Poeaim, Supattra

Preferred name

Poeaim, Supattra

Alternative Name

Poeaim, S.

Poeaim, Supattar

Main Affiliation

School of Science

supattra.poe@kmitl.ac.th

Now showing 1 - 10 of 57

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Chaetomium siamense sp. nov., a soil isolate from Thailand, produces a new chaetoviridin, G
(2011-01-01)
Pornsuriya, Chaninun
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Soytong, Kasem
;
Poeaim, Supattar
;
Kanokmedhakul, Somdej
;
Khumkomkhet, Primmala
Chaetomium siamense sp. nov., isolated from soil in a pineapple plantation in Thailand, is described and illustrated. The new species is similar to C. cupreum in having superficial globose or ovate ascomata with red circinate ascomatal hairs but can be distinguished by its fusiform ascospores with two apical germ pores. Phylogenetic analysis of ITS rDNA sequence data also supports C. siamense as distinct. Investigation of the secondary metabolites of C. siamense led to the isolation of one new (chaetoviridin G) and seven known compounds (ergosterol, 24(R)-5á,8á-epidioxyergosta-6-22-diene- 3â-ol, ergosterylplamitate, cochliodone D, chaetoviridin A, chaetoviridin F, chrysophanol). © 2011. Mycotaxon, Ltd.
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Antifungal activity of microbial nanoparticles derived from Chaetomium spp. against Magnaporthe oryzae causing rice blast
(2020-01-01)
Song, Jiao Jiao
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Soytong, Kasem
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Kanokmedhakul, Somdej
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Kanokmedhakul, Kwanjai
;
Poeaim, Supattra
The Magnaporthe oryzae isolate PO2 was proven to cause rice blast var. PSL 2 in this study. Chaetomium elatum, Chaetomium lucknowense and Chaetomium brasiliense were antagonised to M. oryzae. The hexane extract of C. brasiliense gave the highest inhibition of the spore production with an ED<inf>50</inf> of 35 ppm, the EtOAC extract of C. lucknowense inhibited the spore production at 57 ppm and the EtOAC extracts of C. elatum inhibited the spore production at 106 ppm. The nano-CLM (C. lucknowense) inhibited the spore production at 5.24 ppm, the nano-CBH (C. brasiliense) inhibited the spore production at 6.86 ppm and the nano-CEE (C. elatum) inhibited the spore production at 7.89 ppm. The rice leaves treated with nano-CBH from C. brasiliense produced Sakuranertin and Oryzalexin B as seen on the thin layer chromatography where the Rf value was 0.08 assumed to be Sakuranertin, and the R<inf>f</inf> value of 0.28 supposed to be Oryzalexin B. It was found that the nanoparticles act as elicitors to induce immunity in rice plants through the production of phytoalexin.
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Effective of Neosartorya and Talaromyces to control Alternaria brassicicola causing leaf spot of kale
(2018-12-01)
Punyanobpharat, A.
;
Soytong, K.
;
Poeaim, S.
The antagonistic potential of Neosartorya and Talaromyces proved to control leaf spot of kale caused by Alternaria brassicicola. Leaf spot symptoms were collected from Nakornpathom, Suphanburi, Ratchaburi and Nonthaburi provinces, isolated and identified the pthogen by morphological and molecular characterization. Four isolates were confirmed as A. brassicicola. The pathogenicity test was done by detached leaf bioassay and found that AbK-NP01 was the highest virulence pathogenic isolate. Dual culture technique was used to assay with eight species of antagonistic fungi (N. hiratsukae, N. pseudofischeri, N. aureola, N. spinosa, N. fennelliae, Neosartorya sp., T. trachyspermus and T. muroii) against A. brassicicola. The best effective of antagonist to control the tested pathogen was N. spinosa CHA09-A01.
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Investigation of Biodegradation and Biocompatibility of Chitosan–Bacterial Cellulose Composite Scaffold for Bone Tissue Engineering Applications
(2025-05-01)
Yodsanga, Somchai
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Poeaim, Supattra
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Chantarangsu, Soranun
;
Swasdison, Somporn
Developing scaffolds with a three-dimensional porous structure and adequate mechanical properties remains a key challenge in tissue engineering of bone. These scaffolds must be biocompatible and biodegradable to effectively support osteoblastic cell attachment, metabolic activity, and differentiation. This study successfully fabricated a chitosan–bacterial cellulose (CS–BC) composite scaffold using the solvent casting/particle leaching (SCPL) technique, with NaOH/urea solution and sodium chloride crystals as the porogen. The scaffold exhibited a well-distributed porous network with pore sizes ranging from 300 to 500 µm. Biodegradation tests in PBS containing lysozyme revealed a continuous degradation process, while in vitro studies with MC3T3-E1 cells (pre-osteoblastic mouse cell line) demonstrated excellent cell attachment, as observed through SEM imaging. The scaffold also promoted increased metabolic activity (OD values) in the MTT assay, and enhanced alkaline phosphatase (ALP) activity and upregulated expression of osteogenic-related genes. These findings suggest that the CS–BC composite scaffold, fabricated using the SCPL method, holds great potential as a candidate for bone tissue engineering applications.
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A new nanofibre derived from Trichoderma hamatum K01 to control durian rot caused by Phytophthora palmivora
(2023-01-01)
Phal, Pheaktra
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Soytong, Kasem
;
Poeaim, Supattra
Phytophthora rot of durian var Monthong caused by Phytophthora palmivora has been proven to be a serious threat to durian plantations in Thailand. The research was targeted to isolate the causal pathogen and prove its pathogenicity by Koch’s postulate. Morphology and molecular phylogeny have confirmed the identification of pathogenic and antagonistic fungi. Evaluation of antagonistic fungus against plant pathogen in vitro and greenhouse conditions, morphology and molecular phylogenetic identification confirmed antagonistic species Trichoderma hamatum K01 and pathogenic isolate P. palmivora PYSC01. The crude metabolite of T. hamatum K01, namely TK01-MeOH gave the most substantial inhibitory effect to inhibit colony growth and sporangia formation at ED<inf>50</inf> (50% effective dose) values of 288 and 118 µg/mL, respectively. Moreover, nanofibre namely nano-TK01M exhibited the best antifungal activity in inhibiting colony growth and sporangia formation at ED<inf>50</inf> values of 11 and 3 µg/mL, respectively. Nano-TK01M treated on durian leaves induced the synthesis of scopoletin, which is known as a defense mechanism and marker of plant resistance or plant immunity. Moreover, the application of nano-TK01M significantly reduced disease incidence, the same as metalaxyl. Additionally, nano-TK01M treatment was the most effective in enhancing plant physiological parameters, including the synthesis of chlorophyll, carotenoid contents and promoted plant growth, compared to both metalaxyl and non-treated control. T. hamatum K01 produced antifungal metabolite pyrone 6-pentyl-2H-Pyran-2-one and sorbicillin. It is reported for the first time that pyrone and sorbicillin could be expressed as bioactive compounds in reduction of the disease incidence of durian rot caused by P. palmivora. The finding confirmed that nano-TK01M from T. hamatum K01 exhibited the most effective in controlling plant pathogen, which could be promoted as agricultural input for plant disease management, and it is also a nontoxic fungicide for living life and eco-friendly.
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Antibacterial and anti-tyrosinase activities of the methanolic extracts from leaves of Tectona grandis
(2018-12-01)
Phonmakham, J.
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Wattanasuksakul, S.
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Poeaim, S.
The antibacterial and anti-tyrosinase activities of methanolic extracts from fresh and fallen leaves of Tectona grandis (Teak) was studied. Fresh and fallen of teak leaves were collected from Thongphaphum and Phitsanulok silviculture research station. Antibacterial activity was determined by disc diffusion method with concentration at 500 µg/disc. Fresh and fallen of teak leaves extracts showed a good activity against Staphylococcus aureus and Staphylococcus epidermidis. In the other hand, fresh teak leaves inhibited growth of Propionibacterium acnes better than fallen teak leaves. The anti-tyrosinase activity was determined by Dopachrome method with concentration at 1500 µg/ml, inhibitory power of tyrosinase enzyme from fresh and fallen leaves extracts value were detected in the percentage between 35.45%-73.65%.
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Biological activities of the methanolic extracts from two varieties of Dimocarpus longan seeds
(2018-12-01)
Natungnuy, K.
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Chareonsap, P. P.
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Poeaim, S.
Dimocarpus longan belongs to Sapindaceae which two varieties are found in Thailand. In addition to commercial longan (D. longan ssp. longan var. longan), in the eastern part of Thailand found another type of flora longan called Thao (D. longan ssp. longan var. obtusus). The total phenolic content and various biological activities was investigated including antioxidant, antibacterial, anti-tyrosinase and cytotoxic activities of the methanolic extracts from two varieties of these longan seeds. The results showed that the most activity of Edor (var. longan) seed extract presented higher biological activities than Thao (var. obtusus) seed extract. The total phenolic content by Folin-Ciocalteu method related to antioxidant activity in DPPH, ABTS and FRAP assays. In antibacterial activity using disc diffusion method both varieties of extracts (5 mg/disc) inhibited Staphylococcus aureus, Micrococcus luteus and Bacillus subtilis. However, in anti-tyrosinase activity by the dopachrome method Thao revealed similar activity to the commercial longan cultivar Edor with 50% inhibitory concentration (IC <inf>50</inf> ) values of 504.73 and 527.04 µg/ml, respectively. In cytotoxic activity using MTT assay with L929 (Mouse fibroblast cell line), Thao seed extracts were non-cytotoxic activity and stimulated skin cell lines, while Edor showed low cytotoxic activity. Therefore, their bioactive compounds should be studied further for developing longan seed extracts to cosmetic and pharmaceutical products in the future.
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Four new species of genus Acmella W.T. Blanford, 1869 (Gastropoda, Assimineidae) from Southern Thailand
(2025-01-01)
Seedee, Kunya
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Dumrongrojwattana, Pongrat
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Poeaim, Supattra
This study explores the diversity of microsnails inhabiting limestone caves in Southern Thailand. It describes four new species of the genus Acmella W.T. Blanford, 1869 (Gastropoda, Assimineidae): Acmella krueangensis sp. nov. from Ranong Province, A. thamsingensis sp. nov. and A. changphueakensis sp. nov. from Chumphon Province, and A. kanchanaditensis sp. nov. from Surat Thani Province. These species are distinguished by shell morphology, particularly the number of striae on the last whorl and the protoconch sculpture. Phylogenetic analyses based on mitochondrial cytochrome c oxidase subunit I (COI) gene sequences further support their distinctiveness and clarify their taxonomic placement. The results enhance the understanding of Acmella diversity in Thailand and shed light on biogeographical patterns found in this genus and the adaptations needed to survive under the conditions of cave systems.
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Plant regeneration of Bauhinia purpurea by tissue culture technique
(2024-05-01)
Saparam, W.
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Poeaim, A.
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Pongtongkam, P.
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Chareonsap, P. P.
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Poeaim, S.
Results showed that the seed of Bauhinia purpurea germination was archived 100% from half-strength MS medium supplemented with 3.0 mg/L Gibberellic acids (GA3) and gave 71.137 mm for the highest seedling. The optimal condition of nodal explants sterilization was E5, which gave 92% of survival explants. For the shoot induction method, the results showed that 24.270 mm was the highest proliferation of shoots achieved from MS medium supplemented with 1.0 mg/L 6-benzylaminopurine (BAP). The highest number of roots was 2.867 roots, and the highest root length was 33.453 mm, obtained at half-strength MS medium complemented with 0.25 mg/L Indole-3-acetic acid (IAA). The 73.33% of plantlets were survived after being transferred to plastic pot.
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Sequence-related amplified polymorphism (SRAP) analysis for studying genetic characterization of bouea macrophylla
(2016-10-01)
Kaewpongumpai, Sombhat
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Poeaim, Supattra
;
Vanijajiva, Ongkarn
Kaewpongumpai S, Poeaim S, Vanijajiva O. 2016. Sequence-Related Amplified Polymorphism (SRAP) analysis for studying genetic characterization of Bouea macrophylla. Biodiversitas 17: 539-543. Bouea macrophylla Griff. is well-known as one of native typical fruits in Southeast Asia which needs to be preserved and continuously cultivated because of economical and ecological significances. More recently, sequence-related amplified polymorphism (SRAP) markers have been developed, which are used to amplify coding regions of DNA with primers targeting open reading frames. This technique has proven to be robust and highly variable and is attained through a significantly less technically demanding process. In this research, SRAP method was preliminary applied to assess genetic characterization of B. macrophylla. Genomic DNA was extracted from fresh leaf samples. The result clearly showed that at 100 ng template DNA and MgCl<inf>2</inf> 5 mM concentration are suitable for further PCR analysis. Thirty SRAP primer combinations were initially screened for analysis and 26 primer combinations were chosen for further analysis. A total of 222 DNA fragments, varying from 90-2500 bp, were amplified. The produced band number for each optimal primer set ranged from 3 to 12 with a percentage of polymorphic bands spanning from 33.33 to 80.00%. Therefore, SRAP analysis is suitable for further analysis method on genetic study of Bouea species and related genera.

Poeaim, Supattra

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