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Item type:Publication, Co-stimulation with piezoelectric PVDF films and low intensity pulsed ultrasound enhances osteogenic differentiation(2025-08-01); Aguilar-Tadeo, JulioBone tissue engineering has emerged as a promising approach to address the challenges of bone fracture repair and regeneration. The application of external stimuli (mechanical and electrical) can drive specific cellular responses and osteogenic differentiation, leading to the development of more effective treatments. Piezoelectric materials modulate cellular proliferation and osteogenic differentiation under both static (without mechanical stimulation) and dynamic (with mechanical stimulation) conditions, activating distinct gene expression pathways. In this work, we investigate the combinatorial effect of poly (vinylidene fluoride) (PVDF) poled and non-poled films, and low-intensity pulsed ultrasound (LIPUS) on early-stage osteogenic differentiation of mouse pre-osteoblasts. Static culture with PVDF poled films enhanced Runx2 and Col1α1 expression without impacting alkaline phosphatase (ALP) activity. Inhibition of ERK phosphorylation using U0126 in PVDF poled films resulted in a ~ 6–8-fold increase in ALP activity, suggesting the involvement of an alternative pathway in osteogenic differentiation. Dynamic culture with LIPUS generated an electric potential of approximately 500 mV across PVDF films and an electrical field of 0–10 mV mm<sup>−1</sup>. Co-stimulation led to a ~3-fold increase of ALP activity on stimulated PVDF compared to unstimulated films. This study underscores the potential of piezoelectric materials as non-invasive electrical stimulators to enhance the efficacy of ultrasound-based therapies for bone fracture repair.1 - Some of the metrics are blocked by yourconsent settings
Item type:Publication, Effects of dietary superworm (Zophobas morio) oil on growth and reproductive performance of female zebrafish (Danio rerio)(2023-05-01) - Some of the metrics are blocked by yourconsent settings
Item type:Publication, Validation of reference genes for the normalization of RT-qPCR gene expression in Bacillus siamensis 1021 grown in different culture media(2022-01-01)1 - Some of the metrics are blocked by yourconsent settings
Item type:Publication, Cellular expression profiles of Epstein-Barr virus-transformed B-lymphoblastoid cell lines(2020-01-01); Buranapraditkun, SupraneeEpstein-Barr virus (EBV) can infect human B cells and is associated with various types of B cell lymphomas. Studies on the global alterations of the cellular pathways mediated by EBV-induced B cell transformation are limited. In the present study, microarray analysis was performed following generation of two EBV-infected B-lymphoblastoid cell lines (BLCL), in which normal B cells obtained from two healthy Thai individuals and transcriptomic profiles were compared with their respective normal B cells. The two EBV-transformed BLCL datasets exhibited a high degree of similarity between their RNA expression profiles, whereas the two normal B-cell datasets did not exhibit the same degree of similarity in their RNA expression profiles. Differential gene expression analysis was performed, and the results showed that EBV infection was able to dysregulate several cellular pathways in the human B-cell genes involved in cancer and cell activation, such as the MAPK, WNT and PI3K-Akt signaling pathways, which were upregulated in the BLCL and were associated with increased cellular proliferation and immortalization of EBV-infected B cells. Expression of proteins located in the plasma membrane, which initiate a biological response to ligand binding, were also notably upregulated. Expression of genes involved in cell cycle control, the p53 signaling pathway and cellular senescence were downregulated. In conclusion, genes that were markedly upregulated by EBV included those involved in the acquisition of a tumorigenic phenotype of BLCL, which was positively correlated with several hallmarks of cancer.2 - Some of the metrics are blocked by yourconsent settings
Item type:Publication, Ability of marine cyanobacterium Synechococcus sp. VDW to remove ammonium from brackish aquaculture wastewater(2019-02-01); Karnchanatat, AphichartNitrogen-rich wastewater is a major problem for the aquaculture industry. To investigate whether algae could be used to remove ammonium from brackish shrimp aquaculture wastewater, marine cyanobacterium Synechococcus sp. VDW was cultured in BG11 medium supplemented with Turks Island salt solution and different concentrations of NH<inf>4</inf>Cl (1.0–40.0 mg L<sup>−1</sup>) for 18 days. The cell density of the Synechococcus sp. VDW cultures increased in medium containing between 1 and 10 mg L<sup>−1</sup> of NH<inf>4</inf>Cl, while ammonium concentrations greater than 20.0 mg L<sup>−1</sup> had a negative effect on growth. Glutamine synthetase and glutamate synthase activities were also examined, and were found to increase with cell density. Meanwhile, glutamate dehydrogenase activity increased in response to high NH<inf>4</inf>Cl concentrations (20.0–40.0 mg L<sup>−1</sup>). The cellular response to ammonium excess was confirmed by measuring gene expression levels using quantitative PCR. Expression of both glnA and gltB was down-regulated compared with the control, while that of gdhA was up-regulated. At an initial concentration of 1–10 mg L<sup>−1</sup>, 98–100% of the ammonium was removed by day 6 of cultivation. Therefore, these findings suggest that Synechococcus sp. VDW can remove ammonium from contaminated brackish water, and may be helpful for improving the quality of aquaculture wastewater.