assessed the interaction of agricultural waste substrates and their effects on P. acaciicola TDW-48’s enzyme production. The results indicated that agricultural waste has different influences on CMCase, xylanase, and laccase production: orange peel showed a positive effect on both CMCase and xylanase activity, but a negative effect on laccase. In contrast, wheat bran positively influenced laccase, while it limited CMCase and xylanase. However, the combination of these substrates in the mixture showed synergic effects and improved enzyme activity. Through numerical optimization, a ternary mixture of wheat bran (1.27 g), orange peel (1.53 g), and rice husk (0.2 g) was identified as the most appropriate formulation for simultaneous multiple LCE production, reaching 20.96 U/g substrate for CMCase, 23.94 U/g substrate for xylanase, and 27.55 U/g substrate for laccase. These results provided a promising candidate for LCE production with high applicability in lignocellulose bioconversion and successfully demonstrated the relationship between the agricultural waste substrate and multiple LCE production that supported the enzyme production following the environmentally friendly and economical approach. - Some of the metrics are blocked by yourconsent settings
Item type:Publication, Isolation and Characterization of Xylanase from a Novel Strain, Penicillium menonorum SP10(2023-01-01); Xylanase has been applied in various sectors, such as biomass conversion, paper, pulp, textiles, and pharmaceutical industries. This study aimed to isolate and screen potential xylanase-producing fungi from the soil of Suphan Buri Province, Thailand. Fifteen fungi were isolated, and their xylanase activities were tested by the qualitative method. The result showed that isolate SP3, SP10 and SP15 gave high xylanase activity with potency index (PI) of 2.32, 2.01 and 1.82, respectively. These fungi were selected for the xylanase quantitative test, isolate SP10 performed the highest xylanase activity with 0.535 U/mL. Through molecular methods using the β-tubulin gene, isolate SP10 was identified as Penicillium menonorum. The xylanase characteristics from P. menonorum SP10 were determined, including the xylanase isoforms and the optimum pH and temperature. The xylanase isoforms on SDS-PAGE indicated that P. menonorum SP10 produced two xylanases (45 and 54 kDa). Moreover, its xylanase worked optimally at pH 6 and 55 °C while reaching 61% activity at 65 °C. These results proposed P. menonorum SP10 as a good candidate for industrial uses, especially in poultry feed and pulp industries, to improve yield and economic efficiency under slightly acidic and high-temperature conditions. - Some of the metrics are blocked by yourconsent settings
Item type:Publication, Antibacterial and lignocellulose-degrading enzyme activities of coprophilous fungi obtained from cow dung in Thailand(2024-01-01); - Some of the metrics are blocked by yourconsent settings
Item type:Publication, Assessing genetic diversity of Yellow Star tree using SRAP markers and efficacy of their endophytic fungi in biological control(2023-05-01); ; - Some of the metrics are blocked by yourconsent settings
Item type:Publication, Effective of Neosatorya to control Phomopsis asparagi causing stem blight of asparagus(2018-12-01); ; The biocontrol of stem blight of asparagus Phomosis asparagi was proved by Neosartorya and Talaromyces. The symptoms are appearance as oval-shaped lesions with light brown centres and slightly darker margins. Ten isolates of pathogen were isolated by tissue transplanting technique and confirmed by morphology and molecular identification based on internal transcribed spacer (ITS)-nrDNA sequence as P. asparagi. Fungal mycelia showed undulate margins and colonies white to gray and reached a diameter of 9 cm petri dish after 10 days. Alpha conidia were oblong or spindle-shaped, with a size of 2‒3.5 × 6.5‒8.8 µm. The fungal pathogenicity test showed the asparagus was infected by P. asparagi within 2 days on moisture chamber at room temperature. Neosartorya and Talaromyces were proved antifungal acrtivety against P. asparagi causing of stem blight of asparagus in dual culture. It revealed that N. hiratsuka EU06 was the highest inhibition P. asparagi causing the stem blight of asparagus at 59.50%.