Saetiew, Kanjana

Preferred name

Saetiew, Kanjana

Alternative Name

Saetiew, K.

Main Affiliation

School of Agricultural Technology

kanjana.sa@kmitl.ac.th

Now showing 1 - 10 of 22

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Isolation and expression analysis of the Flavanone 3-Hydroxylase genes in Lotus (Nelumbo nucifera gaertn.), Waterlily (Nymphaea sp.) and transient silencing in Waterlily
(2017-04-01)
Chaipanya, Chaivarakun
;
Saetiew, Kanjana
;
Arunyanart, Sumay
;
Parinthawong, Nonglak
Flavanone 3-hydroxylase (F3H) converts flavanones from dihydroflavonols, which leads to production of flavonoid compounds via the anthocyanin biosynthesis pathway in plants. In this study, the F3H gene was isolated from lotus (Nelumbo nucifera Gaertn.) cv. Buntharik (white petal lotus), cv. Satabankacha (pink petal lotus), and waterlily (Nymphaea sp.) var. St. Louis Gold by reverse transcription PCR (RT-PCR). The open reading frames (ORF) of three cultivars’ genes were 1,134 bp in length, encoding a predicted protein of 377 amino acids. Their nucleotide sequences were identical, and the amino acid sequence shared high homology to F3H from different plant species. Expression of F3H was specifically regulated in petals and stamens, while less expression was found in leaf tissue of waterlily variety St. Louis Gold. The correlation of F3H expression according to specific colouration was performed in waterlily. The F3H gene was more highly expressed in decreasing order of red, purplish blue, and yellow petals when compared using semi quantitative PCR (sqPCR). Gene regulation according to flowering stage and pigmentation was determined in lotus. The F3H expression was slightly diminished in petals of cultivar Satabankacha at the fully-opening stage, whereas it was detected in the cultivar Buntharik only when white petals were tinted with pink. An RNAi gene-silencing vector, pJA8F3H, encoding a hairpin F3H RNA, was introduced to waterlily petals using the Agrobacterium infiltration method, and the F3H expression was analysed at 1 and 3 days post infiltration (dpi) by sqPCR. The results showed that the F3H expression was down-regulated at 3 dpi in flowers tested of the red petal variety and purplish blue petal variety compared to controls. The results confirmed that pJA8F3H is efficient and could be used as a transformation vector to transiently suppress F3H expression in waterlily or lotus.
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Transformation of antisense dihydroflavonal 4-reductase (DFR) into sacred lotus 'Buntharik' using Agrobacterium-mediated gene transfer
(2014-03-13)
Saetiew, K.
;
Leethaweesup, W.
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Parinthawong, N.
;
Arunyanart, S.
Transformation of antisense dihydroflavonal 4-reductase (DFR) into 'Buntharik' sacred lotus (Nelumbo nucifera Gaertn.) using Agrobacterium-mediated gene transfer was experimented. The A. tumefaciens strain EHA105 harbored two binary vectors. The pCAMBIA2301anti-DFR plasmid contained neomycin phosphotransferase (NPTII) as a selectable marker gene, the antisense DFR as an inserted gene and the β-glucuronidas gene (GUS) as a reportor gene. The pBI121anti-DFR plasmid contained NPTII gene and the antisense DFR. Twomonths-old calli regenerated from apical buds were used as plant materials. The plant materials were soaked in the Agrobacterium suspension for 10 or 30 min. The calli were co-cultivated for two days in the darkness. Treated calli were selected on solid MS medium containing 50 mg L-1 kanamycin and 250 mg L-1 cefotaxime. The calli were transferred to the same medium every two weeks for eight weeks. The survived calli regenerated shoots on MS medium containing a combination of 0.54 μM NAA and 4.44 μM BA. Both plasmids gave the best callus growth, maximum callus size and the survival percentage when soaked for 10 min. GUS bioassay was used to verify the presence of GUS gene in petioles and leaves of transgenic plants. It was found that petioles and leaves of transformants which were transformed with pCAMBIA2301anti-DFR by soaking for 30 min showed the highest percentage of blue spots on explants. Of all 14 PCR positive clones, 5 clones showed GUS gene, 11 clones contained NPTII gene and 6 clones had DFR gene.
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Transformation RNA interference-mediated silencing of the Flavanone 3-Hydroxylase gene in Lotus (Nelumbo nucifera Geartn. cv. Buntharik)
(2020-07-01)
Saetiew, K.
;
Konee, C.
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Parinthawong, N.
;
Arunyanart, S.
RNA interference mediated-silencing of the F3H gene in the lotus (Nelumbo nucifera Geartn.) cv. Buntharik inhibited the anthocyanin pathway after gene transformation. The plasmid pJA8F3H contained the inverted repeats of F3H fragments, bar gene as a selectable marker in plants, which is resistant to basta (glufosinate amonium). The transformation of particle bombardment was obtained with multiple shoots of lotus. F3H gene expression analysis showed that expression of F3H decreased in transgenic lotus when compared with the control. On the first and the second test, 5 transgenic lotus of the relative expression F3H gene was detected with 0.0496 and 0.466 times, respectively. The inhibition of gene expression decreased when they were cultured for a long time. The expression F3H gene was detected by RT-PCR, and real-time PCR resulted in consistent decreasing in the transgenic lotus compared with the non-transgenic lotus, which was able to inhibit the expression F3H gene.
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Effect of paclobutrazol on growth retarding in potted chrysanthemum (Dendranthema grandiflora)
(2024-03-01)
Piphatwatthanakul, P.
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Boonkamjat, T.
;
Saetiew, K.
Paclobutrazol application was compared to the control. It showed a positive influence in reducing stem height, canopy width, internode length, node number, and flower size. Besides, methods of application were insignificantly found. As for the second experiment, decreased concentrations to 0, 100, 200, 300, and 400 ppm of paclobutrazol were tested, using the same manner of application as in the first experiment. It appeared that all concentrations of paclobutrazol decreased canopy width, stem length, internode length, and node number when compared with the control treatment, similar to results of the experiment 1. Nevertheless, it occurred in Prakaimat variety that both foliar spraying and soil drenching of paclobutrazol led to increasing chlorophyll content and carotenoid level. Meanwhile, Fiona chrysanthemum responded better to foliar spraying method than of soil drenching.
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Influence of plant growth regulators on shoot development of Chrysanthemums
(2024-01-01)
Boonkamjat, T.
;
Saetiew, K.
;
Teerarak, M.
Plant nodes were cultured on MS mediums supplemented with multiple concentrations of plant growth regulators. Sunny Snow produced the largest callus of 1.19×1.19 cm and obtained 93.33% of new shoots with 10 . 0 0 shoots per callus, and 0. 84 g callus fresh weight, when grew in MS medium supplemented with 1 mg/l BA + 0.1 mg/l IAA as compared to the control which no callus was produced. After 20 weeks of culturing, the second set showed that the control treatment of Sunny Snow gave the highest stem growth and the best canopy size of 9.00 and 5.60 cm respectively. Chompoo Phan gave the highest scores of chlorophyll content, i.e., chl a 652.14 µg/g FW, chl b 332.47 µg/g FW and carotenoid 165.92 µg/g FW. Besides, MDA appeared to be highest, 17.37 nmol/g FW when cultured on MS medium supplemented with 1 mg/l BA + 0.1 mg/l IAA.
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Callus and hairy root induction of Melaleuca cajuputi Powell
(2020-05-01)
Prangthong, U.
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Montri, N.
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Saetiew, K.
It is stated that the lowest percentage of contamination at 49.63% and highest percentage of survival at 37.95% after sterilized with 70% ethanol for 1 min and 1.8 % NaOCl for 30 min. The bud explants were cultured on Murashige and Skoog (MS) medium supplemented with 0, 0.02, 0.04, 0.06, 0.08 mg/l 2,4-Dinitrophenylhylhydrazine (2,4-D) combination with 0, 0.02, 0.04, 0.06, and 0.08 mg/l Thidiazuron (TDZ) for callus induction. The results showed that the explants were cultured on MS medium supplemented with 0.06 mg/l TDZ resulting to be suitable for callus induction with biggest size at 65.2x1. 22cm of width and length. Culturing buds on MS medium supplemented with 0.02 mg/l TDZ had the highest averaged shoot number. The roots were cultured in liquid MS medium supplemented with 0.04 mg/l TDZ, 0.04 mg/l 2.4-D gave the highest weight of hairy roots.
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Improvement of Dendranthemum grandiflora cv. canter with colchicine in vitro
(2020-01-01)
Asoko, N.
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Ruamrungsri, S.
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Yoosumran, V.
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Saetiew, K.
The mutation of chrysanthemum with ray florets was induced by colchicine. The explants were cultured on Murashige and Skoog (MS) medium supplemented with 2 mg/l NAA and 4 mg/l Kinetin for inducing calli for 12 weeks. The shoots were regenerated from callus when cultured on MS medium for 8 weeks. The plant growth, size of stomata and number of chloroplasts per stomata were not statistically different in each treatment. However, the ray florets were received high levels of colchicine is less to survivor rate. The plantlet of chrysanthemum was transfered to pot plant containing soil and chopped coconut. Five of ten plant showed the flowers color changed from purple into a pinkish-orange at the 0.10% colchicine for 12 hours. The explant obtained from soaked of 0.15 % colchicine for 12 hours with the highest number of flowers per plant, 72 flowers per plant.
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Peppermint essential oil extends the vase life of the cut Dendrobium orchid ‘White Sanan’
(2023-01-01)
Teerarak, M.
;
Saetiew, K.
;
Laosinwattana, C.
The vase life of cut orchid flowers is significantly extended by preservative solutions containing silver compounds. However, these compounds are toxic and require careful handling and disposal. As a result, the demand is increasing for natural preservation materials such as plant essential oils as they are biodegradable, ecofriendly, and safe alternatives for enhancing vase life and postharvest cut flower quality. The objective of this investigation was to study the effects of an emulsion of glucose and peppermint essential oil on vase life and postharvest quality of the popular cut Dendrobium orchid ‘White Sanan’. The orchids were preserved in vase solutions of distilled water or 4% glucose + 0.005-0.02% peppermint essential oil. The essential oil treatment increased the vase life of the flowers and the inflorescences by more than 6 days longer than the control treatment. The combination of glucose and peppermint essential oil increased water uptake and the fresh weight of cut orchids and delayed wilting time and abscission of floral buds and open florets. Moreover, treatment with the vase solution containing 4% glucose + 0.005% peppermint essential oil resulted in the highest bud opening rate (86.9%). Our results suggest that applying 4% glucose and 0.005-0.02% peppermint essential oil is especially effective as a preservative solution for the Dendrobium orchid ‘White Sanan’.
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Effects of organic compound and plant growth regulators on seed germination and growth of Doritis pulcherrima Lindl. in vitro
(2024-09-01)
Supreeworakij, S.
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Saetiew, K.
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Rungkitwattananukul, T.
;
Phanchompoo, P.
;
Montri, N.
Doritis pulcherrima Lindl. is one of the wild orchids of which the distribution has decreased in nature and endangered. In vitro seed culture by using plant tissue culture is a proper method to help increasing the numbers. The purpose of this research was to study the effect of organic compound and plant growth regulators on seed germination and growth of D. pulcherrima in vitro. Seeds were cultured on Vacin and Went (VW) basal medium. Experiments were performed in a 5×7 factorial in completely randomized design (factorial in CRD) 2 factors; Factor 1 capsule of D. pulcherrima with 5 treatments such as Pi102/1, Pi102/2, Pi119/1, Pi119/2 and Pu112. Factor 2 organic compound with 7 treatments such as no organic compound (control), 50, 100 g L-1 raw namwa banana powder, 50, 100 g L-1 cavendish banana homogenate, 50, 100 g L-1 potato homogenate. After 13 weeks of culture, there were only 3 capsules growth seed germination (Pi119/2, Pi102/2 and Pu112/1) and the highest percentage of seed germination was Pi119/2 on VW medium supplemented with 50, 100 g L-1 banana powder and 50 g L-1 potato homogenate gave the highest germination percentage at level 4 (75-100%). From the results, organic compound is a suitable culture supplement for D. pulcherrima seed germination and development compared to no organic compound. In addition, the effect of plant growth regulators on growth and development of D. pulcherrima protocorm were cultured on VW medium supplemented with different concentrations of control, 1, 2 and 3 mg L-1 BA and 0.01, 0.02 and 0.03 mg L-1TDZ. The result showed the control gave the highest number of leaves at 6.50, the number of protocorm at 3.67 and fresh weight of protocorm at 0.42 g. The protocorm and plantlet were induced after 10 of culture.
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Induction of calluses from petal of three chrysanthemum cultivars in vitro
(2024-09-01)
Boonprasop, S.
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Saetiew, K.
;
Teeralak, M.
Propagation and breeding of chrysanthemum can be done via petal tissue culture technique. Since it has been well known that plant growth regulator has a rather wide range of physiological effects on diverse plant genetic resources, such effects were thus determined in petal induced callus of three chrysanthemum cultivars, i.e., ‘Pingpong Golden’, ‘Scarlet’ and ‘Karaked’. Chrysanthemum petals were detached from the plants of the three cultivars as mentioned then cultured on MS medium containing different formulas of plant growth regulators, i.e., 2 mg L-1 BA + 0.5 mg L-1 NAA; 2 mg L-1 BA + 1 mg L-1 2,4-D; 1 mg L-1 BA + 1 mg L-1 2,4-D + 1 mg L-1 Kinetin and 2 mg L-1 NAA + 4 mg L-1 Kinetin, as treatment 1-4, respectively. The cultures were put under white LED light (PPFD 40 µmol m-2 s-1) 16 h day-1 at 25±3°C for 12 weeks. The results showed that ‘Karaked’ exhibited the shortest callus induction period of 6-12 days and the highest fresh weight of callus growth (2.03-0.05 g) when cultured on MS medium supplemented with 1 mg L-1 BA + 1 mg L-1 2,4-D + 1 mg L-1 Kinetin. The callus ‘Scarlet’ performed highest chlorophyll content (chl a 98.59 mg g-1 FW, chl b 44.72 mg g-1 FW and carotenoid 24.26 mg g-1 FW) whereas compact callus with highest number of 4 shoots explant-1 were obtained in ‘Scarlet’ cultured on MS medium supplemented with 2 mg L-1 NAA + 4 mg L-1 Kinetin.

Saetiew, Kanjana

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