Kramchote, Somsak
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Preferred name
Kramchote, Somsak
Alternative Name
Kramchote, S.
Main Affiliation
Email
somsak.kr@kmitl.ac.th
14 results
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Item type:Publication, Gene actions of resistance to anthracnose (Colletotrichum acutatum) of six F1 hybrid chili cultivars at the mature green fruit stage(2023-01-01) ;Sangarun, P.; ; ;Tsai, W. S.Techawongstien, S.Anthracnose is an economically important fungal disease affecting the yield and fruit quality of chili in the tropics. This study aimed to assess the anthracnose disease reaction in six hybrid chili pepper cultivars compared with their parental lines. Two male resistant lines were crossed with three commercial female parents by North Carolina mating design II. All chili genotypes were grown in the greenhouse in a randomized complete block design with three replications, 10 plants each. Five mature green fruit plant<sup>-1</sup> were harvested and inoculated with C. acutatum by using the microinjection method. ANT4 had smaller lesions than ANT10. Additionally, the F1-hybrids derived from ANT4 showed smaller disease lesions (7.47-9.82 mm) than on those derived from ANT10 (9.40-11.04 mm). However, the percentage heterosis of anthracnose disease traits in ‘Jindanil 80’ (JD) and ANT4 gave the lowest value to other parents. Hybrid JD × ANT4 showed the lowest anthracnose lesion size. However, the heterosis of cross the NM × ANT10 was the most negative at -43.68. The mode of gene action was found to be additive rather than involving dominant effects, with a narrow-sense heritability of 50.9% and broad sense heritability of 70.1%. Therefore, the resistant parent ANT4 cultivar is crucial for use as a resistant elite line for producing good F1 hybrid tolerance to anthracnose disease. - Some of the metrics are blocked by yourconsent settings
Item type:Publication, Effects of Aloe vera Gel Coating on Quality and Shelf Life of Lime (Citrus aurantifolia) Fruit During Ambient Storage(2022-01-01) ;Pimsorn, Orawan; Lime (Citrus aurantiifolia var. Pan) fruits were coated with Aloe vera gel (AVG) at 0 (distilled water as control), 10, 20, 30, 40, and 50% concentration as a 5 min dip and stored at ambient temperature (29 ± 1°C, 74 ± 5% RH) for 16 days. AVG coating markedly slowed fruit yellowing based on color scores and CIE L*, a*, and b* values, resulting in an increase in shelf life of about four days longer than that of the control (10 days). It did not significantly affect other fruit responses; AVG-coated and uncoated fruit had comparable weight loss, juice content, total soluble solids (TSS), titratable acidity (TA), and vitamin C content. Thus, AVG coating improved shelf life of lime primarily through delayed yellowing without adverse effects on other physicochemical attributes. - Some of the metrics are blocked by yourconsent settings
Item type:Publication, Combining ability analysis in anthracnose-resistant introgression inbreds and developing improved resistant lines of pepper (Capsicum annuum L.)(2025-01-01) ;Saengarun, Patcharapan; ;Inprasert, Hathaipat ;Kumsee, NattikaTsai, Wen ShiChili peppers (C. annuum L.) are a commercial spice crop in the tropics. In Thailand, chili pepper is a cash crop for farmers and has cultural significance in Thai cuisine. Anthracnose disease caused by Colletotrichum spp. is responsible for a significant reduction in the marketable fruit yield of chili peppers. In this study, we tested the combining abilities of anthracnose-resistant chili pepper lines possessing resistance introgression genome segments from C. chinense (PBC932) and C. baccatum (PBC80). Six parental inbreds developed from two resistant donors PBC80 and PBC932 were crossed in a haft-diallel fashion to develop 15 cross combinations during 2018. In 2019, seeds of 21 genotypes comprising six parents and 15 F<inf>1</inf> s were planted in Randomized Complete Block Design (RCBD) and data on plant characteristics, fruit yield and yield components were recorded. Detached fruits were screened against anthracnose causing aggressive species (C. acutatum). ANT4 showed the lowest disease lesion size and lowest GCA value at green and red fruit stages, containing resistance from PBC80. Additionally, the general combining ability (GCA) and specific combining ability (SCA) were significantly different at the green fruit stage, with the SCA component greater than GCA. Based on GCA and SCA estimates, ANT4 was selected and crossed to three highly susceptible cultivars (Yodsonkeam 80, Num, and Jindanil 80). The crosses were advanced and 10 F<inf>5</inf> lines were developed through marker-assisted selection (MAS) for resistance genes. The MAS was more useful for selecting resistance at the green fruit stage. We also found that MAS was more effective in F<inf>2</inf> progenies compared to F<inf>5</inf> progenies. Finally, we selected 10 new pure lines and evaluated them at the farmer's field. AF1, AF6, and AF7 were highly resistant to anthracnose, a more desirable good fruit type and high yield. - Some of the metrics are blocked by yourconsent settings
Item type:Publication, Improving Pepper Inbreds for Resistance to Pepper Yellow Leaf Curl Thailand Virus (PepYLCTHV) through Challenged Inoculations(2024-09-01) ;Kumsee, Nattika; ; ;Tsai, Wen ShiTechawongstien, SuchilaChili peppers (Capsicum annuum L.) are an economically important crop worldwide. Pepper yellow leaf curl Thailand virus (PepYLCTHV), a Begomovirus causing yellow leaf mosaic disease of chili pepper, has been reported to incur 95% economic loss under epiphytotic conditions. Thirty-one chili genotypes were screened for resistance to PepYLCTHV disease through inoculation using 10–15 viruliferous whiteflies per plant. We purified two resistant lines (PEP6 and PEP12) through four generations of selfing and selection. At 28 days after inoculations, two chili genotypes (PEP6 and PEP12) had low disease severity and percentage of disease incidence (DI) compared to four susceptible checks, viz., Yodsonkeam80, Homsupan, Huareau12, and Pong Charian, which had a disease severity score of 5 with 100% DI. Thirty initial plants of PEP6 showed an average disease severity of 3.64 with 69.33% DI, and PEP12 showed an average disease severity of 3.83 with 77.67% DI. From these populations, we selected nine highly resistant plant of PEP6 and seven plants of PEP12 having a disease severity of 0 through pure-line selection for four selfing generations. The ratio of resistance (R) to susceptibility (S) consequently decreased. In PEP6, the ratio decreased from 1R:2S to 1R:1S, while in PEP12 the ratio decreased from 1R:3S to 1R:1S. These lines have potential for release as resistant lines for improving chili pepper resistance to PepYLCTHV and for developing makers associated with the resistant trait. - Some of the metrics are blocked by yourconsent settings
Item type:Publication, Preharvest Chitosan Effects on Growth, Yield and Quality of ‘Super Hot’ and ‘Num Khao’ Chili Pepper (Capsicum annuum L.)(2022-01-01); Two chili pepper (Capsicum annuum L.) cultivars ‘Super Hot’ and ‘Num Khao’ grown under field conditions in Thailand were sprayed with 0 (distilled water, control), 25, 50, 100, and 200 ppm chitosan after one week from transplanting and weekly thereafter for five weeks. Chitosan spray improved vegetative growth of both cultivars as measured by increased canopy size. It also increased the plant height of the ‘Super Hot’ cultivar and reduced leaf curl incidence in the ‘Num Khao’ cultivar. Leaf size and chlorophyll content were not significantly affected. Fruit were harvested from cultivars at the commercial maturity stage; that is, the red-ripe stage for ‘Super Hot’ and light green stage for ‘Num Khao’. From the four harvests at weekly intervals, chitosan treatment increased the ‘Super Hot’ yield because of greater fruit production and increased fruit size and there was a higher number of fruit per plant produced by the ‘Num Khao’ cultivar. Fruit color (L*, a*, b*, and sensory color) was not affected, but overall acceptability increased in response to chitosan spray due to improved size and quality of the ‘Super Hot’ cultivar and improved appearance, size and glossiness of the ‘Num Khao’ cultivar. Chitosan at 50 ppm appeared to be the optimum concentration to induce the above effects in both cultivars. - Some of the metrics are blocked by yourconsent settings
Item type:Publication, Effect of Trichoderma harzianum against Fusarium oxysporum in resistant and susceptible tomato cultivars(2025-01-01); ;Mueangkhong, Manthana ;Kawicha, Praphat ;Thanyasiriwat, ThanwanitTechawongstien, SuchilaFusarium wilt disease presents a substantial challenge to tomato production, especially in an open field environment. The peroxidase (POD) activity and total phenolic compounds (TPCs) play a crucial role in measuring the antioxidant capacity of plants. Understanding the variations in the POD and TPC levels during disease-induced stress becomes important for effectively managing Fusarium wilt and enhancing tomato production. This study investigates the impacts of Trichoderma harzianum inoculation through the root drip method on five tomato cultivars. It compares these cultivars to their non-treated counterparts when they are subjected to infection by Fusarium oxysporum f. sp. lycopersici (Fol). The results showed that the level of resistance to Fol is based on the specific tomato cultivar. Notably, 'MT26' exhibited the lowest disease severity index (DSI), indicating a strong response, whereas 'CLN3682F' showed notable susceptibility. Regarding the POD and TPC activity, its exhibition differed in compatibility with the response of each tomato cultivar to Fusarium wilt disease. The resistant cultivars increased the POD activity after the Trichoderma induction before the Fol inoculation, and this activity was further boosted when exposed to disease conditions. Consequently, enhancing the POD and TPC levels during the initial stages could potentially serve as a systemic defence mechanism of tomatoes against the Fusarium wilt disease. - Some of the metrics are blocked by yourconsent settings
Item type:Publication, Phenols and peroxidase activity in Pepper yellow leaf curl Thailand virus (PepYLCThV) resistant and susceptible chili (Capsicum annuum L.) genotypes(2020-07-01) ;Kingkampang, H.; ; ;Techawongstien, S.Chilli (Capsicum annuum L.) is most commonly cultivated species. Pepper yellow leaf curl virus (PepYLCV) causes serious leaf curl disease of chili. In order to understand plant defence mechanism to PepYLCV disease, the study reported a response of total phenols and peroxidase activity in two contrasting pepper genotypes (resistant and susceptible) against PepYLCThV. The total phenolic content of uninoculated plants in susceptible genotype (KKUP31118) showed higher value than resistance genotype (9853-123) at both stages. However, the amount of total phenolic content in resistant genotypes was increased in juvenile and mature leaves in response to inoculation with PepYLCThV. In case of susceptible one, it was decreased as compared to uninoculated control. On the other hand, the peroxidase activity in chilli genotypes and at different stage of leaves was increased every week after inoculation and increased as a comparison to uninoculated plants. Hence, our finding suggested that the total phenols may act as defence mechanism in resistant genotype 9853-123. - Some of the metrics are blocked by yourconsent settings
Item type:Publication, Antioxidant Potential, Phenolic Content, and Nitrate/Nitrite Content in Various Lettuce Varieties(2024-01-01) ;Pumnuan, Jarongsak; ;Sarapothong, Kritima ;Doungnapa, ThanapornPisutpiboonwong, NattakarnTen lettuce varieties are analyzed for antioxidant activity, total phenolic, nitrate, and nitrite contents, and the effects of harvest maturity and cold storage in selected varieties are determined. Antioxidant activity and total phenolic content (TPC) are the highest in Red Oak, Red Coral, Red Rapids, and Grand Rapids; intermediate in Green Oak, Green Cos, and Frillice Iceberg; and the lowest in Butterhead, Mini Green Cos, and Head lettuce. Nitrate content is the highest in Red Coral, while the other 9 varieties have comparably lower contents. Nitrite content is inadequate and does not differ with variety. Harvest maturity of 45 days after transplanting (DAT) produced the highest antioxidant activity and TPC, much higher in Red Bowl and Red Butterhead varieties than in Mini Green Cos, Butterhead, Frillice Iceberg, and Green Big Bowl varieties. Storage at 8<sup>o</sup>C for 21 days has no remarkable effects on antioxidant activity, TPC, nitrate, and nitrite contents. Stored Red Bowl lettuce has higher antioxidant activity and TPC than Butter-head and Green Big Bowl varieties. Nitrate content decreases at the end of storage, while nitrite content is below 1 mg/kg FW during the entire storage period, regardless of variety. - Some of the metrics are blocked by yourconsent settings
Item type:Publication, Differential Biochemical Responses of Resistant and Susceptible Genotypes of Chili to Pepper Yellow Leaf Curl Thailand Virus(2025-09-01) ;Mueangkhong, Manthana; ;Techawongstien, Suchila; Tsai, Wen ShiChili (Capsicum annuum L.) production is threatened by the pepper yellow leaf curl virus (PepLCV), transmitted by whiteflies, leading to reduced yields. This study investigated the biochemical changes in two chili genotypes, PEP6 (tolerant to PepLCV) and Homsuphan (susceptible to PepLCV), following inoculation with the Thailand strain of PepLCV (known as Pepper Yellow Leaf Curl Thailand Virus, PepYLCTHV). Inoculation was performed using whitefly transmission (WF) and graft transmission (GT) methods, and disease severity was evaluated using a standardized index. The level of total phenolic compounds and the activities of peroxidase (POD) and polyphenol oxidase (PPO) enzymes were analyzed in virus-infected plants and compared with those in uninoculated controls. Both chili genotypes exhibited a more rapid increase in disease severity when inoculated with WF than with GT. In PEP6, disease severity was lower than Homsuphan in both WF and GT inoculations. Disease severity in WT-inoculated PEP6 plants increased gradually, reaching 100% by day 36, whereas Homsuphan plants had a more rapid progression, attaining 100% by day 21. The GT method led to slower disease severity progression in both genotypes, reaching 80–85% by day 36. In PEP6 plants, total phenolic compound content increased significantly following WF, indicating an active defense response, whereas levels remained stable in GT plants. Phenolic content in the tolerant genotype Homsuphan remained stable across all conditions. Notably, peroxidase (POD) activity was elevated in GT plants of both genotypes, which correlated with reduced disease severity. Polyphenol oxidase (PPO) activity was lowest in control plants, but WT and GT increased the PPO level. Among the treatments, GT induced the highest PPO activity, which was associated with the lowest disease severity. These findings suggest that GT may enhance disease resistance by modulating phenolic compound accumulation and increasing POD and PPO activity. - Some of the metrics are blocked by yourconsent settings
Item type:Publication, Growth and Yield of Watermelon (Citrullus lanatus) in Plastic House in Response to White LED Supplementary Lighting(2023-01-01) ;Chamchum, Wasinee ;Glahan, Somchai; ;Maniwara, PhonkritWatermelon plants cultivar ‘Kinaree 457’ were grown in plastic house under natural daylight only (control) or with nightly LED supplementary lighting for 6 h (6:00 pm-12:00 pm) or 12 h (6:00 pm-6:00 am) starting from transplanting up to fruit harvest. Plant height, leaf chlorophyll content and fruit yield significantly increased in response to 6 h supplementary LED lighting. Fruit mass, size (length x width) and flesh thickness at 6 h LED treatment were about 2.3 kg, 19.3 ×15.7 cm, and 15.7 cm, respectively, while the fruit of control had 1.7 kg, 16.0 × 14.3 cm, and 13.8 cm, respectively. No significant treatment effect was obtained on peel thickness, flesh color L* and b* values, juice pH and total soluble solids. However, 6 h LED treatment resulted in lower reddening flesh (lower a* values), firmness and higher titratable acidity relative to the control, suggesting the need for improvement in cultural management. Furthermore, multivariate statistics of principal component analysis (PCA) performed on physico-chemical quality revealed the variations among watermelons from lighting and control treatments regardless of lighting hour.
