Montri, Nattaya

Preferred name

Montri, Nattaya

Alternative Name

Montri, N.

Main Affiliation

School of Agricultural Technology

nattaya.mo@kmitl.ac.th

Now showing 1 - 10 of 14

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Effect of ethyl methanesulfonate on growth and morphology of Doritis pulcherrima Lindl.
(2026-01-01)
Supreeworakij, S.
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Saetiew, K.
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Montri, N.
Results showed that 0.5 mg/l BA and 1 mg/l NAA promoted the greatest seedling growth, with increased leaf number, leaf length, plant height, and root development. In the third experiment, protocorm-like bodies were treated with EMS at 0, 0.5, and 1.0% for 60 or 90 minutes. Survival declined with increasing concentration and time, with LD₅₀ values of 0.82% at 60 minutes and 0.54% at 90 minutes. Chlorophyll a, Chlorophyll b, and carotenoids were not significantly affected, but stomatal size changed. Stomatal width and length increased under 1.0% EMS, while density decreased from 220.17 to 149.47 stomata/mm². EMS treatment for 90 minutes showed an increased density relative to 60 min. RAPD analysis using 10 primers revealed high polymorphism indicating EMS induced genetic variation. Cluster analysis divided 22 samples into 9 groups at a similarity coefficient of 0.89, confirming genetic diversity despite no visible morphological changes.
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Preharvest Salicylic Acid Application Enhances Pigments, Antioxidants, and Secondary Metabolites in Cannabis sativa L.
(2026-03-01)
Montri, Nattaya
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Tebdoie, Chachpon
;
Kongchinda, Papitchaya
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Singhavorachai, Pornjarus
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Tontiworachai, Borworn
Salicylic acid (SA) is a key signaling molecule regulating secondary metabolism and stress responses in plants, but its preharvest role as a low-cost elicitor in cannabis remains underexplored. This study evaluated the effects of preharvest foliar SA application at different concentrations and application intervals on pigments, antioxidants, and cannabinoids in Cannabis sativa L. leaves and inflorescences. In leaves, moderate SA (0.1 M) significantly enhanced total phenolic content, total flavonoid content, and antioxidant activity (%DPPH inhibition), while higher concentrations suppressed these responses, reflecting a regulated metabolic trade-off rather than irreversible tissue damage. A significant interaction between SA concentration and preharvest time was observed for chlorophyll a (p < 0.01), whereas chlorophyll b and total chlorophyll were not significantly influenced by the interaction. In inflorescences, short-term application of 0.1 M SA (1 h preharvest) maximized phenolics, flavonoids, antioxidant capacity, and pigment accumulation, whereas the untreated controls showed the lowest levels. Cannabinoids exhibited distinct responses: Δ9-tetrahydrocannabinol (Δ9-THC), total tetrahydrocannabinol (Total THC), and tetrahydrocannabinolic acid (THCA) peaked at 0.1 M SA applied 1 h preharvest, while cannabidiol (CBD) was less concentration-dependent, with maximum accumulation observed at 1.0 M SA applied 24 h preharvest. Preharvest SA elicitation strongly modulated cannabis secondary metabolism. Short-term application of moderate SA promoted total phenolic, total flavonoid, antioxidant, pigment, and THC-group cannabinoid accumulation, while CBD displayed broader tolerance to concentration and application timing. These findings highlighted the potential of SA as a preharvest elicitor to improve cannabis phytochemical quality.
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Effect of calcium phosphate on growth, pigment and secondary compound accumulation in Cannabis sativa L. callus
(2026-05-01)
Tebdoie, C.
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Thipphaaut, T.
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Deewatthanawong, R.
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Kongchinda, P.
;
Singhavorachai, P.
The application of calcium phosphate in the form of tricalcium phosphate (Ca3(PO4)2) on cannabis (Cannabis sativa L.) callus cultures for 7 days significantly increased fresh and dry weights at concentrations ranging from 800 to 2000 mg/L, with no statistical differences between concentrations. However, higher calcium phosphate concentrations led to reductions in pigments and DPPH radical scavenging activity. By contrast, callus cultures treated with 800 and 1600 mg/L showed enhanced total phenolic content and carotenoids, while total flavonoid content increased proportionally. These findings suggested that calcium phosphate concentration influenced the biosynthesis of secondary metabolites in cannabis callus cultures.
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Phytochemical analysis of Melaleuca cajuputi Powell leaves for the development of herbal tea
(2026-06-01)
Montri, N.
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Chalornsiri, S.
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Pato, N.
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Kueasakun, N.
;
Deewatthanawong, R.
The plant Melaleuca cajuputi Powell, commonly known as cajuput, is found in coastal swamp forests in Thailand. Traditional knowledge suggests using cajuput tea, derived from its leaves, for treating peptic ulcers. The research was conducted to evaluate the chemical composition of the dried leaves from different stages of leaf age and drying methods. The young, immature, and mature leaves were picked from the tree. Leaves were cleaned and dried in a hot air oven at 40, 45, and 50°C. The tea extracts were then analyzed for acid concentration and phenolic content. The study revealed that drying young leaves using varying acid concentrations significantly affected leaf age; however, the drying methods did not exhibit a notable effect. The highest levels of citric acid, malic acid, and tartaric acid were observed in young leaves. Hot air oven at 40°C resulted in the highest total phenolic content at 137.55mg GAE g-1 DW.
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Influence of exogenous salicylic acid on phytochemical improvement and antioxidant activity in Cannabis sativa L.
(2024-01-01)
Tebdoie, C.
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Dewatthanawong, R.
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Kongjinda, P.
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Montri, N.
The impact of salicylic acid (SA) foliar spray at varied concentrations (0 and 1 M) and preharvest periods (24, 36, and 42 hours) on secondary metabolite in inflorescences of cannabis (Cannabis sativa L.) was investigated. Result was significantly differed in secondary compound composition. Application of 1 M SA at 24 hours preharvest increased total phenolics, total flavonoids, and total chlorophyll. Moreover, 1 M SA at 24 and 42 hours preharvest showed higher chlorophyll a and b contents than 1 M SA at 36 hours preharvest. While antioxidant activity was not significantly differed among 1 M SA treatments at different preharvest periods, it surpassed non-SA-treated plants. The total pigment was not significantly differed among SA treatments. 1 M SA spray reduced carotenoid content in cannabis inflorescences, with the highest carotenoid observed in non-SA treated plants.
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Ensiling Time and Mixed Microbe Fermented Liquid Modulate In Vitro Digestibility and Rumen Fermentation of Fermented Total Mixed Rations
(2026-01-01)
Polyorach, Sineenart
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Suphalucksana, Wichai
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Klompanya, Ampon
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Yuangklang, Chalermpon
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Wanapat, Metha
This study investigated how varying the ensiling period and the level of mixed microbes fermented liquid (MMFL) influences the chemical composition, in vitro degradability, rumen fermentation profile, and microbial ecology of fermented total mixed rations (FTMR). A completely randomized 4 × 4 factorial design was used, incorporating four fermentation durations (0, 7, 14, and 21 days) and four MMFL inclusion rates (0, 0.5, 1.0, and 1.5% of DM). Both factors exerted significant effects on FTMR quality (p < 0.05). The combination of a 14-day fermentation period with 0.5% MMFL consistently produced the most desirable outcomes. Under these conditions, crude protein concentration rose from 12.0% to 14.3% of DM, while neutral-detergent fiber declined from 54.2% to 49.1%. Improvements were also observed in in vitro digestibility, with DM and OM increasing by 9–12% relative to the untreated control (p < 0.05). Fermentation end-products were enhanced, as total volatile fatty acids increased by 15% (92.4 vs. 80.1 mmol/L), and the molar proportion of propionate increased from 24.5 to 29.2 mol/100 mol, thereby lowering the acetate-to-propionate ratio (2.4 vs. 3.0; p < 0.05). Estimated methane production declined by 18% (p < 0.01). Microbial counts reflected a shift toward a more efficient fermentative community, with bacterial and fungal populations increasing by 21% and 18%, and protozoa decreasing by 25% (p < 0.05). Overall, moderate MMFL supplementation during a 14-day ensiling phase enhanced nutrient conservation and fermentation efficiency, suggesting practical value for improving FTMR utilization in ruminant systems. Further in vivo and economic evaluations remain necessary.
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Effect of BA and IBA on micropropagation of Cannabis sativa L. ‘Hang Kra Rog’
(2024-09-01)
Singhavorachai, P.
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Deewatthanawong, R.
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Montri, N.
‘Hang Kra Rog’ is an original Thai strain of cannabis (Cannabis sativa L.). Tissue culture methods can rapidly propagate disease-free plants. This study optimized the protocol for surface sterilization and in vitro plant regeneration from explant nodal segments for the propagation of cannabis on an industrial scale. Various Clorox concentrations and soaking periods were used for surface sterilization, with different concentrations of BA and IBA plant growth regulators added to the basal medium for micropropagation of C. sativa L. using nodal explant culture. Results showed that 10% Clorox for 20 min followed by 5% Clorox for 20 min gave the highest explant survival at 75%. Optimal shoot and root development occurred when enriched with 0.5 mg L-1 IBA, resulting in 1.00 shoots, 3.10 roots, 9.97 leaves, and a shoot length of 4.74 cm. The 2 mg L-1 BA yielded the highest number of shoots at 1.87 shoots and resulted in a large callus size of 1.19 cm and callus fresh weight of 0.32 g.
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Transcription factors involved THCAS promoter in cannabinoid biosynthesis pathway of Cannabis sativa L.
(2024-09-01)
Tontiworachai, B.
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Deewatthanawong, R.
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Mitsuda, N.
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Sakamoto, S.
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Chung, K.
Cannabinoids are important secondary metabolites found in Cannabis sativa L. that play a crucial medical role in pain relief and mood adjustment. The main cannabinoid substances in cannabis are delta-9-tetrahydrocannabinolic acid (THCA), and cannabidiolic acid (CBDA). THCA biosynthesis is regulated by the THCA synthase (THCAS) gene which is regulated by the THCAS promoter. However, a comprehensive understanding regarding the regulation of gene expression by transcription factors (TFs) in cannabis is lacking. This study identified the TFs involved in regulating the THCAS promoter in Cannabis sativa L. ‘Hang Kra Rog’ by first amplifying the nucleotide sequences in the promoter of the THCAS gene from cannabis plants using the PCR technique. Subsequently, the THCAS promoter was cloned into the pENTR-5’-TOPO vector for sequencing purposes and then transferred into the R4L1pDEST_HISi vector by the LR reaction for yeast one-hybrid (Y1H) screening to study the interaction between TF proteins from the transcription factor cDNA library and the THCAS promoter within yeast cells. The TF proteins involved in the THCAS promoter were identified into seven groups: ARID, B3, bZIP, CPP, GeBP, HD, and NAC with further verification of their functions in cannabis plant cells using transient effector-reporter analysis.
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Exploring Tectona grandis Linn. f. Leaf Extract as a Functional Feed Additive with Antioxidant and Nutraceutical Potential for Livestock
(2025-12-01)
Montri, Nattaya
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Wanapat, Metha
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Kang, Sungchhang
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Cheas, Seangla
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Cherdthong, Anusorn
This study investigated the phytochemical composition, antioxidant activity, and safety of Tectona grandis leaf extracts at four maturity stages. Ethanolic extracts were screened for secondary metabolites and analyzed using GC–MS, together with heavy metal determination, phenolic profiling, and antioxidant assays. Preliminary phytochemical screening revealed the presence of alkaloids, flavonoids, glycosides, tannins, saponins, and coumarins, with clear variation among leaf stages. GC–MS analysis detected multiple bioactive constituents, including phytol, n-decanoic acid, and heptadecenal. These compounds have been previously reported to exhibit antioxidant, antimicrobial, and anti-inflammatory properties. Heavy metal analysis showed very low cadmium concentrations (0.001–0.004 mg/kg), undetectable lead levels (<LOD), and trace levels of arsenic concentrations (0.012–0.018 mg/kg), all of which were within safe limits for plant materials. Quantitative assays demonstrated that mature leaves contained the highest total phenolic (8.751 ± 0.018 mg GAE/g DW), total flavonoid (0.359 ± 0.017 mg QE/g DW), and condensed tannin (0.303 ± 0.000 mg CE/g DW) contents. Correspondingly, mature-leaf extracts exhibited the greatest antioxidant capacity, showing 95.88 ± 0.12% inhibition of oxidant activity. The ethanolic extracts exhibited IC<inf>50</inf> values ranging from 22.93–50.35 mg/mL, whereas the ascorbic acid standard showed an IC<inf>50</inf> of 0.0767 mg/mL, confirming the expected lower potency of crude plant extracts. The strong correlation between phenolic accumulation and antioxidant activity suggests enhanced defense against oxidative stress. The absence of detectable heavy metal contamination, together with the high polyphenolic content, suggests that T. grandis leaves may serve as a safe natural source of antioxidants. These findings demonstrate the strong antioxidant potential of T. grandis leaf extracts, providing a basis for future studies evaluating their functional applications in biological systems.
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Optimizing simplified growing media to enhance cannabis cultivation
(2025-07-01)
Tebdoie, C.
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Khana, A.
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Pajareeyanont, S.
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Deewatthanawong, R.
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Kongchinda, P.
This study evaluated the effects of different growing media on the vegetative growth, chlorophyll content, and inflorescence yield of Cannabis sativa L. ‘Platinum Kush Breath Remix’. A substrate composed of peat moss, perlite, and vermiculite (3:1:1; PM) significantly enhanced plant height, stem diameter, number of nodes, and inflorescence yield. The highest chlorophyll a and total chlorophyll contents were observed in plants grown in a medium comprising coconut coir, perlite, vermiculite, and vermicompost (3:1:1:1; CC1), which was comparable to PM in terms of yield. Both PM and CC1 media significantly outperformed other treatments, indicating their suitability for optimizing C. sativa growth and productivity.

Montri, Nattaya

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