Sompornpailin, Kanokporn

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Sompornpailin, Kanokporn
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kanokporn.so@kmitl.ac.th

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    High contents of proline and anthocyanin increase protective response to salinity in Oryza sativa L. spp. indica
    (2011-09-01)
    Chutipaijit, Sutee
    ;
    Cha-um, Suriyan
    ;
    Sompornpailin, Kanokporn
    Pigmented rice cultivar is a good candidate for abiotic tolerance especially salt tolerant trait. Physiological and growth parameters of eight indica rice genotypes under control or salt stress conditions were subjected to Hierarchical cluster analysis. From cluster ranking, rice genotypes were classified into two groups, salt-tolerant (Sangyod, Khao Dang, Kulab Dang and TD49) and salt-sensitive genotypes (Klum Sakol, Klum Khonkaen1, Klum Khonkaen2 and Black Sticky Rice). In salt stress (100 mM NaCl), the salt-tolerant genotypes showed the high percentages of proline and anthocyanin more than in the salt-sensitive genotypes. In addition, the photosynthetic pigment stabilizations of stressed-seedlings were positively correlated to the proline (R <sup>2</sup> = 0.98) and anthocyanin (R <sup>2</sup> = 0.70) accumulations. While, the malondialdehyde (MDA) contents of salt-stressed seedlings were negatively correlated to the proline (R <sup>2</sup> = 0.63) and anthocyanin (R <sup>2</sup> = 0.51) accumulations. Moreover, the total antioxidant activity of extraction from stressed-seedlings was assessed using the ABTS (2,2-azino-bis-3-ethylbenzothiazoline-6-sulfonic acid) method. The higher percentages of total antioxidant activity were determined in salt-tolerant (125-199%) than salt-sensitive genotypes (106-113%). From the results it can be concluded that accumulation of above mentioned metabolites is associated with cellular protection and salt detoxification in salinity rice seedlings.
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    Differential accumulations of proline and flavonoids in indica rice varieties against salinity
    (2009-10-01)
    Chutipaijit, Sutee
    ;
    Cha-Um, Suriyan
    ;
    Sompornpailin, Kanokporn
    Four varieties of indica rice differing in salt sensitivity were used for comparative study of defense systems in response to salinity. Rice seedlings were transferred into the photoautotrophic system containing NB medium with 100 mM NaCl as a salt stress treatment for 4 days. The physiological responses of jasmine rice (KDML105) and Sangyod (SY) varieties showed the better tolerance to salinity than those of Pathumthani 1 (PT1) and Black Sticky (BS) varieties. In salt stress treatment, the relative water content (RWC) of rice seedlings was decreased when compared to untreated seedlings. The RWC of salt-tolerant seedlings (KDML105 and SY) were decreased (0.33-0.53%) less than those of salt-sensitive seedlings (PT1 and BS), which exhibited 1.18-2.63% reductions. The salt-tolerant KDML105 and SY showed the lesser extent of membrane damage (lipid peroxidation; 7.94-19.26%) while enhanced the proline level (56.56-78.56%) and the flavonoid level (1.72-3.48%) more than those of the sensitive PT1 and BS varieties. Moreover, the positive correlation between proline or flavonoid accumulations and RWC was observed whereas the correlation between proline or flavonoid accumulations and MDA content was negative. These results suggest that high proline and flavonoids levels accumulated in each varieties of rice might be closely related to tolerant abilities indicating by physiological performances.
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    Enhancement of plant regeneration efficiency from mature grains of thai indica rice (Oryza sativa L. cv. KDML105)
    (2012-08-01)
    Sompornpailin, Kanokporn
    ;
    Chutipaijit, Sutee
    In vitro protocol for efficient plant regeneration has been developed from Thai indica rice cultivar (Oryza sativa L. cv. KDML105). Embryogenic callus was initiated from mature seeds on NBMI medium. The highest frequency (96.00%) of callus induction was obtained on NBMI1 medium [2 mg L-1 2,4-dichlorophenoxyacetic acid (2,4-D) without kinetin]. These calli were sub-cultured on NBM medium supplemented with various concentrations of BA or combination with supporting materials for plant regeneration. Plant regeneration from embryogenic callus was increased in the medium supplemented with 5 g L-1 Phytagel® in the regeneration medium (NBMR9 medium; 30.00%). In addition, the best regenerated frequency in this experiment was obtained by culturing the embryogenic callus to 4:4 ratios of Gro-lux tubes to fluorescent light tubes at 1000 lux light intensity (56.72%). The regenerated plants were successfully transplanted to soil. The results indicated that manipulation of medium supplements and the cultured condition leads to increase plant regeneration efficiency in KDML105 cultivar for genetic transformation in the future.
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    Proline accumulation and physiological responses of indica rice genotypes differing in tolerance to salt and drought stresses
    (2010-06-01)
    Chutipaijit, Sutee
    ;
    Suriyan, Cha Um
    ;
    Sompornpailin, Kanokporn
    The study determined the relationship between proline accumulation and physiological responses of indica rice genotypes to salt and drought stresses in photoautotrophic conditions. Two salt-tolerant genotypes of indica rice [cv. jasmine rice (KDML 105) and Dang], two drought-tolerant genotypes [Rice Department 6 (RD6) and Rice Department 15 (RD15)] and a salt-and drought-sensitive genotype [Pathumthani 1 (PT 1)] were exposed to 100 mM NaCl (salt stress) or 100 mM mannitol (drought stress) for 4 d. The relative water content (RWC) and malondialdehyde (MDA) accumulation in salt-and drought-tolerant genotypes did not significantly change. The proline accumulation in these genotypes greatly increased with stress. On the other hand, the salt-and drought-sensitive genotype which had lower proline accumulation demonstrated a significant decrease in RWC and increase in MDA content. Moreover, our results showed a strong correlation between proline concentrations and free radical scavenging by the 2,2-diphenyl-1-picrylhydrazyl (DPPH) assay. The high concentration of proline solution presented high DPPH antioxidant activity. The higher proline accumulation in stressed seedlings of tolerant genotypes may help to maintain the RWC and MDA of rice plant, which could lead to preferable growth performance.
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    An evaluation of water deficit tolerance screening in pigmented indica rice genotypes
    (2012-02-01)
    Chutipaijit, Sutee
    ;
    Cha-Um, Suriyan
    ;
    Sompornpailin, Kanokporn
    Eight pigmented genotypes of indica subspecies were geminated and then treated by mannitol-induced water deficit stress. A change of growth characteristics, photosynthetic pigments, lipid peroxidation, DNA content, proline content and anthocyanin accumulation in stressed seedling (100 mM mannitol) and control plant (0 mM mannitol) were calculated. Growth performances, photosynthetic pigment concentrations, and DNA contents in all rice genotypes were dropped whereas proline, anthocyanin contents and the lipid peroxidation levels were enriched. The stabilization in total photosynthetic pigment concentrations of stressed-seedlings were positively correlated to the proline or anthocyanin accumulation. In contrast, MDA content, the increases in the percentages of drought-stressed seedlings were negatively correlated to the proline or anthocyanin accumulation. The changes in biochemical, physiological and growth parameters were subjected to Ward's cluster analysis for water deficit tolerance. These cultivars could be classified into two groups, water deficit sensitive, SY, KD, KLD and TD49 and water deficit tolerance, KS, KK1, KK2 and BSR.