Montri, Nattaya

Preferred name

Montri, Nattaya

Alternative Name

Montri, N.

Main Affiliation

School of Agricultural Technology

nattaya.mo@kmitl.ac.th

Now showing 1 - 10 of 14

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Influence of geographical and genetic variation on GABA content in Annona muricata grown in Thailand
(2020-12-08)
Deewatthanawong, R.
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Tontiworachai, B.
;
Kongchinda, P.
;
Chanapan, S.
;
Sriwilaiwan, S.
Annona is a genus of tropical fruit trees in the family Annonaceae. Fruit pulp of Annona muricata contained the highest amount of gamma-aminobutyric acid (GABA) among Annona species. GABA content results from many factors including genetic makeup and environment. Influences of genetic variation and geographical environment on GABA content in fruit pulp of A. muricata were determined. To study genetic variation, sequences of ITS2 and psbA-trnH were used to construct phylogenetic trees. Young leaves of A. muricata were collected from different geographical locations including Chumphon, Trang, Nakhon Ratchasima, Nakhon Si Thammarat, Buriram, Nong Khai, Udon Thani, and Prachuap Khiri Khan Provinces in Thailand. Sequences of ITS2 and psbA-tmH were aligned and data sets were analyzed using maximum likelihood (ML) and neighbor-joining (NJ) methods. ITS2 sequence variations were observed within and among samples from different locations. A. muricata fruit harvested from eight provinces, namely Chumpon, Trang, Nakhon Ratchasima, Nakhon Si Thammarat, Buriram, Nong Khai, Udon Thani, and Prachuap Khiri Khan contained 19.75, 35.02, 32.91, 28.18, 42.37, 32.17, 65.02, and 31.58 mg 100 g-1 FW GABA, respectively. Findings indicated that both geographical environment and genetic variation had significant effects on GABA content in A. muricata fruit.
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The effect of auxins on root production and stemona alkaloid accumulation in Stemona curtisii Hook.f. in vitro culture
(2023-02-01)
Montri, N.
;
Saenpakdee, S.
;
Deewatthanawong, R.
The herb Stemona curtisii Hook.f. has insecticidal properties. Its roots contain a specific group of stemona alkaloids. This study evaluated the effect of auxins on root production and stemona alkaloid accumulation in roots of S. curtisii. In vitro shoots were cultured on MS medium (Murashige and Skoog, 1962) supplemented with various types of auxins as indole-3-acetic acid (IAA), indole-3-butyric acid (IBA) and α-naphthalene acetic acid (NAA) at concentrations of 0, 0.2, 0.4, 0.6, 0.8 and 1.0 mg L-1 for 2 months. Results showed that auxins enhanced both fresh and dry root yields and also improved alkaloid contents compared to non-treated roots. Adding 0.6 mg L-1 IBA achieved highest root yield at 19.50% and stemocurtisinol content at 23.831×10-6 mg g-1 DW, while the highest total alkaloids and stemocurtisine contents were found in 0.8 mg L-1 IBA treatment at 186.976×10-3 mg g-1 DW and 340.142×10-6 mg g-1 DW, respectively.
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Development of transient expression system for gene functional analysis in Setaria viridis
(2022-04-01)
Deewatthanawong, R.
;
Kamoltham, M.
;
Montri, N.
An efficient transient expression system to enable functional gene analysis was developed in Setaria viridis, a new C4 model plant. A promoter deletion analysis of C4 phosphoenolpyruvate carboxylase (PEPC) was used to demonstrate the protoplast transient expression system mediated by polyethylene glycol (PEG). Protoplasts were isolated from young leaves of S. viridis. A series of promoter deletion fragments ranging in size from 243 to 859 bp were fused to firefly luciferase and co-transfected with Renilla luciferase. Luciferase levels were used to determine promoter activities based on the ratio of firefly/Renilla to normal cell number and transfection efficiency. Luciferase activities of the longer PEPC promoter constructs exhibited higher promoter activities. Results revealed potential uses of the protoplast transient expression system for promoter analysis in S. viridis.
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Production of F1 Papilionanthe hookeriana (Rchb.f.) Schltr. homozygous and heterozygous: Amplified fragment length polymorphism (AFLP) analysis of flower colors and self and cross-pollination ability between different flower colors
(2023-11-01)
Tontiworachai, B.
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Deewatthanawong, R.
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Intasam, N.
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Buamas, S.
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Promvai, S.
Results showed that seed germination of Papilionanthe hookeriana (Rchb.f.) Schltr. was not significantly different between self-pollination treatments, while the percentage of selfpollination ability and seedling numbers were significantly different at 0.01 and 0.05 levels, respectively. Pink and purple flowers had the highest self-pollination ability at 100% and the highest numbers of seedlings (1,295) for self-pollination of pink flowers. Percentages of crosspollination ability, pod size and weight, and seed weight were not significantly different between treatments, while percentages of seed germination and seedling numbers were significantly different at the 0.01 level. The PixPu hybrid showed 100% seed germination, whereas the PixWh hybrid yielded the highest number of seedlings (403.2). Amplified fragment length polymorphism (AFLP) technique was used to study the genetic relations between various flower colors of P. hookeriana (Rchb.f.) Schltr. and the F1 hybrids of self- and cross-pollinated plants were classified into two clusters by UPGMA methods.
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Effect of plant growth regulators on in vitro seedling growth of Papilionanthe hookeriana (Rchb.f.) Schltr.
(2023-02-01)
Montri, N.
;
Charoensrimuang, S.
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Deewatthanawong, R.
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Montri, W.
;
Bunya-Atichart, K.
The effect of plant growth regulators on the in vitro seedling growth of Papilionanthe hookeriana (Rchb.f.) Schltr. was evaluated. The protocorms were cultured in VW media supplemented with combinations of indole acetic acid (IAA) and thidiazuron (TDZ) at the concentrations of 0, 0.1 and 0.2 mg L-1. The 2 leaf stage seedlings were cultured on VW media supplemented with cytokinins; 6-furfurylaminopurine (kinetin: K), benzyladenine (BA) and TDZ or auxins; IAA, indole-3-butyric-acid (IBA), α-naphthalene acetic acid (NAA) at the concentrations of 0, 2 and 4 mg L-1 for 2 months. The results found that combinations of IAA and TDZ had higher protocorm fresh weight when compared with control. Adding with 0.1 and 0.2 mg L-1 IAA alone were promoted protocorm fresh weight. On the other hand, combinations of 0.1 mg L-1 IAA and 0.2 mg L-1 TDZ also improved the fresh weight number. The combinations of TDZ and IAA treatments were achieved for the number of 1st stage protocorm. For 2 leaf stage seedling culture, treated with cytokinins was not significantly different in shoot length, seedling fresh weight and leaf number. However, cytokinins suppressed root number compared to untreated. The maximum root numbers at 4.21 roots were found in free cytokinins medium. Seedling fresh weight and leaf numbers were significantly increased when increased the concentration of auxins. Our results indicated that auxins and cytokinins played an instrumental role for P. hookeriana seedling growth.
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Effect of calcium phosphate on growth, pigment and secondary compound accumulation in Cannabis sativa L. callus
(2026-05-01)
Tebdoie, C.
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Thipphaaut, T.
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Deewatthanawong, R.
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Kongchinda, P.
;
Singhavorachai, P.
The application of calcium phosphate in the form of tricalcium phosphate (Ca3(PO4)2) on cannabis (Cannabis sativa L.) callus cultures for 7 days significantly increased fresh and dry weights at concentrations ranging from 800 to 2000 mg/L, with no statistical differences between concentrations. However, higher calcium phosphate concentrations led to reductions in pigments and DPPH radical scavenging activity. By contrast, callus cultures treated with 800 and 1600 mg/L showed enhanced total phenolic content and carotenoids, while total flavonoid content increased proportionally. These findings suggested that calcium phosphate concentration influenced the biosynthesis of secondary metabolites in cannabis callus cultures.
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Non-destructive measurement of Tetrahydrocannabinol (THC) and Cannabidiol (CBD) using near-infrared spectroscopy
(2023-11-01)
Deewatthanawong, R.
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Kongchinda, P.
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Chanapan, S.
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Tontiworachai, B.
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Sakkhamduang, C.
Tetrahydrocannabinol (THC) and cannabidiol (CBD) are cannabinoids which produced by cannabis plants and major compounds found in cannabis products. A predictive method for non-destructive quantification of THC and CBD using near infrared spectroscopy (NIR) technology is developed. The prediction model for THC estimation had coefficient of determination (R-squared) and root mean square error of calibration (RMSEC) values of 0.9994 and 0.1926, respectively. The correlation between THC values of HPLC measurement and NIR prediction showed a correlation coefficient of 0.9078. For CBD prediction, the R-squared and RMSEC values of CBD equation were 0.9995 and 0.0006, respectively. The predicted and measured concentrations of CBD showed good correlation with a regression correlation of 0.9413. The test indicated NIR could be a promising alternative method for THC and CBD evaluation.
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Phytochemical analysis of Melaleuca cajuputi Powell leaves for the development of herbal tea
(2026-06-01)
Montri, N.
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Chalornsiri, S.
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Pato, N.
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Kueasakun, N.
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Deewatthanawong, R.
The plant Melaleuca cajuputi Powell, commonly known as cajuput, is found in coastal swamp forests in Thailand. Traditional knowledge suggests using cajuput tea, derived from its leaves, for treating peptic ulcers. The research was conducted to evaluate the chemical composition of the dried leaves from different stages of leaf age and drying methods. The young, immature, and mature leaves were picked from the tree. Leaves were cleaned and dried in a hot air oven at 40, 45, and 50°C. The tea extracts were then analyzed for acid concentration and phenolic content. The study revealed that drying young leaves using varying acid concentrations significantly affected leaf age; however, the drying methods did not exhibit a notable effect. The highest levels of citric acid, malic acid, and tartaric acid were observed in young leaves. Hot air oven at 40°C resulted in the highest total phenolic content at 137.55mg GAE g-1 DW.
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Effect of BA and IBA on micropropagation of Cannabis sativa L. ‘Hang Kra Rog’
(2024-09-01)
Singhavorachai, P.
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Deewatthanawong, R.
;
Montri, N.
‘Hang Kra Rog’ is an original Thai strain of cannabis (Cannabis sativa L.). Tissue culture methods can rapidly propagate disease-free plants. This study optimized the protocol for surface sterilization and in vitro plant regeneration from explant nodal segments for the propagation of cannabis on an industrial scale. Various Clorox concentrations and soaking periods were used for surface sterilization, with different concentrations of BA and IBA plant growth regulators added to the basal medium for micropropagation of C. sativa L. using nodal explant culture. Results showed that 10% Clorox for 20 min followed by 5% Clorox for 20 min gave the highest explant survival at 75%. Optimal shoot and root development occurred when enriched with 0.5 mg L-1 IBA, resulting in 1.00 shoots, 3.10 roots, 9.97 leaves, and a shoot length of 4.74 cm. The 2 mg L-1 BA yielded the highest number of shoots at 1.87 shoots and resulted in a large callus size of 1.19 cm and callus fresh weight of 0.32 g.
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Transcription factors involved THCAS promoter in cannabinoid biosynthesis pathway of Cannabis sativa L.
(2024-09-01)
Tontiworachai, B.
;
Deewatthanawong, R.
;
Mitsuda, N.
;
Sakamoto, S.
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Chung, K.
Cannabinoids are important secondary metabolites found in Cannabis sativa L. that play a crucial medical role in pain relief and mood adjustment. The main cannabinoid substances in cannabis are delta-9-tetrahydrocannabinolic acid (THCA), and cannabidiolic acid (CBDA). THCA biosynthesis is regulated by the THCA synthase (THCAS) gene which is regulated by the THCAS promoter. However, a comprehensive understanding regarding the regulation of gene expression by transcription factors (TFs) in cannabis is lacking. This study identified the TFs involved in regulating the THCAS promoter in Cannabis sativa L. ‘Hang Kra Rog’ by first amplifying the nucleotide sequences in the promoter of the THCAS gene from cannabis plants using the PCR technique. Subsequently, the THCAS promoter was cloned into the pENTR-5’-TOPO vector for sequencing purposes and then transferred into the R4L1pDEST_HISi vector by the LR reaction for yeast one-hybrid (Y1H) screening to study the interaction between TF proteins from the transcription factor cDNA library and the THCAS promoter within yeast cells. The TF proteins involved in the THCAS promoter were identified into seven groups: ARID, B3, bZIP, CPP, GeBP, HD, and NAC with further verification of their functions in cannabis plant cells using transient effector-reporter analysis.

Montri, Nattaya

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