Srikijkasemwat, Kanokrat

Preferred name

Alternative Name

Srikijkasemwat, K.

Main Affiliation

School of Agricultural Technology

kanokrat.sr@kmitl.ac.th

Now showing 1 - 4 of 4

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Evaluation of Productivity and Egg Quality in Japanese Quails Reared Under Different LED Colors and Rearing Systems
(2026-02-01)
Kaewhom, Paitoon
;
Saelim, Kraiyot
;
Poolsamran, Patcharawadee
;
Thammakarn, Chanathip
;
Chaosap, Chanporn
This study evaluated the productivity and egg quality of Japanese quails reared under different LED colors and rearing systems. A total of 720 female quails were assigned to a 3 × 2 factorial arrangement with three LED colors (red, green, and white) and two rearing systems (cage and floor) until 20 weeks of age. Production performance was evaluated across specific age intervals, while physical egg quality traits were analyzed using a Repeated-measures General Linear Model to assess temporal changes. No significant overall interactions between LED color and rearing system were observed (p > 0.05). However, significant interactions between treatment and time (p < 0.05) revealed that red LED light progressively enhanced productivity, while the floor system significantly improved feed efficiency and income during the early laying phase (weeks 6–12). Specifically, red LED light significantly improved hen-day production, egg mass, feed efficiency, and income-to-cost ratio compared to other colors (p < 0.05). Physical egg quality traits remained consistent across treatments (p > 0.05) but were significantly influenced by time (p < 0.05). In conclusion, red LED light optimizes long-term profitability, whereas the floor system offers distinct advantages during the onset of lay.
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Seroprevalence of Porcine Circovirus Type 2 in Domestic Pigs of Thailand: Two Decades After Nationwide Vaccines Application
(2022-12-01)
Thammakarn, Chanathip
;
Umphonphison, Sawanya
;
Kaewhom, Paitoon
;
Srikijkasemwat, Kanokrat
The antibody response of domestic pigs to porcine circovirus type 2 (PCV2) was investigated in commercial farms at various regions of Thailand during the year 2018-2021. A total 2,002 serum samples were collected from 16 farms located in 10 provinces and tested by Enzyme-Linked Immunosorbent Assay. Among total serum samples, 914 samples were further tested by polymerase chain reaction (PCR) to detect ORF2 gene. The results revealed that 81.17% of pigs had low positive level antibody against PCV2, while 10.29% had high positive titer. On the other hand 8.54% of pig had no antibody to PCV2. The low positive antibody level was found in most population in all farm sizes as well as all types of pig as in age categories. The PCR result illustrated that there was no viral gene in seronegative serum. While 0.8% of total low positive serum samples displayed positive by PCR. In addition, 3.23% of total high positive serum samples showed positive by PCR. The present study is the first nationwide serological prevalence of PCV2 antibody report since two decades of PCV2 vaccines was applied in Thailand. The results proved that high prevalence of PCV2 immunity was established, with the most population had antibody titer fall in low positive, while some of viremic pigs still presented, determined that the routine vaccination programs used in farms can control PCV2 efficiently since continuing vaccination.
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Enhancing the shelf-life of washed eggs using a gelatin-based coating enriched with pomegranate peel extract under tropical storage conditions
(2026-06-01)
Thiangtae, Siwimol
;
Srikijkasemwat, Kanokrat
;
Kaewhom, Paitoon
;
Kongrith, Chunya
;
Polyorach, Sineenart
Egg washing effectively improves food safety but accelerates quality deterioration by removing the natural cuticle, especially during ambient storage. This study investigated the efficacy of a gelatin-based edible coating incorporated with pomegranate peel extract (PPE) in preserving the internal quality of washed chicken eggs stored at room temperature (25–30°C). Eggs were assigned to four treatments: uncoated control (UC), gelatin-only (GEL), and gelatin containing 1.0% (GEL-PPE1.0) or 1.5% PPE (GEL-PPE1.5), and evaluated over 28 days. The results showed that GEL-PPE1.5 significantly outperformed other groups in preserving internal quality (P ' 0.05). By Day 28, the GEL-PPE1.5 group maintained a Haugh unit (HU) of 55.59 ± 9.42, whereas the UC group dropped to 33.73 ± 17.29 (P ' 0.001). Similarly, the yolk index (YI) of the GEL-PPE1.5 group was 0.21 ± 0.02, compared to 0.11 ± 0.03 in UC eggs. The PPE coatings also moderate pH shifts; the albumen pH of the GEL-PPE1.5 group remained at 9.16 ± 0.20, significantly lower than the 9.61 ± 0.14 in the UC group (P ' 0.05). No microbial growth was detected in any treatment (' 10 CFU/g). The preservation efficacy arises from synergistic mechanisms: the gelatin matrix acts as a physical barrier to gas exchange, while the ellagic acid-rich PPE enhances biochemical stability by mitigating oxidative degradation. These findings demonstrate that PPE-fortified gelatin coatings provide a practical and bioactive strategy for maintaining egg freshness, offering a clear magnitude of protection for washed eggs in supply chains where refrigeration is limited.
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Efficacy of two primer sets used in the sex identification of rufous-winged buzzard (Butastur liventer)
(2021-01-01)
Kaewhom, Paitoon
;
Srikijkasemwat, Kanokrat
Background: The rufous-winged buzzard (Butastur liventer) belongs to the order Accipitriformes, which is monomorphic, resulting in the difficulty to identify the gender. However, sex determination is important for predicting the sex ratio of this buzzard in nature in order to avoid its extinction. Aim: We aimed to develop a primer set that is able to sex the rufous-winged buzzard through polymerase chain reaction (PCR) amplification and compare the efficacy of the two sets of primers by using PCR technique. Methods: In the following, sensitivity refers to the smallest DNA concentration that allowed us to accurately sex a bird and specificity refers to the ability to clearly distinguish the sex based on the visual appearance of the bands. Blood samples were collected from captive buzzards. The DNA was extracted from them and was diluted to 50, 25, 10, 5, 2.5, 1.67, and 1 ng/µl. Two sets of primers, including P2/NP/MP and 2550F/2718R, were used to amplify the chromo-helicase DNA binding (CHD) gene of known gender buzzards using the PCR process to determine gender and to compare their sensitivity. To measure specificity, both primers were used to amplify CHD gene fragment of other unknown gender birds. Results: The lowest concentration of the DNA template where P2/NP/MP could amplify DNA fragments was 1 ng/µl, and this set of primers could identify the gender of all birds correctly, giving 100% specificity. On the other hand, the 2550F/2718R could amplify the DNA fragments from 5 ng/µl, and it had only 78% specificity. Conclusion: The P2/NP/MP primer set was able to correctly identify the gender of rufous-winged buzzard through PCR amplification with high specificity and sensitivity.

Srikijkasemwat, Kanokrat

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