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A new method to increase plant quantity through tissue culture on Vitex negundo L
(2022-09-01)
Poeaim, A.
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Prasertkittikul, N.
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Wangsor, N.
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Boonmee, W.
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Laipasu, P.
A new method to increase plant quantity through tissue culture on Vitex negundo L. was discovered. The influences of PGRs on shoot regeneration cultured on MS medium with 0.5, 1, 2, or 3 mg/L benzylaminopurine (BAP), meta-Topolin (mT) and Thidiazuron (TDZ) were resulted to be the greatest percentage of shoot development was 100% at 1 mg/L BAP in MS medium. The greatest average number of shoot multiplication was 4.40 shoots at 0.5 mg/L BAP in MS medium after 8 weeks of culture. For roots, the result of MS media fortified with 0.5, 1, 2, and 3 mg/L of indole 3 butyric acid (IBA) and naphthalene acetic acid (NAA) discovered that the excellent percentage of root production was 90% at 3 mg/L IBA. The highest average number of root production was 108.20 roots on 2 mg/L NAA after 8 weeks of culture. The plantlets were eventually transferred into the plastic pots. In this research work, the nodes were demonstrated to be an outstanding regenerating explant resource for Vitex negundo L. An effective regeneration strategy for plantlets derived from the nodes was demonstrated.
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Effects of 6 benzylaminopurine and meta topoline on micropropagation of Dendrobium chrysanthum Wall. ex. Lindl
(2022-09-01)
Poeaim, A.
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Kunwanlop, W.
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Boonmee, W.
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Laipasu, P.
The results of solid Gamborg's B5 medium at 2 mg/L mT found the highest average number of shoots at 1.6 shoots per explant; and the number of leaves at 3.7 leaves per explant. The concentration of 1.5 mg/L of mT showed the highest average length of shoots which was 9.82 mm, while the 0.5 mg/L mT revealed the most significant number of roots which were 0.9 roots per explant with the longest root of 3.75 mm. The liquid Gamborg's B5 medium showed the highest average number of shoots at 1.5 mg/L BAP, the same as at 1.5 mg/L mT. The medium without plant growth regulator and 1 mg/L mT was the highest average length of shoots of 13.98 mm. The highest number of roots per explant was 0.8, similar to 2 mg/L mT, but the different maximum length of roots of 3.04 mm. A hundred percent of protocorms induced shoots found in all media. The plantlets were survived after transplanting into pots and covered with plastic bags in four weeks.
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The effects of abscisic acid and sorbitol on the slow growth in vitro preservation of Mahesak (Tectona grandis L.)
(2020-09-01)
Tongsad, P.
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Laipasu, P.
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Chareonsap, P. P.
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Poeaim, Anurag
The conservation of teak (Tectona grandis L.) by slow growth was proceeded out to determine appropriate methods in vitro preservation using osmotically active compounds and growth retardants. Calli and node explants were cultured on Murashige and Skoog (MS) and Woody Plant Medium (WPM) media supplemented with 1, 2 and 3 mg. L-1abscisic acid (ABA) containing 30 g.L-1sucrose and hormone-free MS and WPM media containing 0.1, 0.2 and 0.3 M sorbitol for reduced the growth rate. Result showed that preservation of calli on MS and WPM media supplemented with 0.2 and 0.3 M sorbitol and node explants were cultured on WPM medium supplemented with 0.2 and 0.3 M sorbitol inhibited the growth and maintained the quality of calli and node explants for 12 weeks. It was found that ABA could not inhibit the growth rate after cultures for 12 weeks. Callus and node explant survival from slow growth preservation were able to regrowth which successfully (100%) tested on WPM medium supplemented with 0.25 mg.L-16-benzylaminopurine (BAP) containing 30 g.L-1sucrose after for 2 weeks.
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The effect of plant growth regulator and in vitro conservation of teak (Tectona grandis L.) by tissue culture
(2018-12-01)
Tongsad, P.
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Laipasu, P.
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Chareonsap, P. P.
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Poeaim, A.
The plant regeneration of teak (Tectona grandis L.) was reported. Result showed that the highest number of shoot induction was 55.49 cm., in length of shoots which tested at 0.25 mg·L -1 BAP, and averaged 40.29 cm which tested at 0.25 mg·L -1 mT after 4 weeks. The shoots were elongated using shoot tip explants of cryopreservation on teak by encapsulation-dehydration (EnDe), encapsulation-vitrification (EnVi) and vitrification (Vi) techniques. The shoot tips were used as explants, encapsulated apices were precultured on hormone-free MS medium containing 0.3 M sucrose for 2 days, dehydrated, and immersed in liquid nitrogen for 24 h after that thawed in thermal bath at 40 ºC and washed in unloading solution. Vi and EnVi protocols were used to treat with a loading solution, dehydrated by plant vitrification solution 2 (PVS2). EnDe protocol were encapsulated by desiccated air-dry in laminar air flow and dehydrated with silica gel. Results showed that Vi gave an excellent shoot tip explants for survival and regrowth. Vi were unable to survival. This experiment was preliminary searched to improve and develop the teak for cryopreservation.
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Effect of plant growth regulators on micropropagation of Vanilla aphylla and Vanilla planifolia sp. Variegate
(2018-12-01)
Kunwanlop, W.
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Boonmee, W.
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Laipasu, P.
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Chareonsap, P. P.
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Krajangvuth, T.
The plant growth regulators for propagating large number of vanillas in a short period of time was reported. Axillary buds were used from the explants of Vanilla aphylla and Vanilla planifolia sp. variegata that cultured on solid Gamborg's B5 medium supplemented with 0, 0.5, 1, 1.5 and 2 mg/L 6-benzylaminopurine (BA) for 8 weeks. The result showed that the highest average number of shoot proliferation and root induction were obtained at 1 mg/L BA of 3.2 cm. and 5.3 cm. respectively for Vanilla aphylla. Vanilla planifolia sp. variegata showed the highest shoot elongation of 1.9 cm. at 0.5 mg/L BA. Root induction was the highest of 0.9 cm. at 2 mg/L BA. After 12 weeks of culture The explants were grew the best after 12 weeks. They were transplanted into pots and covered by plastic bags for acclimatization and has maintained in greenhouse.
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KMITL organic agriculture model: A review article
(2018-12-01)
Soytong, K.
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Poeaim, S.
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Pongnak, W.
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Luenam, L.
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Poeaim, A.
KMITL organic agriculture model is established by supporting from King Mongkut's Institute of Technology Ladkrabang (KMITL), Bangkok, Thailand to contribute the research findings of biological products as agricultural inputs to be used for organic agricultutre in practice. It is located at Dan Chang district, Suphanburi province, Thailand. It is to promote the farmers to gain the based knowledge of organic agriculture. KMITL organic model are divided into six parts as follows:- production, agricultural inputs, organic certification, marketing, extension and training as well as research and development. The model is a processes from production to marketing including research findings which necessary to serve the model.
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Induction of shoot and root from nodes of Kadsura heteroclita
(2018-12-01)
Jedoroh, N.
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Laipasu, P.
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Chareonsap, P. P.
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Poeaim, A.
Results described on shoot and root induction from node of medicinal plant Kadsura heteroclita. The sterilized nodes were used as explants and cultured on solid synthetic medium, Murashige and Skoog (MS) medium supplemented with single or combined Plant Growth Regulators (PGRs) of 6-benzylaminopurine (BAP), meta-Topolin (mT), Indole-3-butyric acid (IBA),1-Naphthaleneacetic acid (NAA) and Gibberellin (GA <inf>3</inf> ) were applied. The best result for shoot induction was shown on the medium supplemented with 0.5 mg/L of mT after 8 weeks. Transferred shoot to MS medium supplemented with combination PGRs of 0.5 mg/L of mT and 0.5, 1.0, 1.5 and 2.0 mg/L of IBA NAA or GA <inf>3</inf> after 16 weeks revealed that the explant were developed to multiple shoots which averaged number of shoot were 3 shoots/explant. The roots were not perfectly exhibited like sore on medium supplemented with 0.5 mg/L of mT and 1.5 mg/L of GA <inf>3</inf> . The high concentration of Auxin inhibited growth roots. The root induction was done by cutting the shoot and cultured on MS medium supplemented with combination PGRs of 0.5 mg/L of mT and 0.01, 0.02, 0.03, 0.04 and 0.05 mg/L of IBA after 4 weeks found that root length was approximately 2 mm on 0.5 mg/L of mT and 0.05 mg/L of IBA.
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Callus induction and cell suspension culture from leaves of kadsura coccinea
(2018-11-01)
Jedoroh, N.
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Poeaim, A.
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Laipasu, P.
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Chareonsap, P. P.
Kadsura spp. is a vine glabrous woody which belongs to Schisandraceae that is rare plant found in highland. It is an ancient plant group that low adaptation for living. Shape and scent of fruits like sugar apple, and edible when ripping. It contains the nutrition value and high antioxidants. It is a valuable medicine to prevent the tumor HIV resistant and hepatitis. In presently Kadsura spp. become extinct. The calli from leaves of Kadsura spp. was induced by this study. The leaves were sterilized and cultured on solid synthetic medium, Murashige and Skoog (MS) medium supplement with Plant Growth Regulators (PGRs) were used as 0.5, 1, 2, 3 and 5 mg/L concentrations of 6-benzylaminopurine (BAP), meta-Topolin (mT), 2, 4-dichlorophenol-xyacetic acid (2,4-D) and 0.5 mg/L of BAP combined with 0.5, 1, 2, 3 and 5 mg/L of 2,4-D. The maximum number of leaves were induced calli of 55.55 % and 350.16 mm3 averaged area on medium with 0.5 mg/L of BAP with 0.5 mg/L of 2,4-D, but 0.5 mg/L of BAP with 2 mg/L of 2,4-D gave the highest average area (1,079.53 mm3) after 4 weeks. The growth rate of cell suspension cultured in liquid MS medium supplement with 0.5 mg/l of BAP and 0.5 mg/l of 2,4-D resulted the fresh weight and dry weight of cell suspension with the best grown for 15 days of 1.12 g/10 mL, 0.14 g/10 mL fresh and dry weight respectively. Cell suspension was rapidly grown during the period of 6-15 days. The cell suspension was cultured on MS solid medium combine with 0.5 mg/l of BAP and 0.5 mg/l of 2,4-D and 0.2% (W/V) AC after 4 weeks. The best result for calli induction and perfectly for plant regeneration were the MS medium without AC. This research work is developed an optimized protocol for plant breeding.
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Isolation and identification of phytophthora sp. and pythium sp. from durian orchard in Chumphon Province, Thailand
(2018-05-01)
Suksiri, S.
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Laipasu, P.
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Soytong, K.
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Poeaim, S.
The major problem of durian cultivation are root and stem rot disease caused by Phytophthora and Pythium species. Therefore, the pathogens were isolated, identified, and found the virulent pathogenic fungi that isolated from soil planted to durian in Chumphon province. The pathogens were studied on morphological and molecular analysis based on internal transcribed spacer (ITS)-nrDNA sequence. Ten isolates were confirmed as Phytophthora palmivora, Pythium cucurbitacearum, Pythium deliense, Pythium splendens, Mortierella chlamydospora, Mortierella capitata and Mortierella sp. The pathogenicity test was done by detached leaf method in Monthong durian variety. It found that P. palmivora and P. cucurbitacearum were high pathogenic isolates. Moreover, P. splendens was infected and produced symptoms on leaves. It is the first report of P. splendens as pathogenic to durian.

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