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    Item type:Publication,
    Effects of thymol–carvacrol combined with organic acids on carcass traits, meat quality, and stress indicators in broilers under enteric disease challenge
    (2026-01-01)
    Busayakanit, P.
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    Philatha, A.
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    Rassmidatta, K.
    ;
    Ruangpanit, Y.
    The effects of a feeding program combining thymol–carvacrol and organic acids were evaluated as an alternative to an antibiotic growth promoter (AGP) in broilers under a coccidial challenge. A total of 750 male Ross 308 chicks were assigned to a negative control (T1), an AGP group with bacitracin methylene disalicylate (T2), or a thymol-carvacrol–organic acid group (T3). Slaughter weight, dressing percentage, retail cut yields, and meat quality traits did not differ among treatments (P > 0.05). T2 had the highest number of birds without foot pad lesions, significantly exceeding T1 and comparable to T3, which also outperformed T1 (P < 0.05). T2 recorded the fewest mild and severe lesions, while T3 showed a numerical reduction in severe lesions compared with T1. Both T2 and T3 reduced lesion severity relative to T1, with T2 showing the most favorable scores and T3 demonstrating meaningful potential as a non-antibiotic alternative. Thigh meat malondialdehyde (MDA) levels increased during storage (P < 0.01), but no treatment effects were observed (P > 0.05). Serum MDA and the proportions of lymphocytes, monocytes, eosinophils, and basophils were unaffected; however, heterophil percentage and the heterophil-to-lymphocyte ratio were significantly higher in T1 than in T2 and T3 (P < 0.01), indicating reduced physiological stress with both AGP and thymol-carvacrol–organic acid supplementation. Overall, thymol–carvacrol with organic acids-maintained carcass yield and meat quality while mitigating stress responses and improving foot pad health, supporting its potential as a viable alternative to AGPs in broilers under enteric challenge.
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    Item type:Publication,
    Fructooligosaccharides supplementation: effects on broiler chicken performance, intestinal morphology, microbial community, and stress indicators
    (2025-03-01)
    Phungkeha, P.
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    Tiyaprasertkul, P.
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    ; ;
    Ruangpanit, Y.
    Feeding broilers with different amounts of fructooligosaccharides (FOS) under conditions of higher stocking densities and reused litter did not affect growth performance (p>0.05). However, FOS supplementation improved gut health by increasing villus height to crypt depth ratio. It also promoted the growth of Lactobacillus and reduced the incidence of Escherichia coli. The decrease in heterophils to lymphocytes ratio in FOS-supplemented group indicates a reduction in stress levels.
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    Item type:Publication,
    Thymol-carvacrol supplementation in broilers: impact on performance, blood biomarkers, and gut health
    (2025-03-01)
    Tiyaprasertkul, P.
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    Phungkeha, P.
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    ;
    Philatha, A.
    ;
    Rassmidatta, K.
    The stress conditions are induced by a high-dose live coccidiosis vaccine, and thymol-carvacrol supplementation in broilers were significantly improved the feed conversion ratio (FCR) during the starter and grower phases as compared to the negative control. In contrast, antibiotic supplementation resulted in better growth performance throughout the rearing period. In addition, antibiotic supplementation lowered serum levels of the oxidative stress marker malondialdehyde (MDA) and the pro-inflammatory cytokine interleukin-17 (IL-17), with thymol-carvacrol supplementation showing comparable but less pronounced effects, followed by the negative control. In addition, antibiotic supplementation improved intestinal morphology as evidenced by the decreased crypt depth and increased villus height to crypt depth ratio, followed by thymol-carvacrol supplementation compared to the non-supplemented groups. These results suggest that thymol-carvacrol supplementation can improve the gut health and performance of broilers under stress conditions.